An improved plant prime editor for efficient generation of multiple-nucleotide variations and structural variations in rice

Plant Communications, Journal Year: 2024, Volume and Issue: 5(9), P. 100976 - 100976

Published: May 14, 2024

Language: Английский

---

Advances in cis-element- and natural variation-mediated transcriptional regulation and applications in gene editing of major crops

Journal of Experimental Botany, Journal Year: 2023, Volume and Issue: 74(18), P. 5441 - 5457

Published: June 29, 2023

Transcriptional regulation is crucial to control of gene expression. Both spatio-temporal expression patterns and levels genes are determined by the interaction between cis-acting elements trans-acting factors. Numerous studies have focused on factors that mediate transcriptional regulatory networks. However, elements, such as enhancers, silencers, transposons, natural variations in genome, also vital for could be utilized clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated editing improve crop quality yield. In this review, we discuss current understanding cis-element-mediated major crops, including rice (Oryza sativa), wheat (Triticum aestivum), maize (Zea mays), well latest advancements techniques their applications crops highlight prospective strategies breeding.

Language: Английский

---

Efficient genome editing in grapevine using CRISPR/LbCas12a system

Molecular Horticulture, Journal Year: 2023, Volume and Issue: 3(1)

Published: Oct. 18, 2023

Clustered regularly interspaced short palindromic repeats (CRISPR) /Cas12a system, also known as CRISPR/Cpf1, has been successfully harnessed for genome engineering in many plants, but not grapevine yet. Here we developed and demonstrated the efficacy of CRISPR/Cas12a from Lachnospiraceae bacterium ND2006 (LbCas12a) inducing targeted mutagenesis by targeting tonoplastic monosaccharide transporter1 (TMT1) dihydroflavonol-4-reductase 1 (DFR1) genes 41B cells. Knockout DFR1 gene altered flavonoid accumulation dfr1 mutant Heat treatment (34℃) improved editing efficiencies CRISPR/LbCas12a TMT1-crRNA1 TMT1-crRNA2 increased 35.3% to 44.6% 29.9% 37.3% after heat treatment, respectively. Moreover, sequences crRNAs were found be predominant factor affecting irrespective positions within crRNA array designed multiplex editing. In addition, with truncated (trucrRNAs) showed that trucrRNAs 20 nt guide effective original 24 guides generating mutagenesis, whereas shorter regions target complementarity ≤ 18 length may induce detectable mutations All these results provide evidence further applications system a powerful tool engineering.

Language: Английский

---

Integrating machine learning and genome editing for crop improvement

aBIOTECH, Journal Year: 2024, Volume and Issue: 5(2), P. 262 - 277

Published: Feb. 29, 2024

Genome editing is a promising technique that has been broadly utilized for basic gene function studies and trait improvements. Simultaneously, the exponential growth of computational power big data now promote application machine learning biological research. In this regard, shows great potential in refinement genome systems crop improvement. Here, we review advances to optimization, with emphasis placed on efficiency specificity enhancement. Additionally, demonstrate how bridges breeding, by accurate key site detection guide RNA design. Finally, discuss current challenges prospects these two techniques By integrating advanced learning, progress breeding will be further accelerated future.

Language: Английский

---

IsDge10 is a hypercompact TnpB nuclease that confers efficient genome editing in rice

Plant Communications, Journal Year: 2024, Volume and Issue: 5(11), P. 101068 - 101068

Published: Aug. 23, 2024

Language: Английский

---