Harnessing transposable elements for plant functional genomics and genome engineering DOI Creative Commons
Xiaoyuan Tao, Siyu Feng, Yuan Lü

et al.

Trends in Plant Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Transposable elements (TEs) constitute a large portion of many plant genomes and play important roles in regulating gene expression driving genome evolution crop domestication. Despite advances understanding the functions mechanisms TEs, comprehensive review their integrated knowledge cutting-edge biotechnological applications TEs is still needed. We provide thorough overview that connects discoveries, mechanisms, technologies associated with TEs. discuss identification function driven by functional genomics, epigenetic regulation utilization active genomics engineering. In summary, expanding application will be beneficial to breeding synthetic biology future.

Language: Английский

GENOME EDITING FOR IMPROVING DISEASE RESISTANCE IN RICE (Oryza sativa L.) - SCOPE, ACHIEVEMENTS, LIMITATIONS AND FUTURE NEEDS DOI
Shobica Priya Ramasamy,

Sakthi Ambothi Rathnasamy,

Sudha Manickam

et al.

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102640 - 102640

Published: March 1, 2025

Language: Английский

Citations

0

CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops DOI Creative Commons
Navjot Kaur, Muslim Qadir, Dali Vilma Francis

et al.

Frontiers in Genome Editing, Journal Year: 2025, Volume and Issue: 7

Published: March 18, 2025

Climate change is a global concern for agriculture, food security, and human health. It affects several crops causes drastic losses in yield, leading to severe disturbances the economy, environment, community. The consequences on important staple crops, such as rice, maize, wheat, will worsen create insecurity across globe. Although various methods of trait improvements are available being used, clustered regularly interspaced short palindromic repeats CRISPR-associated protein 9 (CRISPR/Cas9) mediated genome manipulation have opened new avenue functional genomics crop improvement. This review discuss progression improvement from conventional breeding advanced editing techniques how CRISPR/Cas9 technology can be applied enhance tolerance main cereal (wheat, maize) against any harsh climates. CRISPR/Cas endonucleases their derived genetic engineering tools possess high accuracy, versatile, more specific, easy design, climate-smart or resilient combat survive environments. CRISPR/Cas9-mediated approach has been make them climate resilient. review, supported by bibliometric analysis recent literature, highlights potential target genes/traits addresses significance gene technologies tackling vulnerable effects major maize.

Language: Английский

Citations

0

Beyond a few bases: methods for large DNA insertion and gene targeting in plants DOI Creative Commons

Katie Vollen,

José M. Alonso, Anna N. Stepanova

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(6)

Published: March 1, 2025

SUMMARY Genome editing technologies like CRISPR/Cas have greatly accelerated the pace of both fundamental research and translational applications in agriculture. However, many plant biologists are functionally limited to creating small, targeted DNA changes or large, random insertions. The ability efficiently generate yet precise, will massively accelerate crop breeding cycles, enabling researchers more engineer crops amidst a rapidly changing agricultural landscape. This review provides an overview existing that allow integrate large sequences within host some associated technical bottlenecks. Additionally, this explores selection emerging techniques other systems inspire tool development plants.

Language: Английский

Citations

0

Harnessing transposable elements for plant functional genomics and genome engineering DOI Creative Commons
Xiaoyuan Tao, Siyu Feng, Yuan Lü

et al.

Trends in Plant Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Transposable elements (TEs) constitute a large portion of many plant genomes and play important roles in regulating gene expression driving genome evolution crop domestication. Despite advances understanding the functions mechanisms TEs, comprehensive review their integrated knowledge cutting-edge biotechnological applications TEs is still needed. We provide thorough overview that connects discoveries, mechanisms, technologies associated with TEs. discuss identification function driven by functional genomics, epigenetic regulation utilization active genomics engineering. In summary, expanding application will be beneficial to breeding synthetic biology future.

Language: Английский

Citations

0