Biofilm Dispersal in Bacillus velezensis FZB42 Is Regulated by the Second Messenger c-di-GMP DOI Creative Commons

Meiyu Zhang,

Shu Wu, Peng Chen

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 896 - 896

Published: April 13, 2025

Cyclic diguanosine monophosphate (c-di-GMP) is a second messenger that plays crucial role in regulating biofilm development, yet the Gram-positive bacteria remains elusive. Here, we demonstrated dispersed cells from biofilms of Bacillus velezensis FZB42 exhibit unique phenotype and gene expression compared to planktonic cells. Transcriptomic analysis revealed 1327 downregulated 1298 upregulated genes, among which c-di-GMP phosphodiesterase coding yuxH was remarkably upregulated. Deletion led elevated levels accompanied by reduced amounts “actively cells” pellicle capacity motility. spoIIIJ, spo0J, kinA resulted increased dispersal ability. Also, level when adding cues inhibition such as glucose calcium ions. Collectively, these present findings suggest negatively correlated with FZB42, sheds new light on regulation FZB42.

Language: Английский

Microbial diversity and interactions: Synergistic effects and potential applications of Pseudomonas and Bacillus consortia DOI
Lixue Wang, Xinyi Zhang, Jiahui Lü

et al.

Microbiological Research, Journal Year: 2025, Volume and Issue: 293, P. 128054 - 128054

Published: Jan. 7, 2025

Language: Английский

Citations

0
LXG Toxins of Bacillus Velezensis Mediate Contact-Dependent Inhibition in a T7SS-Dependent Manner to Enhance Rhizosphere Adaptability DOI Open Access
Xia Shu,

Xiting Sun,

Kesu Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2592 - 2592

Published: March 13, 2025

Rhizosphere bacteria always compete intensely for ecological niches, employing various strategies to inhibit the growth of microbial competitors. One such strategy, contact-dependent inhibition (CDI), involves direct delivery toxic proteins into competing neighboring by a secretion system, leading their growth. However, function CDI competition in natural environment remains unclear. In this study, we examined role type VII system (T7SS) substrate LXG domain-containing protein rhizobacterium Bacillus velezensis SQR9 and found that encodes toxins mediate against other strains biofilms. Transcriptional analysis revealed expression these lxg genes is induced root exudates positively correlates with T7SS gene cluster. We further confirmed survival mutants deficient was significantly decreased soil. These findings highlight critical its species rhizosphere, providing new insights importance environments.

Language: Английский

Citations

0
Role of iturin from Bacillus velezensis DMW1 in suppressing growth and pathogenicity of Plectosphaerella cucumerina in tomato by reshaping the rhizosphere microbial communities DOI
Qian Zhao, Qurban Ali,

Weiwei Yuan

et al.

Microbiological Research, Journal Year: 2025, Volume and Issue: unknown, P. 128150 - 128150

Published: March 1, 2025

Language: Английский

Citations

0
Biofilm Dispersal in Bacillus velezensis FZB42 Is Regulated by the Second Messenger c-di-GMP DOI Creative Commons

Meiyu Zhang,

Shu Wu, Peng Chen

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 896 - 896

Published: April 13, 2025

Cyclic diguanosine monophosphate (c-di-GMP) is a second messenger that plays crucial role in regulating biofilm development, yet the Gram-positive bacteria remains elusive. Here, we demonstrated dispersed cells from biofilms of Bacillus velezensis FZB42 exhibit unique phenotype and gene expression compared to planktonic cells. Transcriptomic analysis revealed 1327 downregulated 1298 upregulated genes, among which c-di-GMP phosphodiesterase coding yuxH was remarkably upregulated. Deletion led elevated levels accompanied by reduced amounts “actively cells” pellicle capacity motility. spoIIIJ, spo0J, kinA resulted increased dispersal ability. Also, level when adding cues inhibition such as glucose calcium ions. Collectively, these present findings suggest negatively correlated with FZB42, sheds new light on regulation FZB42.

Language: Английский

Citations

0