Aging disrupts spatiotemporal coordination in the cycling ovary DOI Creative Commons
Tammy C. T. Lan, David S. Fischer, Alison Kochersberger

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Summary Throughout the female reproductive lifespan, ovary completes hundreds of cycles follicle development, ovulation, and tissue regeneration 1–3 . These processes rely on precise coordination intricate multicellular interactions across time space 4 How aging disrupts these interactions, leading to overall decline in endocrine functions, remains understudied. To understand dynamics that underlie ovarian function their changes with age, here we use Slide-seq, a near-cellular spatial transcriptomics method, profile 21 ovaries chronological representing 601,831 spots 68 transcriptomic profiles 5,6 We develop segmentation analysis identify static snapshots niches capture folliculogenesis situ , allowing us examine 345 oocytes, 653 follicles, 234 corpora lutea. find both organization temporal before cessation cycling, dysregulation hormone production signaling. disruptions are marked by altered immune cell dynamics, inflammatory signaling, global disorganization impair cyclic remodeling required for function. Our findings reveal how orchestrate functions demonstrate age-related breakdown precedes decline.

Language: Английский

Regulatory signatures involved in the cell cycle pathway contribute to egg production heterosis in chicken DOI Creative Commons

Jingwei Yuan,

Yuanmei Wang, Yanyan Sun

et al.

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 4, 2025

Abstract Background Crossbreeding is widely promoted as an efficient strategy to improve the productivity in agriculture. The molecular mechanism underlying heterosis for egg production always intriguing chicken. transcriptional dynamic changes play a crucial role formation of heterosis, but little known traits. Results In present study, we measured continuous manifestation ranging from 2.67% 10.24% number crossbreds generated by reciprocal crossing White Leghorn and Beijing You high-quality transcriptomes ovary purebreds (WW YY) (WY YW) 5 laying stages were sequenced integrated identify regulatory networks relevant heterosis. We found highly conserved features among 4 genetic groups. By using weighted gene co-expression network analysis (WGCNA), obtained multiple modules that significantly correlated with each group. common KEGG pathways including apelin signaling pathway, cell cycle, ribosome, spliceosome oxidative phosphorylation, screened 2 crossbreds. Then, identified consensus (CMs) showed divergent expression pattern crossbred or YY). hub genes CMs again overrepresented cycle exhibited temporally complementary dominance stages. These results suggested inherited both parents maintain function cycle-related genes, contributing persistent production. Furthermore, dominant MAD2L1 , CHEK2 E2F1 demonstrated ovarian follicle development maturation could be candidate Conclusion Our study characterized profile genome-wide highlighted pathway findings provided new insights which would facilitate rational choice suitable producing chickens higher

Language: Английский

Citations

0
Tetramethyl bisphenol F exposure induces human ovarian granulosa cell senescence and mouse ovarian damage by regulating ESRRB signalling DOI Creative Commons

Huiyu Fan,

Chunming Li, Yifei Lv

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 292, P. 117940 - 117940

Published: Feb. 27, 2025

Language: Английский

Citations

0
Senotherapeutic approach to age-related endocrine diseases DOI
Seda Turgut,

Gülnur Andican

Advances in pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Regulatory signatures involved in the cell cycle pathway contribute to egg production heterosis in chicken DOI

Jingwei Yuan,

Yuanmei Wang, Yanyan Sun

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 19, 2024

Abstract Background Crossbreeding is widely promoted as an efficient strategy to improve the productivity in agriculture. The molecular mechanism underlying heterosis for egg production always intriguing chicken. transcriptional dynamic changes play a crucial role formation of heterosis, but little known traits. Results In present study, we measured continuous manifestation ranging from 2.67–10.24% number crossbreds generated by reciprocal crossing White Leghorn and Beijing You high-quality transcriptomes ovary purebreds (WW YY) (WY YW) five laying stages were sequenced integrated identify regulatory networks relevant heterosis. We found highly conserved features among four genetic groups. By using weighted gene co-expression network analysis (WGCNA), firstly obtained multiple modules significantly correlated with each group. common KEGG pathways including apelin signaling pathway, cell cycle, ribosome, spliceosome oxidative phosphorylation, screened crossbreds. Then, identified consensus (CMs) that showed divergent expression pattern or YY). hub genes CMs again overrepresented cycle exhibited temporally complementary dominance stages. These suggested inherited both parents maintain function cycle-related genes, contributing persistent Furthermore, dominant MAD2L1, CHEK2 E2F1 demonstrated ovarian follicle development maturation could be candidate Conclusion Our study characterized profile genome-wide highlighted pathway findings provided new insights which would facilitate rational choice suitable producing crossbred chickens higher production.

Language: Английский

Citations

0
Effect of senolytic drugs in young female mice chemically induced to estropause DOI
Bianca M. Ávila, Bianka M. Zanini, Karina Pereira Luduvico

et al.

Life Sciences, Journal Year: 2024, Volume and Issue: unknown, P. 123073 - 123073

Published: Sept. 1, 2024

Language: Английский

Citations

0
p53 dependence of senescence markers p21v1 and p21v2 in aging and acute injury DOI Creative Commons
Parmita Kar,

Ashok Sivasailam,

Rupa Lavarti

et al.

npj Aging, Journal Year: 2024, Volume and Issue: 10(1)

Published: Oct. 14, 2024

The senescence phenotype is heterogeneous, as observed by the context-dependent differential expression of markers. Here, we provide evidence to demonstrate an inverse relationship in pattern two murine variants p21 (p21v1, and p21v2) aging hemorrhagic shock. While upregulation p21v1 was following shock injury, p21v2 upregulated aged mouse. We further show that response is, at least, partially independent p53.

Language: Английский

Citations

0
Protective effect of adiponectin on oxidative stress-induced ovarian granulosa cell senescence in geese DOI Creative Commons
Yan Zheng,

Yunqiao Qiu,

Ming Gao

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 104(1), P. 104529 - 104529

Published: Nov. 7, 2024

Geese are susceptible to oxidative stress during breeding, leading senescence of granulosa cells (GCs) and reduced egg production. Adiponectin (ADPN) is a cytokine secreted by adipose tissue that functions regulate metabolism antioxidants. However, its role in the regulation goose GCs unclear. To investigate this, primary was induced D-gal assessed via RT‒qPCR, senescence-associated β-galactosidase (SA-β-gal) staining, immunofluorescence, flow cytometry, transcriptomics. The effect ADPN on GC investigated overexpressing knocking down expression. results showed could alleviate cell cycle arrest GCs, reduce expression secretory phenotype (SASP)-related genes IL-6 IL-8, metabolic capacity accumulation SA-β-gal, maintain telomere length, D-gal. RNA-seq provided further evidence for regulatory senescence. shown attenuate stress-induced through AGE (Advanced glycation end products)-RAGE (Receptor advanced products) NOD-like receptor pathways. These findings may contribute development improved theoretical references improving egg-laying performance prolonging service life geese.

Language: Английский

Citations

0
Aging disrupts spatiotemporal coordination in the cycling ovary DOI Creative Commons
Tammy C. T. Lan, David S. Fischer, Alison Kochersberger

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Summary Throughout the female reproductive lifespan, ovary completes hundreds of cycles follicle development, ovulation, and tissue regeneration 1–3 . These processes rely on precise coordination intricate multicellular interactions across time space 4 How aging disrupts these interactions, leading to overall decline in endocrine functions, remains understudied. To understand dynamics that underlie ovarian function their changes with age, here we use Slide-seq, a near-cellular spatial transcriptomics method, profile 21 ovaries chronological representing 601,831 spots 68 transcriptomic profiles 5,6 We develop segmentation analysis identify static snapshots niches capture folliculogenesis situ , allowing us examine 345 oocytes, 653 follicles, 234 corpora lutea. find both organization temporal before cessation cycling, dysregulation hormone production signaling. disruptions are marked by altered immune cell dynamics, inflammatory signaling, global disorganization impair cyclic remodeling required for function. Our findings reveal how orchestrate functions demonstrate age-related breakdown precedes decline.

Language: Английский

Citations

0