Oral microbiota in preschoolers with rampant caries: a matched case–control study DOI Creative Commons
Yao Wu, Gui-Ding Li, Congmeng Lyu

et al.

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: Dec. 1, 2024

Abstract Rampant caries is identified by rapid onset, severe decay affecting multiple surfaces, and early pulp infection. This case–control study was conducted to investigate the disparities in oral microbiota between children affected rampant their caries-free counterparts. A total of 88 preschool children, with matched distribution sex age both case control groups, participated this study. Children’s health–related behaviors were reported parents, salivary pH levels assessed using a portable pen-type meter, supragingival dental plaque analyzed 16S rRNA gene sequencing. Children exhibited lower levels, poorer toothbrushing habits, more frequent consumption sugary snacks. Veillonella , enriched showed positive correlation negative candy consumption. Conversely, Fusobacterium Neisseria abundant caries, positively correlated frequency Furthermore, Streptococcus mutans Porphyromonas gingivalis Bacteroides acidifaciens as potential microbiome markers for differentiating preschoolers from peers. B. typically found gut, has been rarely field health. More well-designed cohort studies are recommended elucidate mechanisms through which gut influences pediatric patients offer insights into effective strategies management young children. Key points • Lower caries. Biomarkers predicting Impact on microbiota.

Language: Английский

Maternal milk fat globule membrane enriched gut L. murinus and circulating SCFAs to improve placental efficiency and fetal development in intrauterine growth restricted mice model DOI Creative Commons
Cuiping Feng, Yujun Wu, Xiangyu Zhang

et al.

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 6, 2025

Intrauterine growth restriction (IUGR) caused by placental dysfunctions leads to fetal defects. Maternal microbiome and its metabolites have been reported promote development. Milk fat globule membrane (MFGM) is known for diverse bioactive functions, while the effects of gestational MFGM supplementation on maternal gut microbiota, efficiency, development remained unclear. In this study, low protein diet-induced IUGR decreased litter birth weight, fetal/placental ratio in pregnant mice, restored these impairments. Meanwhile, during gestation enriched intestinal Lactobacillus murinus (L. murinus) increased luminal circulating short chain fatty acids (SCFAs) which improved efficiency due an enhanced antioxidant capacity a inflammation. addition, fecal microbiota transplantation (FMT) with MFGM-derived reprinted promoted phenotypes characteristics, L. enrichment, MFGM-fed were also recapitulated exogenous administration or SCFAs cocktail. Mechanically, MFGM, murinus, cocktail activated IUGR-induced depressive phosphorylation PI3K-Akt signaling placenta. Moreover, vitro cells cultivation under amino acid shortage model (AAS) oxygen-glucose (OGS) was used validate that key microbial cocktails can alleviate oxidative stress inflammation via activating PI3K/Akt signaling. Taken together, thereby improving growth, functions fetus. Our findings will provide theoretical support application maternal–placental-fetal nutrition address pregnancy malnutrition-induced IUGR.

Language: Английский

Citations

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Persimmon peel polysaccharides alleviated colitis via regulating gut microbiota and protecting intestinal barrier integrity DOI
Yanlong Cui, Shiqi Zhang, Xin Wang

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106123 - 106123

Published: Feb. 1, 2025

Language: Английский

Citations

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Yinzhihuang formula modulates the microbe‒gut‒liver axis and bile acid excretion to attenuate cholestatic liver injury DOI Creative Commons
Xin Luo, Peng Cheng, Yuan Fang

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 139, P. 156495 - 156495

Published: Feb. 16, 2025

Language: Английский

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Recent insights and advances in gut microbiota's influence on host antiviral immunity DOI Creative Commons
Ying Liu,

Danying Yan,

Ran Chen

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 27, 2025

A diverse array of microbial organisms colonizes the human body, collectively known as symbiotic communities. Among various pathogen infections that hosts encounter, viral represent one most significant public health challenges worldwide. The gut microbiota is considered an important biological barrier against and may serve a promising target for adjuvant antiviral therapy. However, potential impact on infection remains relatively understudied. In this review, we discuss specific regulatory mechanisms in immunity, highlighting recent advances how regulate host immune response, produce immune-related molecules, enhance host's defense viruses. Finally, also oral probiotics.

Language: Английский

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In vitro fermentation of Auricularia auricula polysaccharides and their regulation of human gut microbiota and metabolism DOI
Nannan Liu,

Sha Dai,

Xin Fan

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 306, P. 141714 - 141714

Published: March 4, 2025

Language: Английский

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Dietary Qiwenghuangbo powder–enriched Limosilactobacillus reuteri protects the intestinal epithelium and alleviates inflammation via a strain‐specific mechanism DOI Creative Commons
Qian Xu, Xue Lei, Zhenhua Wu

et al.

Animal Models and Experimental Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Abstract Background Qiwenghuangbo powder (QP), composed of Astragalus , Phellodendron chinensis and Radix pulsatilla is a traditional Chinese herbal formula, but its effects on weaned piglets remained unclear. Methods Weaned fed with 0.5 kg/t QP (QP1), 1 (QP2), low‐zinc oxide (ZnO; negative control), high‐ZnO (positive control) diets in two phases, respectively, the growth performance, intestinal morphology, cytokines, microbial communities were profiled. The mouse models colitis induced by Citrobacter rodentium dextran sulfate sodium (DSS) employed to elucidate potential role QP‐fed enriched key species. Results Dietary 1.0 alleviated diarrhea inflammation improved development performance piglets. Moreover, this dietary intervention notably altered microbiota composition, characterized enrichment Limosilactobacillus reuteri . Furthermore, out three isolated L. strains could alleviate pathogen infection inflammation, respectively. Specifically, anti‐inflammatory effect one strain was achieved promoting colonization resistance C. as significantly reduced loads. other mitigated DSS‐induced enhancing goblet cell function inhibiting secretion pro‐inflammatory particularly interleukin‐1β (IL‐1ß) tumor necrosis factor‐α (TNF‐α). Conclusions health commensal control strain‐specific mechanism, highlighting providing evidence for gut promotion.

Language: Английский

Citations

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Galacto-oligosaccharides alleviate experimental lactose intolerance associated with gut microbiota in mice DOI Creative Commons

Qianxi Li,

Xinlei Wang,

Siyu Guo

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 25, 2025

Introduction Galacto-oligosaccharides (GOS) are beneficial for alleviating lactose intolerance (LI). GOS have the ability to modify composition of intestinal microbiota. The development diseases could be influenced by gut Nevertheless, it remains unclear whether microbiota exerts an effect when alleviate LI, alterations in influence inflammatory response and digestion. Methods We first investigated effects on mice with established intolerance. Next, we demonstrated that prophylactic supplementation also conferred similar benefits. Results results showed enhanced anti-inflammatory, antioxidant, barrier function. observed mediated a change microbiome increasing abundance Lactobacillus . pre-supplementation reduced incident function, markedly altered significantly enriching Bifidobacterium Collectively, alleviation LI suggests intimate involvement probiotics. Discussion This study demonstrates ameliorated microbiota-dependent manner. Our findings provide novel evidence substitute lactase serve as potential modulator prevention LI.

Language: Английский

Citations

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3'-Sialyllactose and B. infantis synergistically alleviate gut inflammation and barrier dysfunction by enriching cross-feeding bacteria for short-chain fatty acid biosynthesis DOI Creative Commons

Mingzhi Yang,

Zipeng Jiang, Lin Zhou

et al.

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: April 7, 2025

Ulcerative colitis (UC) poses significant threats to human health and quality of life worldwide, as it is a chronic inflammatory bowel disease. 3'-sialyllactose (3'-SL) key functional component milk oligosaccharides. This study systematically evaluates the prebiotic effects 3'-SL its therapeutic potential in combination with Bifidobacterium infantis (B. infantis) for UC. The findings reveal that B. synergistically mitigate intestinal inflammation barrier dysfunction by promoting production short-chain fatty acids (SCFAs) through cross-feeding mechanisms among gut microbiota. Individually, 3'-SL, infantis, synbiotic treatment all effectively alleviated UC symptoms, including reduced weight loss, improved disease activity scores, prevention colon shortening. Histopathological immunofluorescence analyses further demonstrated significantly ameliorated colonic injury, enhanced function, restored goblet cell counts, increased glycoprotein content crypt cells, upregulated expression tight junction proteins (ZO-1, occludin, claudin-1). Notably, outperformed individual components better restoring microbiota balance, elevating SCFA levels, modulating serum cytokine profiles, thereby reducing inflammation. These provide mechanistic insights into protective underscore other disorders.

Language: Английский

Citations

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Baseline abundance of Akkermansia muciniphila and Bacteroides acidifaciens in a healthy state predicts inflammation associated tumorigenesis in the AOM/DSS mouse model DOI Creative Commons
Kseniya M. Achasova, Olga A. Snytnikova,

Kseniya E Chanushkina

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 10, 2025

Numerous studies demonstrate that intestinal microbiota contribute to colorectal cancer (CRC), which is often associated with dysbiosis. Most of the data were obtained from on CRC patients, making it challenging determine whether alterations in are a consequence pathology or they actively drive its progression. Several using laboratory animals suggest gut may be involved both onset and progression CRC. In present study we utilized azoxymethane-dextran sulfate sodium (AOM/DSS) mouse model investigate contribution healthy-state inflammation-associated tumorigenesis. Two cohorts C57BL/6 mice harboring different demonstrated susceptibility AOM/DSS treatment. Sequencing 16S rRNA bacterial DNA fecal samples revealed Akkermansia muciniphila Bacteroides acidifaciens as marker features (before administration), showed strong positive correlation tumor incidence. Moreover, abundance these markers, considered beneficial bacteria, was strongly positively correlated sulfate-reducing bacteria Desulfovibrio fairfieldensis identified chronic colitis-associated microbiota. Furthermore, abundances features, outcome, expression interferon gamma nitric oxide synthase 2 genes colon tissue during early stage DSS-induced inflammation. contrast multiple demonstrating anti-inflammatory properties A. B. acidifaciens, our results point out their potential adverse effect under specific conditions genotoxicity inflammation intestine. Taken together, findings complex, context-dependent role commensal dysbiosis

Language: Английский

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Unraveling the protective mechanisms and bioactive components of litchi polysaccharides in intestinal health DOI
Danmeng Luo,

Ruina Xu,

Jiang Li

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143383 - 143383

Published: April 1, 2025

Language: Английский

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