Mutations in Gordonia amarae mycolic acid biosynthetic pathway confer resistance to Patescibacteria parasite Mycosynbacter amalyticus

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 5, 2025

The obligate necrotrophic parasite, Candidatus Mycosynbacter amalyticus, a member of the Patescibacteria has been isolated from wastewater. Subsequent efforts have directed toward unravelling its biological lifecycle and attachment mechanism facilitating infection subsequent lysis Actinobacterial host, Gordonia amarae. Here, using electron cryo-tomography (CryoET), we reveal molecular anatomy parasitic amalyticus cells, uncovering an unusual process. Through laboratory-based evolution experiments, generated eleven slow-growing independent spontaneous amarae resistant mutants. Mycolic acids (MA) are key components outer cellular envelope G. other Actinobacteria, with MA being physical attribute implicated in associated wastewater foaming. CryoET genome sequencing exposed absence intact suite mutations predominantly occurring within pks13 pptT genes biosynthetic pathway. Our findings suggest that structural integrity is critical for Ca. to host.

Language: Английский

Fly-fishing for flagella

Science, Journal Year: 2024, Volume and Issue: 386(6719), P. 273 - 274

Published: Oct. 17, 2024

Molecular grappling hooks are the tools of a sticky bacterial predator.

Language: Английский

Lineage-dependent partitioning of activities in chemoclines defines Woesearchaeota ecotypes in an extreme aquatic ecosystem

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: Nov. 29, 2024

Language: Английский