Treacle’s ability to form liquid-like phase condensates is essential for nucleolar fibrillar center assembly, efficient rRNA transcription and processing, and rRNA gene repair DOI Creative Commons

Artem K. Velichko,

Nadezhda V. Petrova,

Dmitry A Deriglazov

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: April 14, 2025

We investigated the role of nucleolar protein Treacle in organizing and regulating nucleolus human cells. Our results support Treacle’s ability to form liquid-like phase condensates through electrostatic interactions among molecules. The formation these biomolecular is crucial for segregating fibrillar centers from dense component ensuring high levels ribosomal RNA (rRNA) gene transcription accurate rRNA processing. Both central C-terminal domains are required condensates. initiation separation attributed domain. domain characterized by repeated stretches alternatively charged amino acid residues vital condensate stability. Overexpression mutant forms that cannot compromises assembly centers, suppressing disrupting These also fail recruit DNA topoisomerase II binding 1 (TOPBP1), damage response nucleolus.

Language: Английский

Nucleic Acids and Electrical Signals DOI
Colin McCaig

Reviews of physiology, biochemistry and pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 147 - 193

Published: Jan. 1, 2025

Language: Английский

Citations

0
Liquid condensates: a new barrier to loop extrusion? DOI Creative Commons

Arseniy V. Selivanovskiy,

Maria N. Molodova,

Ekaterina E. Khrameeva

et al.

Cellular and Molecular Life Sciences, Journal Year: 2025, Volume and Issue: 82(1)

Published: Feb. 20, 2025

Liquid–liquid phase separation (LLPS), driven by dynamic, low-affinity multivalent interactions of proteins and RNA, results in the formation macromolecular condensates on chromatin. These structures are likely to provide high local concentrations effector factors responsible for various processes including transcriptional regulation DNA repair. In particular, enhancers, super-enhancers, promoters serve as platforms condensate assembly. current paradigm, enhancer-promoter (EP) interaction could be interpreted a result enhancer- promoter-based contact/fusion. There is increasing evidence that spatial juxtaposition enhancers provided loop extrusion (LE) SMC complexes. Here, we propose may act barriers LE, thereby contributing nuclear contacts between regulatory genomic elements.

Language: Английский

Citations

0
The DEAD-box helicase eIF4A1/2 acts as RNA chaperone during mitotic exit enabling chromatin decondensation DOI Creative Commons
Ramona Jühlen,

Sabine C. Wiesmann,

Anja Scheufen

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 11, 2025

Abstract During mitosis, chromosomes condense and decondense to segregate faithfully undamaged. The exact molecular mechanisms are not well understood. We identify the DEAD-box helicase eIF4A1/2 as a critical factor in this process. In cell-free condensation assay is crucial for process, relying on its RNA-binding ability but ATPase activity. Reducing levels cells consistently slows down chromatin decondensation during nuclear reformation. Conversely, increasing concentration mitotic accelerates their decondensation. absence of affects perichromatin layer, which surrounds mitosis consists RNA mainly nucleolar proteins. vitro, acts an chaperone, dissociating biomolecular condensates chaperone activity required regulate composition fluidity dynamic reorganization exit mitosis.

Language: Английский

Citations

0
Treacle’s ability to form liquid-like phase condensates is essential for nucleolar fibrillar center assembly, efficient rRNA transcription and processing, and rRNA gene repair DOI Open Access

Artem K. Velichko,

Nadezhda V. Petrova,

Dmitry A Deriglazov

et al.

Published: March 31, 2025

We investigated the role of nucleolar protein Treacle in organizing and regulating nucleolus human cells. Our results support Treacle’s ability to form liquid-like phase condensates through electrostatic interactions among molecules. The formation these biomolecular is crucial for segregating fibrillar centers from dense component ensuring high levels rRNA gene transcription accurate processing. Both central C-terminal domains are required condensates. initiation separation attributed domain. domain characterized by repeated stretches alternatively charged amino-acid residues vital condensate stability. Overexpression mutant forms that cannot compromises assembly centers, suppressing disrupting These also fail recruit DNA topoisomerase II binding 1 (TOPBP1), damage response nucleolus.

Language: Английский

Citations

0
Treacle’s ability to form liquid-like phase condensates is essential for nucleolar fibrillar center assembly, efficient rRNA transcription and processing, and rRNA gene repair DOI Creative Commons

Artem K. Velichko,

Nadezhda V. Petrova,

Dmitry A Deriglazov

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: April 14, 2025

We investigated the role of nucleolar protein Treacle in organizing and regulating nucleolus human cells. Our results support Treacle’s ability to form liquid-like phase condensates through electrostatic interactions among molecules. The formation these biomolecular is crucial for segregating fibrillar centers from dense component ensuring high levels ribosomal RNA (rRNA) gene transcription accurate rRNA processing. Both central C-terminal domains are required condensates. initiation separation attributed domain. domain characterized by repeated stretches alternatively charged amino acid residues vital condensate stability. Overexpression mutant forms that cannot compromises assembly centers, suppressing disrupting These also fail recruit DNA topoisomerase II binding 1 (TOPBP1), damage response nucleolus.

Language: Английский

Citations

0