Conjugated Oligomer‐Based NIR‐II Chemiluminescence Nanosensor for In Vivo Imaging DOI
Ling Li, Xinyi Zhang, Yuxin Ren

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Abstract Chemiluminescence imaging has become a promising optical method because of its advantages such as no excitation source and minimal background autofluorescence. However, chemiluminescent nanosensors with second near‐infrared (NIR‐II) window emission are rarely reported. Here, NIR‐II chemiluminescence nanosensor OLBB‐CLS is designed by adopting the CRET (chemiluminescence resonance energy transfer) – FRET (fluorescent combined strategy, which consists OFBTL, BTD540‐C 8 , BBTD725‐C DSPE‐PEG 2000 . conjugated oligomer D‐A‐D (donor‐acceptor‐donor) molecular structure, acts probe 1 O 2 donor CRET. an receptor FRET, can absorb transfer to molecule thus producing fluorescence. Notably, there great overlap between spectrum OFBTL absorption enabling efficient them. Also, excellent occurs allowing effective FRET. The demonstrates remarkable biosafety achieve in vivo for ROS‐related disease high signal‐to‐noise ratio. This study paves way design probes imaging.

Language: Английский

Persistent Chemiluminescence-Enabled Digital Bead Counting for Quantifying Attomolar MicroRNAs DOI
Chao Lei,

Wenjiao Fan,

Jingjing Shi

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Digital biosensing is the state-of-the-art technique for precisely quantifying low-abundance biomarkers but heavily limited to sophisticated fabrication of sealed microchambers and fluorescence signal readout. Herein, a novel persistent, enhanced-chemiluminescence (E-CL)-enabled microchamber-free digital counting strategy proposed miRNA analysis by using fully open microbeads (MBs) as independent microreactors signaling units. The employment phenothiazine derivative enhancer efficiently transfers flash-type CL horseradish peroxidase (HRP)-H2O2-luminol into persistent stable E-CL with more than 103-fold enhancement. More importantly, leveraging single molecule-activated deposition HRP, driving power E-CL, on miRNA-loaded MBs, long-lasting can be finely sustained MBs' surface, achieving CL-based binary MB quantification at aM level. This E-CL-powered design may well complement prevalent fluorescence-based bioassays effectively addressing their inherent drawbacks (photobleaching/quenching scattering light background), thus expanding toolbox.

Language: Английский

Citations

0
Conjugated Oligomer‐Based NIR‐II Chemiluminescence Nanosensor for In Vivo Imaging DOI
Ling Li, Xinyi Zhang, Yuxin Ren

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Abstract Chemiluminescence imaging has become a promising optical method because of its advantages such as no excitation source and minimal background autofluorescence. However, chemiluminescent nanosensors with second near‐infrared (NIR‐II) window emission are rarely reported. Here, NIR‐II chemiluminescence nanosensor OLBB‐CLS is designed by adopting the CRET (chemiluminescence resonance energy transfer) – FRET (fluorescent combined strategy, which consists OFBTL, BTD540‐C 8 , BBTD725‐C DSPE‐PEG 2000 . conjugated oligomer D‐A‐D (donor‐acceptor‐donor) molecular structure, acts probe 1 O 2 donor CRET. an receptor FRET, can absorb transfer to molecule thus producing fluorescence. Notably, there great overlap between spectrum OFBTL absorption enabling efficient them. Also, excellent occurs allowing effective FRET. The demonstrates remarkable biosafety achieve in vivo for ROS‐related disease high signal‐to‐noise ratio. This study paves way design probes imaging.

Language: Английский

Citations

0