Molecular identification of a Pm4 allele conferring powdery mildew resistance in durum wheat DR88 DOI Creative Commons
Guohao Han,

Lixian Xing,

Tiantian Gu

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 6, 2024

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most destructive wheat diseases worldwide. Durum (Triticum turgidum L. var. durum Desf.) a crucial gene donor for improving common wheat. In this study, we investigated accession, DR88, which exhibits broad and high levels resistance to powdery mildew. Using bulked segregant RNA-Seq (BSR-Seq), identified dominant gene, tentatively designated PmDR88, localized it 743–776 Mb interval on chromosome arm 2AL according reference genome cv. Svevo. Subsequently, PmDR88 was mapped in genetic region 3.9 cM flanked markers WGRE77410 WGRC872 at distances 1.6 2.3 cM, respectively; also co-segregated with JS717×JS718, diagnostic marker Pm4 locus. Genotyping large population comprising 5,174 F2:3 families using JS717×JS718 confirmed that located locus 2AL. Sequence alignment revealed shares identical amino acid sequences Pm4d, while qRT-PCR analysis suggested distinct expression patterns compared previously reported alleles. Two complementary DNA markers, including co-segregating newly developed closely-linked co-dominant WGRE77410, were be available efficiently transferring into tested backgrounds marker-assisted selection (MAS) strategy. Despite sharing patterns. Moreover, DR88 shows MAS breeding. The molecular identification will facilitate transfer allele susceptible cultivars improvement or resistant resistance-enhanced pyramiding

Language: Английский

Fine-mapping of PmHHM, a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew DOI Creative Commons

Bisheng Fu,

Zhen Lin, Lijuan Yan

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 6, 2025

Common wheat is a leading global food crop that impacts security. Wheat powdery mildew (PM), caused by Blumeria graminis f. sp. tritici (Bgt), poses significant threat to grain yield and flour quality. The identification utilization of broad-spectrum resistance genes against PM are essential for effective disease control. spectrum test during the seedling stage adult were conducted evaluate landrace Honghuamai (HHM). Five segregating populations investigated assess inheritance in HHM. To map its resitance gene, bulked segregant analysis, molecular mapping comparative genomic analysis also used present study. HHM shows remarkable field nearly immune all 25 Bgt isolates tests, making it an excellent source resistance. was determined single dominant temporarily named PmHHM. It then fine-mapped interval with genetic distance 0.0031 cM physical 187.4 kb on chromosome 4AL Chinese Spring reference sequence v.2.1. Four identified target region, three which encode nucleotide-binding leucine-rich repeat (NLR) proteins. Comparative revealed presence/absence variations (PAVs) PmHHM locus among common varieties. These closely linked markers will not only benefit cloning gene underlying but facilitate efficient breeding programs.

Language: Английский

Citations

0
Development and characterization of small translocations between the Pm13 introgression from Aegilops longissima and wheat homoeologous A, B and D chromosomes DOI Creative Commons
Shunzong Ning,

Xiyou Wang,

Chunfang Ma

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 11, 2025

Wild relatives have substantial impacts on the resistance of wheat to biotic and abiotic stresses. The genetic diversity these wild varieties can be employed widen gene pool by introducing allele introgression genome structure variations. powdery mildew Pm13 was derived from relative species Aegilops longissima (S1S1, 2n = 2x 14) transferred into chromosome 3BS over 30 years. Until recently, it has not been widely used in breeding, possibly because linkage drag associated with or poor alien segment compensation 3BS. In this study, translocations between homoeologous A, B D chromosomes were developed using a ph1b mutant. 3Sl (3B) substitution line hybridized CS nullisomic-tetrasomic N3AT3B, then F1 CSph1b Individuals homozygous for mutant carrying identified segregating populations via molecular markers cytological analysis. Whole-arm recombinant T3SlS.3AL T3SlS.3BL small T3SlS-3AS.3AL, T3SlS-3BS.3BL T3DS-3SlS-3DS.3DL subsequently Furthermore, 4 disomic recombinants identified. All nearly immune mildew. Our results provide novel improving insights interactions different genomes segments.

Language: Английский

Citations

0
Advancing wheat breeding using rye: a key contribution to wheat breeding history DOI Creative Commons

G C Han,

Hanwen Yan,

LI Li-hui

et al.

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Analysis of structural and metabolic changes in surface microorganisms following powdery mildew infection in wheat and assessment of their potential function in biological control DOI Creative Commons

Zhen Wu,

Xiaodong Xue

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(4), P. e0320682 - e0320682

Published: April 22, 2025

Powdery mildew is a highly destructive disease that greatly reduces both the yield and quality of wheat. As there limited research on changes in microorganism community caused by powdery infection different tissue parts, especially after spike infection, this study aimed to examine surface microorganisms infected healthy wheat plants. Samples were collected from leaves spikes, number operational taxonomic units (OTUs), diversity index, abundance, metabolic microbial analysed using 16S rRNA amplicon sequencing technology. Through identification tissues, 24 phyla identified leaves, 20 spikes. The dominant bacterial observed Proteobacteria Bacteroidetes . At genus level, 19 genera detected 11 Notably, total exceeded Pseudomonas , Sphingomonas Pantoea species 37 types 35 included Pedobacterium panaciterrae baetica rhizophaerae aerolata analysis conducted revealed incidence was greater plots situated closer obstacles than other plots. when with mildew, results indicated spikes significantly impacted, response being more pronounced leaves. Different leaf surface, pathway are mainly regulation. These provide theoretical support for prevention control crops.

Language: Английский

Citations

0
LpY3IP1 Enhances the drought and salt tolerance of perennial ryegrass by protecting the photosynthetic apparatus DOI

Wenfei Xie,

Zhijian Cao, Yilin Zhao

et al.

Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 338, P. 113645 - 113645

Published: Sept. 10, 2024

Language: Английский

Citations

0
Molecular identification of a Pm4 allele conferring powdery mildew resistance in durum wheat DR88 DOI Creative Commons
Guohao Han,

Lixian Xing,

Tiantian Gu

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 6, 2024

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most destructive wheat diseases worldwide. Durum (Triticum turgidum L. var. durum Desf.) a crucial gene donor for improving common wheat. In this study, we investigated accession, DR88, which exhibits broad and high levels resistance to powdery mildew. Using bulked segregant RNA-Seq (BSR-Seq), identified dominant gene, tentatively designated PmDR88, localized it 743–776 Mb interval on chromosome arm 2AL according reference genome cv. Svevo. Subsequently, PmDR88 was mapped in genetic region 3.9 cM flanked markers WGRE77410 WGRC872 at distances 1.6 2.3 cM, respectively; also co-segregated with JS717×JS718, diagnostic marker Pm4 locus. Genotyping large population comprising 5,174 F2:3 families using JS717×JS718 confirmed that located locus 2AL. Sequence alignment revealed shares identical amino acid sequences Pm4d, while qRT-PCR analysis suggested distinct expression patterns compared previously reported alleles. Two complementary DNA markers, including co-segregating newly developed closely-linked co-dominant WGRE77410, were be available efficiently transferring into tested backgrounds marker-assisted selection (MAS) strategy. Despite sharing patterns. Moreover, DR88 shows MAS breeding. The molecular identification will facilitate transfer allele susceptible cultivars improvement or resistant resistance-enhanced pyramiding

Language: Английский

Citations

0