Graph-based 3D spatial gene neighborhood networks of single cells in gels and tissues

BME Frontiers, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 1, 2025

Objective: We developed 3-dimensional spatially resolved gene neighborhood network embedding (3D-spaGNN-E) to find subcellular proximity relationships and identify key motifs in cell–cell communication (CCC). Impact Statement: The pipeline combines 3D imaging-based spatial transcriptomics graph-based deep learning motifs. Introduction: Advancements imaging experimental technology allow the study of capture better context than approximating samples as 2D. However, third dimension increases data complexity requires new analyses. Methods: 3D-spaGNN-E detects single transcripts cell culture identifies relationships. Then, a graph autoencoder projects into latent space. then applied explainability analysis CCC Results: first mesenchymal stem cells (MSCs) cultured hydrogel. After clustering based on RNA count, we identified belonging same cluster homotypic those different clusters heterotypic. changes local near border between heterotypic cells. When applying MSC–peripheral blood mononuclear (PBMC) coculture system, CD4 + CD8 T Local can distinguish strong weak suppression immune Lastly, compared astrocyte–neuron mouse hypothalamus cortex by analyzing multiplexed-error-robust fluorescence situ hybridization (MERFISH) regional differences. Conclusion: distinguished distinct CCCs tissue examining

Language: Английский

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Transformative Advances in Modeling Brain Aging and Longevity: Success, Challenges and Future Directions

Ageing Research Reviews, Journal Year: 2025, Volume and Issue: unknown, P. 102753 - 102753

Published: April 1, 2025

Language: Английский

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Emerging Trends in Microfluidic Biomaterials: From Functional Design to Applications

Journal of Functional Biomaterials, Journal Year: 2025, Volume and Issue: 16(5), P. 166 - 166

Published: May 8, 2025

The rapid development of microfluidics has driven innovations in material engineering, particularly through its ability to precisely manipulate fluids and cells at microscopic scales. Microfluidic biomaterials, a cutting-edge interdisciplinary field integrating microfluidic technology with biomaterials science, are revolutionizing biomedical research. This review focuses on the functional design fabrication organ-on-a-chip (OoAC) platforms via 3D bioprinting, explores applications drug delivery, cell culture, tissue evaluates potential systems advancing personalized healthcare. We systematically analyze evolution materials—from silicon glass polymers paper—and highlight advantages bioprinting over traditional methods. Currently, despite significant advances medicine, challenges scalability, stability, clinical translation remain. future will depend combining dynamic design, developing hybrid strategies that combine molds bio-printed structures, using artificial intelligence monitor delivery or response real time. believe collaborations between materials micromachining, medicine accelerate into therapies high-throughput screening tools.

Language: Английский

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Actionable Health Investments for Longevity: Empowering Consumers to Shape the Healthcare Ecosystem

Published: May 13, 2025

This commentary explores the rise of proactive health management and its implications for consumer behavior, healthcare systems, long-term outcomes. It argues that personalized, preventive care—via digital tools, diagnostics, supplementation—can be cost-effective when compared to routine discretionary spending. The manuscript highlights role empowerment, biohacking, in challenging reactive medical models, especially within Europe’s welfare-based systems. Drawing on research from systems biology, economics, lifestyle medicine, it calls a cultural shift toward healthspan-oriented care. also addresses barriers such as fragmented low literacy, proposes future persona models tools innovation.

Language: Английский

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