Genome-Wide Identification and Characterization of the mTERF Gene Family in Spinach and the Role of SomTERF5 in Response to Heat Stress DOI Creative Commons

Ziyue Sun,

Li Li, Yaqi Liu

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(11), P. 1570 - 1570

Published: May 22, 2025

Spinach (Spinacia oleracea L.), a globally consumed, nutrient-dense vegetable, contains diverse vitamins and minerals. However, elevated temperatures can constrain yield by interrupting leaf development photosynthetic efficiency. The mitochondrial transcription termination factor (mTERF) family, which regulates organellar gene expression, plays crucial roles in plant growth regulation. Thus, characterization of the spinach mTERF (SomTERF) family is critical for elucidating thermotolerance mechanisms this crop. In study, we systematically identified 31 SomTERF genes from genome, are distributed across five chromosomes nine unassembled genomic scaffolds. Subcellular localization predictions indicated that these proteins predominantly target chloroplasts mitochondria. Conserved domain analyses confirmed all possess canonical domains ten conserved motifs. Phylogenetic clustering segregated into distinct subgroups (I–IX), with significant divergence observed copy numbers among subgroups. Cis-element screening an abundance heat-, cold-, hormone-responsive motifs within promoter regions. Notably, seven members (including SomTERF5) exhibited pronounced enrichment heat shock elements (HSEs). Organ-specific expression profiling revealed preferential genes. Comparative RT-qPCR heat-sensitive (Sp73) heat-tolerant (Sp75) cultivars under thermal stress demonstrated genotype-dependent dynamics. Functional validation SomTERF5 was achieved through cloning, transgenic Arabidopsis overexpressing showed significantly enhanced thermotolerance, as evidenced improved survival rates following treatment. Yeast two-hybrid (Y2H) assays further physical interaction between SopTAC2. This study provides comprehensive foundation understanding mTERF-mediated developmental regulation advanced molecular breeding strategies developing heat-resilient varieties.

Language: Английский

Genome-Wide Identification and Characterization of the mTERF Gene Family in Spinach and the Role of SomTERF5 in Response to Heat Stress DOI Creative Commons

Ziyue Sun,

Li Li, Yaqi Liu

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(11), P. 1570 - 1570

Published: May 22, 2025

Spinach (Spinacia oleracea L.), a globally consumed, nutrient-dense vegetable, contains diverse vitamins and minerals. However, elevated temperatures can constrain yield by interrupting leaf development photosynthetic efficiency. The mitochondrial transcription termination factor (mTERF) family, which regulates organellar gene expression, plays crucial roles in plant growth regulation. Thus, characterization of the spinach mTERF (SomTERF) family is critical for elucidating thermotolerance mechanisms this crop. In study, we systematically identified 31 SomTERF genes from genome, are distributed across five chromosomes nine unassembled genomic scaffolds. Subcellular localization predictions indicated that these proteins predominantly target chloroplasts mitochondria. Conserved domain analyses confirmed all possess canonical domains ten conserved motifs. Phylogenetic clustering segregated into distinct subgroups (I–IX), with significant divergence observed copy numbers among subgroups. Cis-element screening an abundance heat-, cold-, hormone-responsive motifs within promoter regions. Notably, seven members (including SomTERF5) exhibited pronounced enrichment heat shock elements (HSEs). Organ-specific expression profiling revealed preferential genes. Comparative RT-qPCR heat-sensitive (Sp73) heat-tolerant (Sp75) cultivars under thermal stress demonstrated genotype-dependent dynamics. Functional validation SomTERF5 was achieved through cloning, transgenic Arabidopsis overexpressing showed significantly enhanced thermotolerance, as evidenced improved survival rates following treatment. Yeast two-hybrid (Y2H) assays further physical interaction between SopTAC2. This study provides comprehensive foundation understanding mTERF-mediated developmental regulation advanced molecular breeding strategies developing heat-resilient varieties.

Language: Английский

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