The intestinal microbial community and function of Riptortus pedestris at different developmental stages and its effects on development

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 22, 2025

Introduction Riptortus pedestris is a destructive pest that threatens multiple leguminous crops in China. The intestinal microbiota plays crucial role the growth and reproduction of host insects. However, composition function gut at different developmental stages remain unclear. Methods Here, metagenomic sequencing was performed to clarify microbial diversity 2nd-, 3rd-, 4th-, 5th- instar nymphs (2 N–5 N) female adults (FAs) R. effects vital bacteria on development detected. have stage specificity, indicating their . Results Enterococcus Caballerronia were predominant present during 2 N–FAs. In addition, abundances 3 N 4 guts significantly greater than those others guts. Furthermore, 5 harbored abundant Burkholderia - Paraburkholderia Caballeronia metabolic pathways enriched from FAs. Carbohydrate metabolism, including glycoside hydrolases (GHs) glycosyl transferases (GTs), occurs throughout entire stage. Many antibiotic resistance genes (ARGs) detected Pseudomonadota Bacillota presented broad spectrum resistance. Excitingly, eliminated by treatment unable molt normally, lifespan shortened nymphs, had significant effect nymph development. Conclusion summary, our results, for first time, systematically illustrate abundance across demonstrate genera are This study provides basis stinkbug management strategies focus pivotal microbiota.

Language: Английский

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Metabolic synergy and complementarity in the Ips typographus holobiont

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Microbial symbiosis plays a central role in shaping ecological and evolutionary processes, driving the adaptation of host organisms to challenging environments. However, mechanisms underlying functional integration metabolic cooperation within holobionts remain poorly understood. Current research often emphasizes taxonomic composition microbiomes, but interactions that sustain these associations are less explored, especially nutrient-poor ecosystems. This study addresses this knowledge gap by investigating interdependencies gut microbiome European spruce bark beetle (Ips typographus, ESBB). Using meta-transcriptomic analyses, we reveal critical contributions bacterial fungal symbionts facilitating survival. Our findings show microbial partners compensate for deficiencies, with cross-kingdom enabling biosynthesis essential nutrients such as amino acids vitamins. Furthermore, division labor among taxa is evident, bacteria primarily degrading plant polymers xylan pectin, fungi specializing glucan degradation. Functional redundancy key pathways suggests an adaptive mechanism ensure nutrient availability under fluctuating community composition. In addition, identify previously unappreciated pathway nitrogen acquisition via oxidation inorganic nitrogen. highlight importance their success symbiotic associations. results provide framework exploring cycling resource use.

Language: Английский

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Ontogenetic Analysis of Chelonus formosanus and Diversity of Its Internal Microbiota

Insects, Journal Year: 2025, Volume and Issue: 16(2), P. 180 - 180

Published: Feb. 8, 2025

Chelonus formosanus is a parasitic wasp capable of parasitizing various Noctuidae pests, including the highly invasive Spodoptera frugiperda, and it demonstrates strong pest control potential. Both egg larval stages primarily occur within host organism, total developmental time from to adult approximately 19.62 days. To investigate microbial communities at different stages, we performed 16S rDNA sequencing (V1–V9 region) using PacBio identified 404 bacterial species belonging 61 classes, 116 orders, 182 families, 308 genera across larval, pupal, female, male stages. Bacterial diversity richness varied with Enterobacter Enterococcus dominating in larvae pupae Pseudomonas emerging as dominant female adults. In contrast, adults predominantly resided Ralstonia Achromobacter. The predicted functions bacteria C. are marked by high abundance metabolic pathways. This study provides comprehensive understanding morphology contributes practical pests. Additionally, our findings preliminarily characterized community laying groundwork for its functional study.

Language: Английский

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From soil to the intestinal tract: The key role of beneficial elements and probiotics in promoting health and longevity

Journal of Environmental Management, Journal Year: 2025, Volume and Issue: 384, P. 125611 - 125611

Published: May 1, 2025

Language: Английский

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Influence of Host Species, Location, and Aphid Prey on Microbial Diversity and Community Dynamics of Aphidophagous Ladybird Beetles in Guangxi, China

Ecology and Evolution, Journal Year: 2025, Volume and Issue: 15(2)

Published: Feb. 1, 2025

ABSTRACT Host species, locations, and diet can significantly impact microbial diversity community in insects. Several ladybird beetles are known as key predators potential biological control agents for aphids. However, there is limited understanding of how host aphid prey influence the aphidophagous natural environments. In this study, we collected 74 samples ladybirds their from various locations Guangxi, China, sequenced 16S amplicons to investigate differences microbiomes. The dominant genera samples, Bacteroides Alistipes , were rarely reported predominant other populations, indicating a unique genus‐level pattern Guangxi. Alpha indices Bray–Curtis distances varied among species. Abundance analysis revealed that relative abundance bacteria differed different species locations. Although primary facultative symbionts populations they had minimal direct on ladybirds, being sporadically detected corresponding predator samples. Our findings provide insights into communities aphids sympatric distinct field environments, highlighting plasticity across well low retention rate specific predators.

Language: Английский

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Advancements in the Impact of Insect Gut Microbiota on Host Feeding Behaviors

Genes, Journal Year: 2024, Volume and Issue: 15(10), P. 1320 - 1320

Published: Oct. 14, 2024

With the application and development of high-throughput sequencing technology, structure function insect gut microbiota have been analysed, which lays a foundation for further exploring intricate relationships between host feeding behaviour. The microbial community in gut, as an important ecological factor, affects host's food selection nutritional metabolic processes through various mechanisms, play key role population dynamics ecosystems. implications these interactions are profound, affecting agricultural practices, biodiversity, broader environment, such pollination pest control. In-depth exploration molecular mechanism interaction hosts contributes to grasp biology evolution offers novel avenues manipulating behaviour practical applications agriculture environmental management. This paper focuses on possible mechanisms regulating It inspires research insects

Language: Английский

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Role of Gut Bacteria in Enhancing Host Adaptation of Tuta absoluta to Different Host Plants

Insects, Journal Year: 2024, Volume and Issue: 15(10), P. 795 - 795

Published: Oct. 13, 2024

The insect gut bacteria play important roles in development and growth, such as immune defense, nutrient metabolism, regulating adaptations for plants, etc.

Language: Английский

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Limited Variation in Bacterial Communities of Scaphoideus titanus (Hemiptera: Cicadellidae) Across European Populations and Different Life Stages

Insects, Journal Year: 2024, Volume and Issue: 15(11), P. 830 - 830

Published: Oct. 23, 2024

The Nearctic leafhopper

Language: Английский

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