Exploration of RNA-binding proteins identified RPS27 as a potential regulator associated with Kaposi’s sarcoma development DOI Creative Commons

Jingzhan Zhang,

Peng Wang, Tingting Li

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 27, 2025

Kaposi's sarcoma (KS) is a locally aggressive, multicentric tumor. RNA-binding proteins (RBPs) are pivotal for post-transcriptional regulation in various tumors. However, the aberrantly expressed RBP genes and their regulatory patterns KS remain unclear. This study aimed to identify relevant assess potential functions molecular interactions of RPS27, dysregulated tissues, Matched lesions normal control tissues from ten patients were chosen study. Differentially (DEGs) first identified by RNA-sequencing, results validated through an independent public RNA-seq dataset (GSE147704). Among DEGs, RBPs selected further analysis, with RPS27 detailed investigation due its dysregulation tissues. RT-qPCR immunohistochemistry employed validate expression. Cellular experiments conducted explore functions. Additionally, improved RNA immunoprecipitation (iRIP)-seq was performed investigate binding KS. We 828 DEGs RNA-seq, 367 overlapping confirmed dataset. obtained 48 including 3 upregulated (PCBP3, L1TD1, PEG10) 45 downregulated Notably, included TECR, PUSL1, DQX1, MAT1A, RACK1, EEF1A2, EEF1B2, remaining all ribosomal protein genes, which exploration. that inhibition could promote cellular proliferation, migration, invasion, angiogenesis HUVECs, consistent downregulation iRIP-seq analyses showed RPS27's ability selectively bind 26 correlation. The majority RPS27-bound RPL8, RPL13, RPL13A, RPL18, RPL19, RPL23, RPLP1, RPL27A, RPL40, RPS2, RPS4X, RPS13, RPS18, RPS21, associated viral transcription gene Our explored targets indicating role development.

Language: Английский

Regulating MYC translation in cancer DOI
Zainab Hussain, Mara H. Sherman

Nature Cell Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

Language: Английский

Citations

0
Exploration of RNA-binding proteins identified RPS27 as a potential regulator associated with Kaposi’s sarcoma development DOI Creative Commons

Jingzhan Zhang,

Peng Wang, Tingting Li

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 27, 2025

Kaposi's sarcoma (KS) is a locally aggressive, multicentric tumor. RNA-binding proteins (RBPs) are pivotal for post-transcriptional regulation in various tumors. However, the aberrantly expressed RBP genes and their regulatory patterns KS remain unclear. This study aimed to identify relevant assess potential functions molecular interactions of RPS27, dysregulated tissues, Matched lesions normal control tissues from ten patients were chosen study. Differentially (DEGs) first identified by RNA-sequencing, results validated through an independent public RNA-seq dataset (GSE147704). Among DEGs, RBPs selected further analysis, with RPS27 detailed investigation due its dysregulation tissues. RT-qPCR immunohistochemistry employed validate expression. Cellular experiments conducted explore functions. Additionally, improved RNA immunoprecipitation (iRIP)-seq was performed investigate binding KS. We 828 DEGs RNA-seq, 367 overlapping confirmed dataset. obtained 48 including 3 upregulated (PCBP3, L1TD1, PEG10) 45 downregulated Notably, included TECR, PUSL1, DQX1, MAT1A, RACK1, EEF1A2, EEF1B2, remaining all ribosomal protein genes, which exploration. that inhibition could promote cellular proliferation, migration, invasion, angiogenesis HUVECs, consistent downregulation iRIP-seq analyses showed RPS27's ability selectively bind 26 correlation. The majority RPS27-bound RPL8, RPL13, RPL13A, RPL18, RPL19, RPL23, RPLP1, RPL27A, RPL40, RPS2, RPS4X, RPS13, RPS18, RPS21, associated viral transcription gene Our explored targets indicating role development.

Language: Английский

Citations

0