An Optimized Liquid Chromatography–Mass Spectrometry Method for Ganglioside Analysis in Cell Lines DOI Creative Commons
Akeem Sanni, Andrew I. Bennett, Yifan Huang

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(19), P. 1640 - 1640

Published: Oct. 2, 2024

Gangliosides are glycosphingolipids composed of a sialylated glycan head group and ceramide backbone. These anionic lipids form lipid rafts play crucial roles in regulating various proteins involved signal transduction, adhesion, cell-cell recognition. Neuroblastoma, pediatric cancer the sympathetic nervous system, is treated with intensive chemotherapy, radiation, an antibody targeting GD2 ganglioside. critical neuroblastoma development serve as therapeutic targets, making it essential to establish reliable, rapid, cost-effective method for profiling gangliosides, particularly one capable isomeric separation intact species. In this study, liquid chromatography-mass spectrometry (LC-MS) was optimized using standard followed by optimization sphingolipid extraction methods from cell lines comparing Folch absolute methanol techniques. Percent recovery number identified sphingolipids were used evaluate analytical merits these methods. A gangliosides calibration curve demonstrated excellent linearity (R

Language: Английский

Strategies to Overcome Antigen Heterogeneity in CAR-T Cell Therapy DOI Creative Commons
Bohan Zhang, Jiawen Wu,

Hua Jiang

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(5), P. 320 - 320

Published: Feb. 20, 2025

Chimeric antigen receptor (CAR) gene-modified T-cell therapy has achieved significant success in the treatment of hematological malignancies. However, this not yet made breakthroughs solid tumors and still faces issues resistance relapse cancers. A major reason for these problems is antigenic heterogeneity tumor tissues. This review outlines encountered CAR-T cell corresponding strategies to address it. These include using combination increase abundance target antigens, optimizing structure CARs enhance sensitivity low-density developing multi-targeted cells, reprogramming TME activate endogenous immunity. approaches offer new directions overcoming therapy.

Language: Английский

Citations

1
The current socioeconomic and regulatory landscape of immune effector cell therapies DOI Creative Commons
Chiranjeevi Sainatham, Devvrat Yadav, Aravind Dilli Babu

et al.

Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11

Published: Dec. 4, 2024

Immune cell effector therapies, including chimeric antigen receptor (CAR)-T cells, T-cell (TCR) T natural killer (NK) and macrophage-based represent a transformative approach to cancer treatment, harnessing the immune system target eradicate malignant cells. CAR-T therapy, most established among these, involves engineering cells express CARs specific antigens, showing remarkable efficacy in hematologic malignancies like leukemias, B-cell lymphomas, multiple myeloma. Similarly, TCR-modified which reprogram recognize intracellular tumor antigens presented by major histocompatibility complex (MHC) molecules, offer promise for range of solid tumors. NK-cell therapies leverage NK cells' innate cytotoxicity, providing an allogeneic that avoids some immune-related complications associated with T-cell-based therapies. Macrophage-based still early stages development, focus on reprogramming macrophages stimulate response against microenvironment. Despite their promise, socioeconomic regulatory challenges hinder accessibility scalability These treatments are costly, currently exceeding $400,000 per patient, creating significant disparities access based status geographic location. The high manufacturing costs stem from personalized, labor-intensive processes harvesting, modifying, expanding patients' Moreover, logistics delivering these limit reach, particularly low-resource settings. Regulatory pathways further complicate landscape. In United States., Food Drug Administrations' (FDA) accelerated approval cell-based facilitate innovation but do not address cost-related barriers. Europe, European Medicines Agency (EMA) offers adaptive pathways, yet decentralized reimbursement systems create uneven across member states. Additionally, differing standards quality control worldwide pose hurdles global harmonization access. To expand reach multipronged is needed-streamlined frameworks, policies reduce treatment costs, international collaborations standardize manufacturing. Addressing obstacles essential make life-saving accessible broader patient population worldwide. We present literature review current landscape barriers approved standard care therapy at various levels.

Language: Английский

Citations

4
Exploring neuroblastoma’s cellular microenvironment: A novel approach using cellular automata to model Celyvir treatment DOI Creative Commons
José García Otero, Juan Belmonte-Beitia, Juan Jiménez-Sánchez

et al.

Computers in Biology and Medicine, Journal Year: 2025, Volume and Issue: 188, P. 109782 - 109782

Published: Feb. 12, 2025

Language: Английский

Citations

0
Principles of Immunotherapy and Novel Immunotherapeutic Agents for Pediatric Cancer Management DOI
Zachary Morrison, Ali Suliman

Published: Jan. 1, 2025

Language: Английский

Citations

0
Biomarker-Driven Approaches to Bone Metastases: From Molecular Mechanisms to Clinical Applications DOI Creative Commons

Youssef Elshimy,

Ala’a Aldeen Alkhatib,

Bilal Atassi

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(5), P. 1160 - 1160

Published: May 10, 2025

Bone metastases represent a critical complication in oncology, frequently indicating advanced malignancy and substantially reducing patient quality of life. This review provides comprehensive analysis the complex interactions between tumor cells bone microenvironment, emphasizing relevance "seed soil" hypothesis, RANK/RANKL/OPG signaling axis, Wnt pathways that collectively drive metastatic progression. The molecular cellular mechanisms underlying formation osteolytic osteoblastic lesions are examined detail, with particular focus on their implications for associated breast, prostate, lung, other cancers. A central component this is categorization pathological biomarkers into four types: diagnostic, prognostic, predictive, monitoring. We provide evaluation circulating (CTCs), turnover markers (such as TRACP-5b CTX), imaging (including PET/CT MRI), novel genomic signatures. These offer valuable insights early detection, enhanced risk stratification, optimized therapeutic decision-making. Furthermore, emerging strategies immunotherapy bone-targeted treatments discussed, highlighting potential biomarker-guided precision medicine to enhance personalized care. distinctiveness lies its integrative approach, combining fundamental pathophysiological latest developments biomarker discovery innovation. By synthesizing evidence across various cancer types categories, we cohesive framework aimed at advancing both scientific understanding clinical management metastases.

Language: Английский

Citations

0
An Optimized Liquid Chromatography–Mass Spectrometry Method for Ganglioside Analysis in Cell Lines DOI Creative Commons
Akeem Sanni, Andrew I. Bennett, Yifan Huang

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(19), P. 1640 - 1640

Published: Oct. 2, 2024

Gangliosides are glycosphingolipids composed of a sialylated glycan head group and ceramide backbone. These anionic lipids form lipid rafts play crucial roles in regulating various proteins involved signal transduction, adhesion, cell-cell recognition. Neuroblastoma, pediatric cancer the sympathetic nervous system, is treated with intensive chemotherapy, radiation, an antibody targeting GD2 ganglioside. critical neuroblastoma development serve as therapeutic targets, making it essential to establish reliable, rapid, cost-effective method for profiling gangliosides, particularly one capable isomeric separation intact species. In this study, liquid chromatography-mass spectrometry (LC-MS) was optimized using standard followed by optimization sphingolipid extraction methods from cell lines comparing Folch absolute methanol techniques. Percent recovery number identified sphingolipids were used evaluate analytical merits these methods. A gangliosides calibration curve demonstrated excellent linearity (R

Language: Английский

Citations

0