Fabrication of novel purine nucleoside derivatives bearing a cinnamic acid moiety for controlling plant viral diseases: synthesis, anti‐tobacco mosaic virus activity, and mechanistic study

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

In recent years, many plant viral diseases have triggered global epidemics, posing an intractable threat to food safety and the economy. However, antiviral agents currently remain insufficient due their limited control effect photothermal instability. Consequently, development of alternatives with high efficiency eco-friendly manifestation is a matter great urgency importance. Herein, 34 purine nucleoside derivatives bearing cinnamic acid building block were constructed underwent evaluation anti-tobacco mosaic virus (anti-TMV) potency. Biological assays demonstrated that compound A14 displayed excellent curative activity half maximal effective concentration (EC50) 274.25 mg L-1, which surpassed reference ribavirin (783.54 L-1) ningnanmycin (283.26 L-1). The mechanism action was further investigated through fluorescence confocal microscopy, reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR), TMV self-assembly, molecular docking, titration, transmission electron microscopy. Outcomes indicated could prevent systemic biosynthesis in plants, addition blocking self-assembly via interacting TMV-coat protein. Our research efforts resulted verification as novel agent alternative, possessed dual function inhibiting process TMV, for controlling diseases. © 2025 Society Chemical Industry.

Language: Английский

Berberine from Corydalis saxicola Bunting reveals anti-tomato spotted wilt orthotospovirus activity with a novel mechanism

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 110119 - 110119

Published: June 1, 2025

Language: Английский

The 6-kilodalton peptide 1 of the family Potyviridae: small in size but powerful in function

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: June 5, 2025

The Potyviridae family is one of the most economically significant groups plant RNA viruses, causing severe yield losses in agriculturally important crops. Among viral proteins encoded by potyviruses, 6-kilodalton peptide 1 (6K1) has emerged as a critical, albeit poorly understood player pathogenesis. Despite its small size, 6K1 exhibits diverse functions, including facilitating assembly replication complex (VRC), altering host membrane permeability viroporin, and interacting with factors to promote infection. This review synthesizes current knowledge on 6K1, focusing structural characteristics, evolutionary conservation, molecular interactions, potential target for antiviral strategies. We further discuss unresolved questions surrounding putative ion channel activity, polyprotein processing dynamics, functional parallels animal virus viroporins. Understanding 6K1’s multifunctionality provides new insights into infection mechanisms opens avenues novel disease control approaches.

Language: Английский