Graph-based 3D spatial gene neighborhood networks of single cells in gels and tissues
Zhou Fang,
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Kelsey Krusen,
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Henry D. Priest
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et al.
BME Frontiers,
Journal Year:
2025,
Volume and Issue:
6
Published: Jan. 1, 2025
Objective:
We
developed
3-dimensional
spatially
resolved
gene
neighborhood
network
embedding
(3D-spaGNN-E)
to
find
subcellular
proximity
relationships
and
identify
key
motifs
in
cell–cell
communication
(CCC).
Impact
Statement:
The
pipeline
combines
3D
imaging-based
spatial
transcriptomics
graph-based
deep
learning
motifs.
Introduction:
Advancements
imaging
experimental
technology
allow
the
study
of
capture
better
context
than
approximating
samples
as
2D.
However,
third
dimension
increases
data
complexity
requires
new
analyses.
Methods:
3D-spaGNN-E
detects
single
transcripts
cell
culture
identifies
relationships.
Then,
a
graph
autoencoder
projects
into
latent
space.
then
applied
explainability
analysis
CCC
Results:
first
mesenchymal
stem
cells
(MSCs)
cultured
hydrogel.
After
clustering
based
on
RNA
count,
we
identified
belonging
same
cluster
homotypic
those
different
clusters
heterotypic.
changes
local
near
border
between
heterotypic
cells.
When
applying
MSC–peripheral
blood
mononuclear
(PBMC)
coculture
system,
CD4
+
CD8
T
Local
can
distinguish
strong
weak
suppression
immune
Lastly,
compared
astrocyte–neuron
mouse
hypothalamus
cortex
by
analyzing
multiplexed-error-robust
fluorescence
situ
hybridization
(MERFISH)
regional
differences.
Conclusion:
distinguished
distinct
CCCs
tissue
examining
Language: Английский
Transformative Advances in Modeling Brain Aging and Longevity: Success, Challenges and Future Directions
Ageing Research Reviews,
Journal Year:
2025,
Volume and Issue:
unknown, P. 102753 - 102753
Published: April 1, 2025
Language: Английский
Emerging Trends in Microfluidic Biomaterials: From Functional Design to Applications
Lin Jiaqi,
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Lijuan Cui,
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Xiaokun Shi
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et al.
Journal of Functional Biomaterials,
Journal Year:
2025,
Volume and Issue:
16(5), P. 166 - 166
Published: May 8, 2025
The
rapid
development
of
microfluidics
has
driven
innovations
in
material
engineering,
particularly
through
its
ability
to
precisely
manipulate
fluids
and
cells
at
microscopic
scales.
Microfluidic
biomaterials,
a
cutting-edge
interdisciplinary
field
integrating
microfluidic
technology
with
biomaterials
science,
are
revolutionizing
biomedical
research.
This
review
focuses
on
the
functional
design
fabrication
organ-on-a-chip
(OoAC)
platforms
via
3D
bioprinting,
explores
applications
drug
delivery,
cell
culture,
tissue
evaluates
potential
systems
advancing
personalized
healthcare.
We
systematically
analyze
evolution
materials—from
silicon
glass
polymers
paper—and
highlight
advantages
bioprinting
over
traditional
methods.
Currently,
despite
significant
advances
medicine,
challenges
scalability,
stability,
clinical
translation
remain.
future
will
depend
combining
dynamic
design,
developing
hybrid
strategies
that
combine
molds
bio-printed
structures,
using
artificial
intelligence
monitor
delivery
or
response
real
time.
believe
collaborations
between
materials
micromachining,
medicine
accelerate
into
therapies
high-throughput
screening
tools.
Language: Английский
Actionable Health Investments for Longevity: Empowering Consumers to Shape the Healthcare Ecosystem
Roxana Mittler-Matica,
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Michael Friebe,
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Benjamin Niestroj
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et al.
Published: May 13, 2025
This
commentary
explores
the
rise
of
proactive
health
management
and
its
implications
for
consumer
behavior,
healthcare
systems,
long-term
outcomes.
It
argues
that
personalized,
preventive
care—via
digital
tools,
diagnostics,
supplementation—can
be
cost-effective
when
compared
to
routine
discretionary
spending.
The
manuscript
highlights
role
empowerment,
biohacking,
in
challenging
reactive
medical
models,
especially
within
Europe’s
welfare-based
systems.
Drawing
on
research
from
systems
biology,
economics,
lifestyle
medicine,
it
calls
a
cultural
shift
toward
healthspan-oriented
care.
also
addresses
barriers
such
as
fragmented
low
literacy,
proposes
future
persona
models
tools
innovation.
Language: Английский