Nature Communications,
Journal Year:
2019,
Volume and Issue:
10(1)
Published: Aug. 7, 2019
Abstract
Mammalian
embryos
change
shape
dramatically
upon
implantation.
The
cellular
and
molecular
mechanism
underlying
this
transition
are
largely
unknown.
Here,
we
show
that
is
directed
by
cross
talk
between
the
embryonic
epiblast
first
extra-embryonic
tissue,
trophectoderm.
Specifically,
via
visualisation
of
a
Cdx2-GFP
reporter
line
pharmacologically
mediated
loss
gain
function
experiments
provides
FGF
signal
results
in
differential
fate
acquisition
multipotent
trophectoderm
leading
to
formation
tissue
boundary
within
tissue.
becomes
essential
for
expansion
into
multi-layered
epithelium.
Folding
induces
spreading
second
primitive
endoderm.
Together,
these
events
remodel
pre-implantation
embryo
its
post-implantation
cylindrical
shape.
Our
findings
uncover
how
communication
tissues
positional
cues
drive
changes
mammalian
development
during
Cell Reports,
Journal Year:
2024,
Volume and Issue:
43(3), P. 113926 - 113926
Published: March 1, 2024
The
Hippo
signaling
pathway
is
a
central
growth
control
mechanism
in
multicellular
organisms.
By
integrating
diverse
mechanical,
biochemical,
and
stress
cues,
the
orchestrates
proliferation,
survival,
differentiation,
mechanics
of
cells,
which
turn
regulate
organ
development,
homeostasis,
regeneration.
A
deep
understanding
regulation
function
therefore
holds
great
promise
for
developing
novel
therapeutics
regenerative
medicine.
Here,
we
provide
updates
on
molecular
organization
mammalian
network,
review
regulatory
signals
functional
outputs
pathway,
discuss
roles
development
Cell Research,
Journal Year:
2023,
Volume and Issue:
33(9), P. 661 - 678
Published: July 17, 2023
Studies
of
cultured
embryos
have
provided
insights
into
human
peri-implantation
development.
However,
detailed
knowledge
lineage
development
as
well
underlying
mechanisms
remains
obscure.
Using
3D-cultured
embryos,
herein
we
report
a
complete
cell
atlas
the
early
post-implantation
lineages
and
decipher
cellular
composition
gene
signatures
epiblast
hypoblast
derivatives.
In
addition,
develop
an
embryo-like
assembloid
(E-assembloid)
by
assembling
naive
hESCs
extraembryonic
cells.
E-assembloids,
reveal
that
WNT,
BMP
Nodal
signaling
pathways
synergistically,
but
functionally
differently,
orchestrate
Specially,
dissect
mesoderm
endoderm
specifications.
Finally,
improved
E-assembloid
is
developed
to
recapitulate
tissue
architectures
in
pre-gastrulation
embryo.
Our
findings
provide
development,
offers
useful
model
disentangle
behaviors
interactions
drive
embryogenesis.
Nature Reviews Genetics,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 12, 2024
The
DNA
methylation
field
has
matured
from
a
phase
of
discovery
and
genomic
characterization
to
one
seeking
deeper
functional
understanding
how
this
modification
contributes
development,
ageing
disease.
In
particular,
the
past
decade
seen
many
exciting
mechanistic
discoveries
that
have
substantially
expanded
our
appreciation
for
generic,
evolutionarily
ancient
can
be
incorporated
into
robust
epigenetic
codes.
Here,
we
summarize
current
distinct
landscapes
emerge
over
mammalian
lifespan
discuss
they
interact
with
other
regulatory
layers
support
diverse
functions.
We
then
review
rising
interest
in
alternative
patterns
found
during
senescence
somatic
transition
cancer.
Alongside
advancements
single-cell
long-read
sequencing
technologies,
collective
insights
made
across
these
fields
offer
new
opportunities
connect
biochemical
genetic
features
cell
physiology,
developmental
potential
phenotype.
Review,
Smith
et
al.
describe
development
within
key
disease
states,
as
well
different
methyltransferases
interface
histone
modifications
proteins
create
maintain
them.
Nature Communications,
Journal Year:
2020,
Volume and Issue:
11(1)
Published: Aug. 10, 2020
Abstract
Aneuploidy,
the
presence
of
an
abnormal
number
chromosomes,
is
a
major
cause
early
pregnancy
loss
in
humans.
Yet,
developmental
consequences
specific
aneuploidies
remain
unexplored.
Here,
we
determine
extent
post-implantation
development
human
embryos
bearing
common
using
recently
established
culture
platform.
We
show
that
while
trisomy
15
and
21
develop
similarly
to
euploid
embryos,
monosomy
exhibit
high
rates
arrest,
16
display
hypo-proliferation
trophoblast,
tissue
forms
placenta.
Using
trophoblast
stem
cells,
this
phenotype
can
be
mechanistically
ascribed
increased
levels
cell
adhesion
protein
E-CADHERIN,
which
lead
premature
differentiation
cycle
arrest.
identify
three
cases
mosaicism
diagnosed
as
full
aneuploid
by
pre-implantation
genetic
testing.
Our
results
present
first
detailed
analysis
embryos.
Advanced Materials,
Journal Year:
2021,
Volume and Issue:
33(43)
Published: Sept. 9, 2021
Abstract
Organoids
are
lumen‐containing
multicellular
structures
that
recapitulate
key
features
of
the
organs,
and
increasingly
used
in
models
disease,
drug
testing,
regenerative
medicine.
Recent
work
has
3D
culture
to
form
organoids
from
human
induced
pluripotent
stem
cells
(hiPSCs)
reconstituted
basement
membrane
(rBM)
matrices.
However,
rBM
matrices
offer
little
control
over
microenvironment.
More
generally,
role
matrix
viscoelasticity
directing
lumen
formation
remains
unknown.
Here,
viscoelastic
alginate
hydrogels
with
independently
tunable
stress
relaxation
(viscoelasticity),
stiffness,
arginine–glycine–aspartate
(RGD)
ligand
density
study
hiPSC
morphogenesis
culture.
A
phase
diagram
shows
how
these
properties
is
reported.
Higher
RGD
fast
promote
viability,
proliferation,
apicobasal
polarization,
formation,
while
slow
at
low
densities
triggers
apoptosis.
Notably,
hiPSCs
maintain
pluripotency
for
much
longer
times
than
reported
Lumen
regulated
by
actomyosin
contractility
accompanied
translocation
Yes‐associated
protein
(YAP)
nucleus
cytoplasm.
The
results
reveal
as
a
potent
factor
regulating
cell
provide
new
insights
into
engineered
biomaterials
may
be
leveraged
build
organoids.
