A Potential Link Between Oral Microbiota and Female Reproductive Health DOI Creative Commons
Justyna Marcickiewicz, Małgorzata Jamka, Jarosław Walkowiak

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 619 - 619

Published: March 7, 2025

Oral cavity dysbiosis is associated with numerous inflammatory diseases, including diabetes, bowel and periodontal disease. Changes in the oral microenvironment lead to bidirectional interactions between pathogens individual host systems, which may induce systemic inflammation. There increasing evidence linking condition of most common causes female infertility, such as polycystic ovary syndrome endometriosis, well gestational complications, e.g., low birth weight, preterm delivery, miscarriages. This review highlights composition microbiome relation infertility-related disorders, endometriosis syndrome, provides a comprehensive overview current state knowledge on relationship dysbiotic microbiome, pregnancy, its impact reproductive tract.

Language: Английский

Probiotic Therapy as an Adjuvant in the Treatment of Periodontal Disease: An Innovative Approach DOI Creative Commons
Liliana Sachelarie, Ioana Scrobotă, Ioana Romanul

et al.

Medicina, Journal Year: 2025, Volume and Issue: 61(1), P. 126 - 126

Published: Jan. 14, 2025

Background and Objectives: Periodontal inflammation, often linked to oral microbiota dysbiosis dominated by pathogenic bacteria, remains a significant challenge in periodontitis management. Traditional periodontal therapies primarily reduce the bacterial load but fail restore balance. Probiotics offer promising therapeutic adjunct with their ability enhance beneficial bacteria. This study investigates effects of probiotics on microbiota, inflammatory markers (IL-1β, TNF-α), clinical parameters (gingival index, bleeding pocket depth). Materials Methods: In this pilot study, 80 patients moderate-to-severe were assigned two groups. Group A received standard therapy (non-surgical (NSPT)) probiotic supplementation (Lactobacillus reuteri, 2 × 10⁹ CFU daily for 8 weeks), B treatment placebo. Microbiological changes assessed via quantitative PCR, while TNF-α) analyzed using ELISA. Clinical parameters, including gingival index (GI), (BI), depth (PPD), measured at baseline (T0), 4 weeks (T1), (T2) standardized methods. Results: Probiotic (Group A) significantly reduced bacteria increased levels compared placebo (p < 0.01). Inflammatory decreased 37% (IL-1β) 42% (TNF-α), improved, reductions indices (−1.5, −1.3) mm decrease Conclusions: Probiotics, as an therapy, effectively balance, improve outcomes periodontitis.

Language: Английский

Citations

2
Evaluation of Single and Multi-Strain Probiotics with Gentamicin Against E. coli O157:H7: Insights from In Vitro and In Vivo Studies DOI Creative Commons

Fatima H. Fneish,

Khaled H. Abd El Galil,

Souraya Domiati

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 460 - 460

Published: Feb. 19, 2025

The emergence of antibiotic-resistant food-borne pathogens, especially Escherichia coli O157:H7, highlights the urgent need for innovative treatment strategies, particularly in light rising resistances and ongoing controversy surrounding antibiotic use response to E. O157:H7 infections. To address this issue, we explored potential single- multi-strain probiotics, both independently combination with gentamicin, through a series vitro vivo experiments. In vitro, gentamicin alone produced mean inhibition zone 12.9 ± 2.27 mm against O157:H7. single-strain probiotics (P1) increased 16.5 2.24 (p < 0.05), while (P2) resulted largest 19 2.8 0.05). vivo, mice infected treated P2, (G), or their (G+P2), achieved 100% survival, no pathological symptoms, full weight recovery within seven days. Conversely, P1 G+P1 exhibited lower survival rates (71.4% 85%, respectively) slower recovery. Hematological parameters improved across all groups, but kidney function analysis showed significantly higher serum creatinine levels P1, G, G+P1, G+P2 groups compared P2 group (P1: 0.63 0.15 mg/dL; G: 0.34 0.09 G+P1: 0.53 0.19 G+P2: 0.5 0.23 mg/dL vs. P2: 0.24 0.2 mg/dL). Histological better intestinal tissue group, abnormal ileal structures severe cortical bleeding. These findings highlight promise conjunction antibiotics, as therapeutic strategy However, nephrotoxicity associated co-administration remains limitation, warranting further studies optimize approach.

Language: Английский

Citations

0
Evaluation of the anti-inflammatory, antioxidant and regenerative effects of microbiota-derived postbiotics in human periodontal ligament mesenchymal stromal cells DOI Creative Commons
Hazal Kibar Demirhan,

Emine Omer Oglou,

Zeynep Büşra Aksoy

et al.

Clinical Oral Investigations, Journal Year: 2025, Volume and Issue: 29(5)

Published: April 23, 2025

Abstract Objective This study investigates the regenerative and protective effects of postbiotics (cell-free supernatant) derived from Lactiplantibacillus plantarum EIR/IF-1 strain on human periodontal ligament mesenchymal stromal cells (hPDL-MSCs). Materials methods hPDL-MSCs were isolated tissues (PDL) wisdom teeth using enzymatic digestion subsequently characterized through immunophenotyping. The effect viability was assessed MTT assay flow cytometry, while their impact cell migration evaluated via scratch assay. Anti-inflammatory investigated lipopolysaccharide (LPS, Porphyromonas gingivalis )-stimulated Enzyme-Linked Immunosorbent Assay (ELISA). Additionally, antioxidant analyzed in hydrogen peroxide (H₂O₂)-induced by measuring reactive oxygen species (ROS) levels cytometry. expression collagen type I ( COL1A1 ) gene further quantitative reverse transcription PCR immunofluorescence staining. Results Treatment with (250 µg/mL) significantly increased capability hPDL-MSCs, enhancing production for PDL repair. 24 h resulted a 54.53 ± 2.01% reduction intracellular ROS compared to untreated H 2 O -induced hPDL-MSCs. Furthermore, decreased pro-inflammatory cytokines (IL-8, IL-6, IL-1β), anti-inflammatory cytokine IL-10 (2.67-fold) LPS-stimulated Conclusion Our findings indicate that exhibit biological activity throughout all stages healing process, beginning modulation inflammatory response LPS stimulation, followed promotion migration, proliferation, synthesis. Given unmet need safe adjuvant therapeutic approaches promote comprehensive regeneration diseases, this presents as promising candidate. Clinical relevance Postbiotics could be integrated into therapies novel bioactive material improve outcomes defects both controlling inflammation stimulating tissue repair processes.

Language: Английский

Citations

0
CAN PROBIOTICS REPLACE ANTIBIOTICS? NOT YET, SAYS the EVIDENCE DOI
Jan Łukasik, Hania Szajewska

Expert Review of Anti-infective Therapy, Journal Year: 2025, Volume and Issue: unknown

Published: May 10, 2025

Language: Английский

Citations

0
A Potential Link Between Oral Microbiota and Female Reproductive Health DOI Creative Commons
Justyna Marcickiewicz, Małgorzata Jamka, Jarosław Walkowiak

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 619 - 619

Published: March 7, 2025

Oral cavity dysbiosis is associated with numerous inflammatory diseases, including diabetes, bowel and periodontal disease. Changes in the oral microenvironment lead to bidirectional interactions between pathogens individual host systems, which may induce systemic inflammation. There increasing evidence linking condition of most common causes female infertility, such as polycystic ovary syndrome endometriosis, well gestational complications, e.g., low birth weight, preterm delivery, miscarriages. This review highlights composition microbiome relation infertility-related disorders, endometriosis syndrome, provides a comprehensive overview current state knowledge on relationship dysbiotic microbiome, pregnancy, its impact reproductive tract.

Language: Английский

Citations

0