Molecular requirements for C. elegans transgenerational epigenetic inheritance of pathogen avoidance DOI Creative Commons
Rachel Kaletsky, Rebecca S. Moore, Titas Sengupta

et al.

eLife, Journal Year: 2025, Volume and Issue: 14

Published: May 2, 2025

Bacteria are Caenorhabditis elegans’ food, and worms naturally attracted to many bacteria, including pathogenic Pseudomonas , preferring PA14 over laboratory Escherichia coli (OP50). Despite this natural attraction PA14, prior exposure causes the instead avoid PA14. This behavioral switch can happen quickly – even within duration of choice assay. We show that accurate assessment animals’ true first requires use a paralytic (azide) trap at their initial choice, preventing from avoidance assay period. previously discovered C. elegans 25°C plate-grown 20°C for 24 hr not only leads avoidance, but also four generations naïve progeny avoiding while other paradigms cause P0 and/or F1 avoidance. showed transgenerational (P0-F4) epigenetic is mediated by P11, small RNA produced P11 both necessary sufficient TEI learned highly expressed in our standard growth conditions (25°C on surfaces), conditions, suggesting reported failure observe F2-F4 likely due absence expression experimenters’ conditions. Additionally, we tested ~35 genes involvement pathogen The conservation multiple components sRNA mechanism across strains species suggests behavior be physiologically important wild

Language: Английский

Perception of a pathogenic signature initiates intergenerational protection DOI
Corinne L. Pender, Julian G. Dishart, Holly K. Gildea

et al.

Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Citations

5
Molecular Requirements for C. elegans Transgenerational Epigenetic Inheritance of Pathogen Avoidance DOI Creative Commons
Rachel Kaletsky, Rebecca S. Moore, Titas Sengupta

et al.

eLife, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

C. elegans’ major food source is bacteria, and worms are naturally attracted to many bacterial species, including pathogenic Pseudomonas ; in fact, prefer PA14 as well wild bacteria over the lab E. coli strain (OP50) standardly used laboratory setting. Many labs have shown that despite this natural attraction PA14, prior exposure causes instead avoid PA14. This behavioral switch can happen on a relatively fast time scale, even within duration of choice assay. Here we show accurate assessment animals’ true first requires use paralytic (azide) trap at their initial choice, prevent from avoidance assay period. We previously discovered elegans 25°C plate-grown 20°C for 24hrs not only leads these animals switching but also progeny avoiding naïve state, persists through F4 generation. Other types training cause P0 and/or F1 avoidance, do induce transgenerational (F2 beyond) inheritance. showed (P0-F4) learned mediated by P11, small RNA produced P11 both necessary sufficient epigenetic inheritance behavior. highly expressed our standard growth conditions (25°C surfaces), other conditions, suggesting reported failure observe F2-F4 most likely due absence expression experimenters’ conditions. Through mutant analyses, tested genes – germline regulators, uptake, interference/processing, chromatin modifiers, neuronal - involvement pathogen allowing us better understand molecular requirements process. found strains TEI least two P. vranovensis fluorescens 15, avoidance. The induced each species functions specific, distinct (Pv1 Pfs1 15 , respectively) either directly or indirectly reduce levels gene maco-1 which turn regulates daf-7 ASI neuron subsequent conservation multiple components mechanism across suggests behavior be functional physiologically important

Language: Английский

Citations

0
Molecular Requirements for C. elegans Transgenerational Epigenetic Inheritance of Pathogen Avoidance DOI Open Access
Rachel Kaletsky, Rebecca S. Moore, Titas Sengupta

et al.

Published: April 1, 2025

C. elegans’ major food source is bacteria, and worms are naturally attracted to many bacterial species, including pathogenic Pseudomonas ; in fact, prefer PA14 as well wild bacteria over the lab E. coli strain (OP50) standardly used laboratory setting. Many labs have shown that despite this natural attraction PA14, prior exposure causes instead avoid PA14. This behavioral switch can happen on a relatively fast time scale, even within duration of choice assay. Here we show accurate assessment animals’ true first requires use paralytic (azide) trap at their initial choice, prevent from avoidance assay period. We previously discovered elegans 25°C plate-grown 20°C for 24hrs not only leads these animals switching but also progeny avoiding naïve state, persists through F4 generation. Other types training cause P0 and/or F1 avoidance, do induce transgenerational (F2 beyond) inheritance. showed (P0-F4) learned mediated by P11, small RNA produced P11 both necessary sufficient epigenetic inheritance behavior. highly expressed our standard growth conditions (25°C surfaces), other conditions, suggesting reported failure observe F2-F4 most likely due absence expression experimenters’ conditions. Through mutant analyses, tested genes – germline regulators, uptake, interference/processing, chromatin modifiers, neuronal - involvement pathogen allowing us better understand molecular requirements process. found strains TEI least two P. vranovensis fluorescens 15, avoidance. The induced each species functions specific, distinct (Pv1 Pfs1 15 , respectively) either directly or indirectly reduce levels gene maco-1 which turn regulates daf-7 ASI neuron subsequent conservation multiple components mechanism across suggests behavior be functional physiologically important

Language: Английский

Citations

0
Unlocking the Potential of Curcumae Rhizoma Aqueous Extract in Stress Resistance and Extending Lifespan in Caenorhabditis elegans DOI Creative Commons

Linyao Jing,

Yanlin Zhao, Lijun Jiang

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(8), P. 1668 - 1668

Published: April 8, 2025

The enhancement of stress resistance is crucial for delaying aging and extending a healthy lifespan. Traditional Chinese medicine (TCM), cherished treasure heritage, has shown potential in mitigating promoting longevity. This study integrates network pharmacology vivo analysis to investigate the mechanisms effects Curcumae Rhizoma (C. Rhizoma), known as "E Zhu" Chinese. Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) identified 10 active compounds its aqueous extract, interacting with 128 stress-related targets. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) analyses revealed pathways such response, FoxO signaling, insulin resistance. In Caenorhabditis elegans, mg/mL C. extract improved UV, thermal, oxidative, pathogen-induced stress, lifespan dose-dependent manner. Mechanistically, it reduced reactive oxygen species (ROS), increased superoxide dismutase (SOD) activity, enhanced UV via insulin/IGF-1 pathway DAF-16 translocation. Molecular docking highlighted hexahydrocurcumin (HHC) related key bioactives. Furthermore, we also observed that significantly extended both healthspan nematodes. These findings highlight mitigation longevity promotion, offering valuable insights into therapeutic applications TCM.

Language: Английский

Citations

0
Behavioral adaptations of Caenorhabditis elegans against pathogenic threats DOI Creative Commons
Xin Zhao, Xinyu Li, Jiayi Gao

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19294 - e19294

Published: April 14, 2025

This review examines the behavioral adaptation mechanisms of Caenorhabditis elegans in response to pathogenic bacterial threats, emphasizing their ecological significance. It systematically explores how such as avoidance behavior, transgenerational learning, and forgetting enable C. optimize its survival reproductive strategies within dynamic microbial environments. detects harmful signals through chemosensation initiates behaviors. Simultaneously, it manages environmental energy allocation memory forgetting, allowing cope with selective pressures from fluctuations. In contrast, bacteria Pseudomonas aeruginosa Salmonella influence behavior toxin release biofilm formation, highlighting complex co-evolutionary dynamics between hosts pathogens. Additionally, these pathogens employ “Trojan Horse-like” “Worm Star” kill , further complicating host-pathogen interactions. These processes are driven by adaptations, biochemical signaling, evolutionary pressures, which emphasize niche ecosystems. serves a valuable model for studying study provides crucial theoretical insights into adaptive evolution ecosystem dynamics, offering guidance development biocontrol effective management

Language: Английский

Citations

0
Advancements in CRISPR-Cas-Based Strategies for Combating Antimicrobial Resistance DOI
Ruiting Zhang, Qi Zhou,

Siying Huang

et al.

Microbiological Research, Journal Year: 2025, Volume and Issue: unknown, P. 128232 - 128232

Published: May 1, 2025

Language: Английский

Citations

0
Biodegradable hydrogels and microbial consortia as a treatment for soil dysbiosis DOI Creative Commons
Roger B. Davis, Korena K Mafune, Mari K.H. Winkler

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 1, 2025

Terrestrial microbial communities drive many soil processes and can be pushed into a state of dysbiosis upon disturbance. This dysregulation negatively impacts biogeochemical cycles, which threatens plant health. Effective treatment requires simultaneous restoration multiple system components, addressing both the physical structure its communities. Hydrogels with consortia simultaneously remedy hydrodynamics while promoting reestablishment. The purpose this review is to shed light on management practices through lens dysbiosis. important address not only for health crop productivity, but also mitigate climate change improved carbon sequestration reduced greenhouse gas emissions. positions hydrogels microbes as tools dysbiosis, contributing agricultural resilience.

Language: Английский

Citations

0
Molecular requirements for C. elegans transgenerational epigenetic inheritance of pathogen avoidance DOI Creative Commons
Rachel Kaletsky, Rebecca S. Moore, Titas Sengupta

et al.

eLife, Journal Year: 2025, Volume and Issue: 14

Published: May 2, 2025

Bacteria are Caenorhabditis elegans’ food, and worms naturally attracted to many bacteria, including pathogenic Pseudomonas , preferring PA14 over laboratory Escherichia coli (OP50). Despite this natural attraction PA14, prior exposure causes the instead avoid PA14. This behavioral switch can happen quickly – even within duration of choice assay. We show that accurate assessment animals’ true first requires use a paralytic (azide) trap at their initial choice, preventing from avoidance assay period. previously discovered C. elegans 25°C plate-grown 20°C for 24 hr not only leads avoidance, but also four generations naïve progeny avoiding while other paradigms cause P0 and/or F1 avoidance. showed transgenerational (P0-F4) epigenetic is mediated by P11, small RNA produced P11 both necessary sufficient TEI learned highly expressed in our standard growth conditions (25°C on surfaces), conditions, suggesting reported failure observe F2-F4 likely due absence expression experimenters’ conditions. Additionally, we tested ~35 genes involvement pathogen The conservation multiple components sRNA mechanism across strains species suggests behavior be physiologically important wild

Language: Английский

Citations

0