Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 222, P. 119655 - 119655
Published: Sept. 14, 2024
Language: Английский
Molecular Plant, Journal Year: 2024, Volume and Issue: 17(3), P. 423 - 437
Published: Jan. 24, 2024
Nicotiana tabacum and benthamiana are widely used models in plant biology research. However, genomic studies of these species have lagged. Here we report the chromosome-level reference genome assemblies for N. with an estimated 99.5% 99.8% completeness, respectively. Sensitive transcription start termination site sequencing methods were developed accurate gene annotation tabacum. Comparative analyses revealed evidence parental origins chromosome structural changes, leading to hybrid formation each species. Interestingly, antiviral silencing genes RDR1, RDR6, DCL2, DCL3, AGO2 lost from one or both subgenomes benthamiana, while homeologs kept Furthermore, encodes fewer immune receptors signaling components than that These findings uncover possible reasons underlying hypersusceptible nature benthamiana. We user-friendly Nicomics (http://lifenglab.hzau.edu.cn/Nicomics/) web server facilitate better use resources as well structure expression analyses.
Language: Английский
Citations
33
Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)
Published: April 16, 2024
Abstract Nicotiana benthamiana is a fundamental model organism in plant research. Recent advancements genomic sequencing have revealed significant intraspecific genetic variations. This study addresses the pressing need for precise genome sequence specific to its geographic origin by presenting comprehensive assembly of N. LAB strain from Republic Korea (NbKLAB). We compare this with widely used NbLAB360 strain, shedding light on essential differences between them. The outcome high-quality, chromosome-level comprising 19 chromosomes, spanning 2,762 Mb, an N50 142.6 Mb. Comparative analyses notable variations, including 46,215 protein-coding genes, impressive 99.5% BUSCO completeness score. Furthermore, NbKLAB substantially improved QV 33% 49%. refined chromosomal , conjunction comparative insights, provides valuable resource genomics research and molecular biology. accomplishment forms strong foundation in-depth exploration into intricacies genetics genomics, precision, framework.
Language: Английский
Citations
11
Current Opinion in Plant Biology, Journal Year: 2024, Volume and Issue: 81, P. 102611 - 102611
Published: Aug. 3, 2024
Nicotiana benthamiana is a model plant, widely used for research. The susceptibility of young plants to Agrobacterium tumefaciens has been utilised transient gene expression, enabling the production recombinant proteins at laboratory and commercial scales. More recently, this technique rapid prototyping synthetic genetic circuits elucidation reconstruction metabolic pathways. In last few years, many complex pathways have successfully reconstructed in species. addition, availability improved genomic resources efficient editing tools enabled application sophisticated engineering approaches increase purity yield target compounds. review, we discuss recent advances use N. understanding plant metabolism, as well efforts improve utility species chassis natural products.
Language: Английский
Citations
11
Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 225, P. 120605 - 120605
Published: Jan. 29, 2025
Language: Английский
Citations
1
Trends in Biochemical Sciences, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 1, 2025
Language: Английский
Citations
1
Annual Review of Plant Biology, Journal Year: 2024, Volume and Issue: 75(1), P. 629 - 653
Published: March 1, 2024
Plant specialized metabolites (PSMs) are variably distributed across taxa, tissues, and ecological contexts; this variability has inspired many theories about PSM function, which, to date, remain poorly tested because predictions have outpaced the available data. Advances in mass spectrometry–based metabolomics enabled unbiased profiling, molecular biology techniques produced PSM-free plants; combination of these methods accelerated our understanding complex roles that PSMs play plants. Synthetic workflows producing high-value, structurally quantities purities sufficient for both medicinal functional studies. These enable reengineering transport, externalization, structural diversity, production novel facilitating rigorous tests long-standing theoretical why plants produce so different particular tissues contexts. Plants use their chemical prowess solve challenges, synthetic accelerating evolved functions.
Language: Английский
Citations
7
Nature Chemical Biology, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 13, 2024
Language: Английский
Citations
7
New Phytologist, Journal Year: 2024, Volume and Issue: 243(2), P. 674 - 687
Published: May 16, 2024
Synthetic biology has the potential to revolutionize biotechnology, public health, and agriculture. Recent studies have shown enormous of plants as chassis for synthetic applications. However, tools precisely manipulate metabolic pathways bioproduction in are still needed. We used bacterial allosteric transcription factors (aTFs) that control gene expression a ligand-specific manner tested their ability repress semi-synthetic promoters plants. also modulation repression activity response specific plant metabolites, especially phenylpropanoid-related molecules. Using these aTFs, we designed genetic circuits capable computing Boolean logic operations. Three CouR, FapR, TtgR, achieved c. 95% respective target promoters. For sixfold de-repression could be triggered by inducing its ligand accumulation, showing use biosensor. Moreover, AND, NAND, IMPLY, NIMPLY operations integrate metabolite levels input circuit. showed biosensors can implemented detect metabolites activate circuit follows predefined logic, demonstrating exerting over facilitating natural products.
Language: Английский
Citations
5
Nature Plants, Journal Year: 2024, Volume and Issue: 10(11), P. 1749 - 1760
Published: Oct. 11, 2024
Abstract Recognizing pathogen-associated molecular patterns on the cell surface is crucial for plant immunity. The proteinaceous nature of many these suggests that secreted proteases play important roles in their formation and stability. Here we demonstrate apoplastic subtilase SBT5.2a inactivates immunogenicity cold-shock proteins (CSPs) bacterial pathogen Pseudomonas syringae by cleaving within immunogenic csp22 epitope. Consequently, mutant plants lacking activity retain higher levels csp22, leading to enhanced immune responses reduced growth. SBT5.2 sensitivity influenced sequence variation surrounding cleavage site probably extends CSPs from other species. These findings suggest variations stability among pathogens are a factor plant–bacteria interactions exploit avoid pattern recognition.
Language: Английский
Citations
4
Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15
Published: Jan. 10, 2025
Kiwifruit (Actinidia deliciosa)-derived actinidin, a cysteine protease, is renowned for its meat-tenderizing and milk-clotting activities. Despite potential in various biotechnological applications, an efficient expression platform actinidin production has not yet been developed. Instead, traditionally purified directly from the fruits of plants. This study aimed to produce kiwifruit-derived leaves Nicotiana benthamiana. The expressed was directed lumen endoplasmic reticulum (ER) using binding immunoglobulin protein (BiP) signal sequence ER retention signal. To facilitate cost-effective purification, family 3 cellulose-binding module (CBM3) employed as affinity tag, along with microcrystalline cellulose beads that bind efficiently CBM3. A significant portion recovered soluble fraction without proteolytic degradation. exhibited β-casein-degrading activity, optimal efficiency observed at 55°C pH 7.0. These results establish promising plant-based functional application diverse processes.
Language: Английский
Citations
0