Destined for destruction: The role of methionine aminopeptidases and plant cysteine oxidases in N-degron formation DOI Creative Commons
Andrea Fuentes-Terrón, Rebecca Latter, Samuel Madden

et al.

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 197(1)

Published: Dec. 23, 2024

Abstract The cysteine/arginine (Cys/Arg) branch of the N-degron pathway controls stability certain proteins with methionine (Met)-Cys N-termini, initiated by Met cleavage and Cys oxidation. In seeding plants, target include Group VII Ethylene Response Factors, which initiate adaptive responses to low oxygen (hypoxic) stress, as well Vernalization 2 (VRN2) Little Zipper (ZPR2), are involved in endogenous developmental hypoxia. It is essential that these only degraded under appropriate physiological conditions. Modification their N-termini enzymatic control Aminopeptidases (MetAPs) Plant Cysteine Oxidases (PCOs); therefore, substrate-binding requirements catalytic effectiveness enzymes important for defining Met-Cys–initiating degraded. Physiological conditions can also impact activity enzymes, well-characterized sensitivity PCOs ensures stabilized this review we compile functional structural properties MetAPs PCOs, including interactions substrates. We consider evolution through plant kingdom highlight role controlling initial steps pathway.

Language: Английский

Illuminating the impact of N-terminal acetylation: from protein to physiology DOI Creative Commons
Nina McTiernan, Ine Kjosås, Thomas Arnesen

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 15, 2025

N-terminal acetylation is a highly abundant protein modification in eukaryotic cells. This catalysed by acetyltransferases acting co- or post-translationally. Here, we review the machinery: enzymes involved and their substrate specificities. We also provide an overview of impact acetylation, including its effects on folding, subcellular targeting, complex formation, turnover. In particular, there may be competition between other defining fate. At organismal level, influential, impairment was recently linked to cardiac dysfunction neurodegenerative diseases. ubiquitous eukaryotes. authors machinery, function fate, role physiology human disease.

Language: Английский

Citations

1
Protein N-terminal modifications: molecular machineries and biological implications DOI Creative Commons
Hanne Øye,

Malin Lundekvam,

Alessia Caiella

et al.

Trends in Biochemical Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The majority of eukaryotic proteins undergo N-terminal (Nt) modifications facilitated by various enzymes. These enzymes, which target the initial amino acid a polypeptide in sequence-dependent manner, encompass peptidases, transferases, cysteine oxygenases, and ligases. Nt - such as acetylation, fatty acylations, methylation, arginylation, oxidation enhance proteome complexity regulate protein targeting, stability, complex formation. Modifications at N termini are thereby core components large number biological processes, including cell signaling motility, autophagy regulation, plant animal oxygen sensing. Dysregulation Nt-modifying enzymes is implicated several human diseases. In this feature review we provide an overview occurring either co- or post-translationally, involved, impact.

Language: Английский

Citations

1
The nascent polypeptide-associated complex (NAC) as regulatory hub on ribosomes DOI
Laurenz Rabl, Elke Deuerling

Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Abstract The correct synthesis of new proteins is essential for maintaining a functional proteome and cell viability. This process tightly regulated, with ribosomes associated protein biogenesis factors ensuring proper production, modification, targeting. In eukaryotes, the conserved nascent polypeptide-associated complex (NAC) plays central role in coordinating early processing by regulating ribosome access multiple factors. NAC recruits modifying enzymes to ribosomal exit site N-terminus directs secretory into SRP-mediated targeting pathway. this review we will focus on these pathways, which are critical summarize recent advances understanding cotranslational functions mechanisms higher eukaryotes.

Language: Английский

Citations

0
A repository of Ogden syndrome (NAA10) and NAA15 patient derived iPSC lines and isogenic pairs by X-chromosome screening and genome-editing. DOI Creative Commons

Josephine Wesely,

Tom Rusielewicz,

Yu-Ren Chen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 28, 2024

Amino-terminal (Nt-) acetylation (NTA) is a common protein modification, affecting 80% of cytosolic proteins in humans. The human essential gene,

Language: Английский

Citations

0
Destined for destruction: The role of methionine aminopeptidases and plant cysteine oxidases in N-degron formation DOI Creative Commons
Andrea Fuentes-Terrón, Rebecca Latter, Samuel Madden

et al.

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 197(1)

Published: Dec. 23, 2024

Abstract The cysteine/arginine (Cys/Arg) branch of the N-degron pathway controls stability certain proteins with methionine (Met)-Cys N-termini, initiated by Met cleavage and Cys oxidation. In seeding plants, target include Group VII Ethylene Response Factors, which initiate adaptive responses to low oxygen (hypoxic) stress, as well Vernalization 2 (VRN2) Little Zipper (ZPR2), are involved in endogenous developmental hypoxia. It is essential that these only degraded under appropriate physiological conditions. Modification their N-termini enzymatic control Aminopeptidases (MetAPs) Plant Cysteine Oxidases (PCOs); therefore, substrate-binding requirements catalytic effectiveness enzymes important for defining Met-Cys–initiating degraded. Physiological conditions can also impact activity enzymes, well-characterized sensitivity PCOs ensures stabilized this review we compile functional structural properties MetAPs PCOs, including interactions substrates. We consider evolution through plant kingdom highlight role controlling initial steps pathway.

Language: Английский

Citations

0