Isolation and Characterization of Lytic Phages Infecting Clinical Klebsiella pneumoniae from Tunisia DOI Creative Commons

Donia Mourali,

Rahma Kazdaghli,

Marwa Gara-Ali

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(12), P. 1154 - 1154

Published: Dec. 2, 2024

Background: Klebsiella pneumoniae is an opportunistic pathogen that causes a wide range of infections worldwide. The emergence and spread multidrug-resistant clones requires the implementation novel therapeutics, phages are promising approach. Results: In this study, two phages, KpTDp1 KpTDp2, were isolated from wastewater samples in Tunisia. These had narrow host specifically targeted hypervirulent K2 K28 capsular types K. pneumoniae. Both have double-stranded linear DNA genomes 49,311 49,084 bp, respectively. Comparative genomic phylogenetic analyses placed phage KpTDp2 genus Webervirus, while showed some homology with members Jedunavirus, although its placement new undescribed may be reconsidered. replication efficiency lytic ability these combined their high stability at temperatures up to 70 °C pH values ranging 3.5 8.2, highlight potential as good candidates for control Methods: Phage isolation, titration multiplicity infection performed. was tested different temperatures. Genomic characterization done by genome sequencing, annotation analysis. Conclusions: lyse one most virulent serotypes pneumoniae, well activities temperature variations, make antibacterial control.

Language: Английский

Klebsiella pneumoniae Phage M198 and Its Therapeutic Potential DOI Creative Commons

Lika Leshkasheli,

Ia Kusradze, D. Bolkvadze

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(1), P. 115 - 115

Published: Jan. 15, 2025

The rapid worldwide spread of antibiotic resistance is quickly becoming an increasingly concerning problem for human healthcare. Non-antibiotic antibacterial agents are in high demand many Gram-negative bacterial pathogens, including Klebsiella pneumoniae. Klebsiella-targeting phages among the most promising alternative therapy options. They have already been successfully applied a number cases, and it expected that need anti-Klebsiella will only increase future. This prospect highlights well-characterized therapeutic phages. In this work, we describe K. pneumoniae phage, which also infects strains oxytoca. Here, characterize phage M198 terms its biological genetic properties. Since some administered combination with antibiotics, here, screen possible synergistic effects combining six different antibiotics. We found has good lytic activity against clinical isolates; does not any indications temperate lifestyle, potential when combined therapeutically relevant

Language: Английский

Citations

0
Characterization of Novel Phages KPAФ1, KP149Ф1, and KP149Ф2 for lytic efficiency against Clinical MDR Klebsiella pneumoniae Infections DOI
Satya Dandekar,

Sinta Thanikkal,

Arti Londhe

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107440 - 107440

Published: Feb. 1, 2025

Language: Английский

Citations

0
Analysis of a novel phage as a promising biological agent targeting multidrug resistant Klebsiella pneumoniae DOI Creative Commons
Mahmoud A. AbdelRazek, Shaimaa I. Nazeih,

Nehal Yousef

et al.

AMB Express, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 5, 2025

The rise of deaths by resistant bacteria is a global threat to public health systems. Klebsiella pneumoniae virulent pathogen that causes serious nosocomial infections. major obstacle bacterial treatment antibiotic resistance, which necessitates the introducing alternative therapies. Phage therapy has been regarded as promising avenue fight multidrug-resistant (MDR) pathogens. In current study, novel phage vB_KpnP_KP17 was isolated from sewage, and its lytic potential investigated against K. pneumoniae. vB_KpnP_kP17 17.5% tested isolates. One step growth curve indicated with short latent period (20 min) large burst size (331 PFU/cell). Additionally, maintained activity planktonic cells over wide range pH, temperature UV irradiation intervals. antibiofilm agent revealed biofilm inhibition assay. at multiplicity infection (MOI) 10 inhibited more than 50% attached biofilms genome characterized found be linear dsDNA 39,936 bp in length GC content 52.85%. absence toxicity, virulence resistance genes further confirms safety for clinical applications. These characteristics make therapeutic value manage MDR formulation cocktail other phages or antibiotics could applied limit biofilm-producing

Language: Английский

Citations

0
Leveraging collateral sensitivity to counteract the evolution of bacteriophage resistance in bacteria DOI Creative Commons

YongQi Mu,

Yuqin Song, Xiaofei Tian

et al.

mLife, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Abstract The escalating antibiotic resistance crisis poses a major global health threat. Bacteriophage therapy offers promising alternative for combating multidrug‐resistant infections. However, bacterial to phages remains significant hurdle. Innovative strategies are needed overcome this challenge. In study, we developed phage cocktail based on our library, consisting of three that suppressed carbapenem‐resistant hypervirulent Klebsiella pneumoniae (CR‐hvKp). This capitalized dual instances collateral sensitivity, thereby constraining the evolution resistance. first‐layered sensitivity arose from overlapping coverage between capsular polysaccharide (CPS) and lipopolysaccharide (LPS), rendering bacteria resistant CPS‐binding but more susceptible LPS‐binding phages. second‐layered resulted an O serotype switch (from O1 O2), causing antigen‐binding increasing susceptibility target O2 antigen. dual‐layered effectively mitigated infection caused by CR‐hvKp in mice. Our research highlights importance mechanism counteracting sophisticated strategy configuring cocktails eliminate

Language: Английский

Citations

0
The Susceptibility of Klebsiella Pneumoniae Strains Isolated from COVID-19 Patients to Commercially Available Bacteriophage Medications DOI Creative Commons
Б. И. Асланов, A. Е. Goncharov, Sergey Konev

et al.

Antibiot Khimioter = Antibiotics and Chemotherapy, Journal Year: 2025, Volume and Issue: 69(11-12), P. 59 - 66

Published: April 19, 2025

Background . Superinfection caused by Klebsiella pneumoniae occupies a leading position in the structure of bacterial complications COVID-19 patients. The intensive circulation specialised hospitals has contributed to consolidation most clinically and epidemiologically important strains this pathogen, particular, representatives hypervirulent carbapenem-resistant clonal lines, which have not lost their relevance even post-pandemic period. use bacteriophages as therapeutic anti-epidemic agents seems justified given widespread multidrugresistant K. pneumoniae. Aim study To evaluate susceptibility associated with nosocomial infections patients polyvalent bacteriophage medications. Materials methods included 96 non-repeating isolated from clinical material admitted major hospital St. Petersburg severe moderate forms May 2020 January 2021. was assessed using spot test analysis. Commercially available preparations used for testing following: purified pyobacteriophage, sextaphage, pyobacteriophage. In order identify probable mechanisms resistance pneumoniae, nucleotide sequences genomes 6 pathogen belonging dominant genetic lines ST3, ST39, ST307, ST395, ST874 were studied. Results. Negative results tests observed 32.29% (95% CI=23.8–42.2) cases; general, proportion eligible treatment phage therapy 49% CI=39.2–58.8). Loci class 1 subtypes IV-A3 I-E, potentially CRISPR-Cas, identified genome studied strains, well number prophage bacteriophages. Conclusion demonstrated low activity medications against causing COVID-19. Increasing diversity active relevant clones can expand possibilities infections. rational containing these is possible within paradigm personalised therapy.

Language: Английский

Citations

0
Isolation and Characterization of Virulent Bacteriophages and Controlling Salmonella Enteritidis Biofilms on Chicken Meat DOI
Xiaowen Sun,

Fan Xue,

Cong Cong

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107619 - 107619

Published: April 1, 2025

Language: Английский

Citations

0
Isolation and Characterization of Lytic Phages Infecting Clinical Klebsiella pneumoniae from Tunisia DOI Creative Commons

Donia Mourali,

Rahma Kazdaghli,

Marwa Gara-Ali

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(12), P. 1154 - 1154

Published: Dec. 2, 2024

Background: Klebsiella pneumoniae is an opportunistic pathogen that causes a wide range of infections worldwide. The emergence and spread multidrug-resistant clones requires the implementation novel therapeutics, phages are promising approach. Results: In this study, two phages, KpTDp1 KpTDp2, were isolated from wastewater samples in Tunisia. These had narrow host specifically targeted hypervirulent K2 K28 capsular types K. pneumoniae. Both have double-stranded linear DNA genomes 49,311 49,084 bp, respectively. Comparative genomic phylogenetic analyses placed phage KpTDp2 genus Webervirus, while showed some homology with members Jedunavirus, although its placement new undescribed may be reconsidered. replication efficiency lytic ability these combined their high stability at temperatures up to 70 °C pH values ranging 3.5 8.2, highlight potential as good candidates for control Methods: Phage isolation, titration multiplicity infection performed. was tested different temperatures. Genomic characterization done by genome sequencing, annotation analysis. Conclusions: lyse one most virulent serotypes pneumoniae, well activities temperature variations, make antibacterial control.

Language: Английский

Citations

0