Antibody-guided identification of Achromobacter xylosoxidans protein antigens in cystic fibrosis DOI Creative Commons
Cecilia Sahl,

Sounak Chowdhury,

Johan Malmström

et al.

mSphere, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

ABSTRACT Persistent bacterial airway infection is a hallmark feature of cystic fibrosis (CF). Achromobacter spp. are gram-negative rods that can cause persistent in people with CF (pwCF), but the knowledge host immune responses to these bacteria limited. The aim this study was investigate if patients develop antibodies against xylosoxidans , most common species, and identify antigens induce specific IgG responses. Seven serum samples from pwCF were screened for an ELISA coated A. insuavis, or Pseudomonas aeruginosa . Sera without P. ( n = 22 20, respectively) healthy donors 4) included comparison. Serum high titers selected affinity purification using IgGs bound protein G beads. resulting IgG-antigen complexes then analyzed liquid chromatography-tandem mass spectrometry (LC-MS/MS). Selected interest produced recombinant form used confirm results. Four seven had Using patient serum-IgG proteins, we identified eight antigens. Three these, which not targeted by anti- P antibodies, expressed recombinantly further validation: dihydrolipoyl dehydrogenase (DLD), type I secretion C-terminal target domain-containing protein, domain uncharacterized function 336 (DUF336). While all three confirmed DLD DUF336 showed least binding infection. combination LC-MS/MS confirming results have IMPORTANCE species opportunistic pathogens infections fibrosis. In population, associated low lung function, about interactions currently study, antibody host. may potentially be useful serological assays, serving as complement culturing methods diagnosis surveillance

Language: Английский

Antibody-guided identification of Achromobacter xylosoxidans protein antigens in cystic fibrosis DOI Creative Commons
Cecilia Sahl,

Sounak Chowdhury,

Johan Malmström

et al.

mSphere, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

ABSTRACT Persistent bacterial airway infection is a hallmark feature of cystic fibrosis (CF). Achromobacter spp. are gram-negative rods that can cause persistent in people with CF (pwCF), but the knowledge host immune responses to these bacteria limited. The aim this study was investigate if patients develop antibodies against xylosoxidans , most common species, and identify antigens induce specific IgG responses. Seven serum samples from pwCF were screened for an ELISA coated A. insuavis, or Pseudomonas aeruginosa . Sera without P. ( n = 22 20, respectively) healthy donors 4) included comparison. Serum high titers selected affinity purification using IgGs bound protein G beads. resulting IgG-antigen complexes then analyzed liquid chromatography-tandem mass spectrometry (LC-MS/MS). Selected interest produced recombinant form used confirm results. Four seven had Using patient serum-IgG proteins, we identified eight antigens. Three these, which not targeted by anti- P antibodies, expressed recombinantly further validation: dihydrolipoyl dehydrogenase (DLD), type I secretion C-terminal target domain-containing protein, domain uncharacterized function 336 (DUF336). While all three confirmed DLD DUF336 showed least binding infection. combination LC-MS/MS confirming results have IMPORTANCE species opportunistic pathogens infections fibrosis. In population, associated low lung function, about interactions currently study, antibody host. may potentially be useful serological assays, serving as complement culturing methods diagnosis surveillance

Language: Английский

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