Adipose-derived mesenchymal stem cells (MSCs) are a superior cell source for bone tissue engineering

Bioactive Materials, Journal Year: 2023, Volume and Issue: 34, P. 51 - 63

Published: Dec. 14, 2023

Effective bone regeneration through tissue engineering requires a combination of osteogenic progenitors, osteoinductive biofactors and biocompatible scaffold materials. Mesenchymal stem cells (MSCs) represent the most promising seed for engineering. As multipotent that can self-renew differentiate into multiple lineages including fat, MSCs be isolated from numerous tissues exhibit varied differentiation potential. To identify an optimal progenitor cell source engineering, we analyzed proliferative activity potential four commonly-used mouse MSC sources, immortalized embryonic fibroblasts (iMEF), marrow stromal (imBMSC), calvarial mesenchymal progenitors (iCAL), adipose-derived (iMAD). We found iMAD exhibited highest adipogenic capabilities upon BMP9 stimulation

Language: Английский

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Piezocatalytically-induced controllable mineralization scaffold with bone-like microenvironment to achieve endogenous bone regeneration

Science Bulletin, Journal Year: 2024, Volume and Issue: 69(12), P. 1895 - 1908

Published: April 2, 2024

Language: Английский

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Remodeling Electrophysiological Microenvironment for Promoting Bone Defect Repair via Electret Hybrid Electrospun Fibrous Mat

Advanced Fiber Materials, Journal Year: 2024, Volume and Issue: unknown

Published: June 26, 2024

Language: Английский

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Focal adhesion and actin orientation regulated by cellular geometry determine stem cell differentiation via mechanotransduction

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 182, P. 81 - 92

Published: May 9, 2024

Language: Английский

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Cell-free biodegradable electroactive scaffold for urinary bladder tissue regeneration

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 2, 2025

Tissue engineering heavily relies on cell-seeded scaffolds to support the complex biological and mechanical requirements of a target organ. However, in addition safety efficacy, translation tissue technology will depend manufacturability, affordability, ease adoption. Therefore, there is need develop scalable biomaterial with sufficient bioactivity eliminate for exogenous cell seeding. Herein, we describe implementation an electroactive biodegradable elastomer urinary bladder engineering. To create electrically conductive mechanically robust scaffold regeneration, functionalization method wherein hydrophobic polymer poly(3,4-ethylenedioxythiophene) (PEDOT) polymerized situ within similarly citrate-based poly(octamethylene-citrate-co-octanol) (POCO) film. We demonstrate efficacy this augmentation primarily female athymic rats, comparing PEDOT-POCO mesenchymal stromal POCO scaffolds. recovers function anatomical structure comparably significantly better than non-cell-seeded This manuscript reports that confers electroactivity elastic scaffold, facilitating successful restoration physiological Cell seeding regenerative presents regulatory manufacturing hurdle. Here, authors report development free which can regeneration without seeding, demonstrating application repair.

Language: Английский

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Hydrogels with programmed spatiotemporal mechanical cues for stem cell-assisted bone regeneration

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 16, 2025

Hydrogels are extensively utilized in stem cell-based tissue regeneration, providing a supportive environment that facilitates cell survival, differentiation, and integration with surrounding tissues. However, designing hydrogels for regenerating hard tissues like bone presents significant challenges. Here, we introduce macroporous spatiotemporally programmed mechanical properties cell-driven regeneration. Using liquid-liquid phase separation interfacial supramolecular self-assembly of protein fibres, the structure provide ample space to prevent contact inhibition during proliferation. The rigid fibre-coated pore shell provides sustained cues guiding osteodifferentiation protecting against loads. Temporally, hydrogel exhibits tunable degradation rates can synchronize new deposition some extent. By integrating localized heterogeneity, structures, surface chemistry, regenerative degradability, demonstrate efficacy these cell-encapsulated rabbit porcine models. This marks substantial advancement tailoring cell-assisted

Language: Английский

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Tolerant and Rapid Endochondral Bone Regeneration Using Framework‐Enhanced 3D Biomineralized Matrix Hydrogels

Advanced Science, Journal Year: 2023, Volume and Issue: 11(9)

Published: Dec. 21, 2023

Abstract Tissue‐engineered bone has emerged as a promising alternative for defect repair due to the advantages of regenerative healing and physiological functional reconstruction. However, there is very limited breakthrough in achieving favorable regeneration harsh osteogenic microenvironment after injury, especially avascular hypoxic conditions. Inspired by developmental mode endochondral ossification, novel strategy proposed tolerant rapid using framework‐enhanced 3D biomineralized matrix hydrogels. First, it meticulously designed biomimetic hydrogels with both osteoinductive microenvironment, then integrated 3D‐printed polycaprolactone framework improve their mechanical strength structural fidelity. The inherent effectively activates marrow mesenchymal stem cells self‐regulation early‐stage chondrogenesis via TGFβ/Smad signaling pathway obstacle aerobic respiration. Meanwhile, strong created hybrid formulation native‐constitute inorganic salts, can synergistically regulate mineralization osteoclastic differentiation, thus accelerate late‐stage maturation. Furthermore, vivo ectopic osteogenesis situ skull successfully verified high efficiency maintenance mode, which offers treatment craniofacial repair.

Language: Английский

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Enabling Proregenerative Medical Devices via Citrate‐Based Biomaterials: Transitioning from Inert to Regenerative Biomaterials

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(6)

Published: Dec. 3, 2023

Abstract Regenerative medicine aims to restore tissue and organ function without the use of prosthetics permanent implants. However, achieving this goal has been elusive, field remains mostly an academic discipline with few products widely used in clinical practice. From a materials science perspective, barriers include lack proregenerative biomaterials, complex regulatory process demonstrate safety efficacy, user adoption challenges. Although particularly biodegradable polymers, can play major role regenerative medicine, their suboptimal mechanical degradation properties often limit use, they do not support inherent biological processes that facilitate regeneration. As 2020, nine synthetic polymers medical devices are cleared or approved for United States America. Despite limitations design, production, marketing these devices, small number dominated resorbable device market past 50 years. This perspective will review history applications highlight need requirements discuss path behind recent successful introduction citrate‐based biomaterials manufacturing innovative aimed at improving outcome musculoskeletal surgeries.

Language: Английский

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Exosome-functionalized heterogeneous nanofibrous scaffolds repair bone defects accompanied by muscle injury

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 485, P. 149681 - 149681

Published: Feb. 17, 2024

Language: Английский

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Engineered Niobium Carbide MXenzyme-Integrated Self-Adaptive Coatings Inhibiting Periprosthetic Osteolysis by Orchestrating Osteogenesis–Osteoclastogenesis Balance

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(23), P. 29805 - 29822

Published: June 3, 2024

Periprosthetic osteolysis induced by the ultrahigh-molecular-weight polyethylene (UHMWPE) wear particles is a major complication associated with sustained service of artificial joint prostheses and often necessitates revision surgery. Therefore, smart implant direct prevention repair abilities urgently developed to avoid painful Herein, we fabricate phosphatidylserine- polyethylenimine-engineered niobium carbide (Nb2C) MXenzyme-coated micro/nanostructured titanium (PPN@MNTi) that inhibits UHMWPE particle-induced periprosthetic osteolysis. The specific mechanism which PPN@MNTi operates involves bioresponsive release nanosheets from MNTi substrate within an microenvironment, initiated cleavage thioketal-dopamine molecule sensitive reactive oxygen species (ROS). Subsequently, functionalized Nb2C MXenzyme could target macrophages escape lysosomes, effectively scavenging intracellular ROS through its antioxidant nanozyme-mimicking activities. This further achieves suppression osteoclastogenesis inhibiting NF-κB/MAPK autophagy signaling pathways. Simultaneously, based on synergistic effect MXenzyme-integrated coatings topography, designed promotes osteogenic differentiation bone mesenchymal stem cells regulate homeostasis, achieving advanced osseointegration alleviable in vivo. study provides precise strategy osteolysis, offering paradigm for development orthopedic implants.

Language: Английский

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