Neuronal activity modulates the incorporation of newly translated PSD-95 into a robust structure as revealed by STED and MINFLUX DOI Creative Commons
Clara‐Marie Gürth, Maria Augusta do Rego Barros Fernandes Lima, Victor Macarrón‐Palacios

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 20, 2023

Abstract The postsynaptic density component PSD-95 undergoes activity-dependent plasticity mechanisms that rely on protein synthesis and structural remodeling. How synaptic activity can influence these dynamics at the single synapse level remains unclear. Here we combine genome-editing, pulse-chase experiments, STED 3D MINFLUX nanoscopy hippocampal neuronal cultures to study integration of newly translated molecules sites their rearrangement within individual clusters near-molecular resolution. We show amount recruited synapses scales with size, modulates in a bidirectional manner, resulting less new following excitatory more inhibitory stimulation. Furthermore, has dispersed organization is largely robust long-lasting changes activity. Altogether, this work sheds light underlying level, adding previously inaccessible information.

Language: Английский

Coordinating the Edge Defects of Bismuth with Sulfur for Enhanced CO2 Electroreduction to Formate DOI

Lei Lv,

Ruihu Lu, Jiexin Zhu

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(25)

Published: April 20, 2023

Bismuth-based materials have been recognized as promising catalysts for the electrocatalytic CO2 reduction reaction (ECO2 RR). However, they show poor selectivity due to competing hydrogen evolution (HER). In this study, we developed an edge defect modulation strategy Bi by coordinating defects of bismuth (Bi) with sulfur, promote ECO2 RR and inhibit HER. The prepared demonstrate excellent product selectivity, a high HCOO- Faraday efficiency ≈95 % partial current ≈250 mA cm-2 under alkaline electrolytes. Density function theory calculations reveal that sulfur tends bind defects, reducing coordination-unsaturated sites (*H adsorption sites), regulating charge states neighboring improve *OCHO adsorption. This work deepens our understanding mechanism on bismuth-based catalysts, guiding design advanced catalysts.

Language: Английский

Citations

87
Hybrid Small-Molecule/Protein Fluorescent Probes DOI
Masafumi Minoshima, Shahi Imam Reja,

Ryu Hashimoto

et al.

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(10), P. 6198 - 6270

Published: May 8, 2024

Hybrid small-molecule/protein fluorescent probes are powerful tools for visualizing protein localization and function in living cells. These hybrid constructed by diverse site-specific chemical labeling approaches through reactions to exogenous peptide/small tags, enzymatic post-translational modifications, bioorthogonal genetically incorporated unnatural amino acids, ligand-directed reactions. The employed imaging trafficking, conformational changes, bioanalytes surrounding proteins. In addition, facilitate visualization of dynamics at the single-molecule level defined structure with super-resolution imaging. this review, we discuss development bioimaging applications based on hybrids.

Language: Английский

Citations

24
Photoactivation Inducing Multifunctional Coupling of Fluorophore for Efficient Tumor Therapy In Situ DOI
Weijie Zhang,

Yunxia Lv,

Fangjun Huo

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(23)

Published: Feb. 15, 2024

Abstract Photoactivatable molecules, with high‐precision spatialtemporal control, have largely promoted bioimaging and phototherapy applications of fluorescent dyes. Here, the first photoactivatable sensor ( BI ) is described that can be triggered by broad excitation light (405–660 nm), which further undergoes intersystem crossing H‐atom transfer processes to forming superoxide anion radicals (O 2 −• carbon radicals. Particularly, photoinduced gain carbon‐centered BI• allows for radical‐radical coupling afford combined crosslink product BI─BI ), would oxidized in presence O produce an extended conjugate system near infrared emission (820 nm). Besides, photochemically generated Cy─BI possesses ultra‐high photothermal conversion efficiency up 90.9%, optimized potential. What′s more, Western Blot assay reveals both photoproduct efficiently inhibit expression CHK1, irradiation induces apoptosis ultimately enhances phototherapeutic effects. Thus, combination cell cycle block inducing apoptosis, photodynamic therapy treatments significantly suppress solid tumor vivo antitumor efficacy explorations. This a novel finding developing as well applicability photoimaging tumor‐related areas.

Language: Английский

Citations

19
Biosynthesis and genetic encoding of activated nitriles for fast protein conjugation and tunable fluorogenic labeling DOI
Elwy H. Abdelkader, Haocheng Qianzhu, Gottfried Otting

et al.

Chem, Journal Year: 2025, Volume and Issue: unknown, P. 102385 - 102385

Published: Jan. 1, 2025

Language: Английский

Citations

2
Long-Wavelength Photoconvertible Dimeric BODIPYs for Super-Resolution Single-Molecule Localization Imaging in Near-Infrared Emission DOI
Qingbao Gong, Xinfu Zhang, Wanwan Li

et al.

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(48), P. 21992 - 21999

Published: Nov. 22, 2022

Sulfoxide-bridged dimeric BODIPYs were developed as a new class of long-wavelength photoconvertible fluorophores. Upon visible-light irradiation, sulfoxide moiety was released to generate the corresponding α,α-directly linked BODIPYs. The extrusion SO from sulfoxides mainly through an intramolecular fashion involving reactive triplet states. By this photoconversion, not only more than 100 nm red shifts absorption and emission maxima (up 648/714 nm) achieved but also stable products with bright fluorescence produced high efficiency. combination photoactivation red-shifted excitation/emission offered optimal contrast eliminated interference biological autofluorescence. More importantly, in situ these visible-light-induced reactions demonstrated ideal single-molecule properties near-infrared region. Therefore, photoconversion could be powerful method achieving super-resolution localization imaging living cell without using UV illumination cell-toxic additives.

Language: Английский

Citations

53
HaloTag‐Based Reporters for Fluorescence Imaging and Biosensing DOI Creative Commons
Alexander Cook, Franziska Walterspiel, Claire Deo

et al.

ChemBioChem, Journal Year: 2023, Volume and Issue: 24(12)

Published: Feb. 23, 2023

Abstract Visualizing the structure and dynamics of biomolecules is critical to understand biological function, requires methods fluorescently label targets interest in their cellular context. Self‐labelling proteins, which combine a genetically encoded tag with small‐molecule fluorophore, have attracted considerable attention for this purpose, as they can overcome limitations fluorescent proteins. Among them, HaloTag protein most broadly used, showing fast specific labelling small, easy functionalize cell‐permeant ligand. Synthetic chemistry engineering provided portfolio powerful imaging tools exploiting HaloTag, along general optimize adapt them applications. Here, we provide an overview reporters based on biosensing, highlighting strategies

Language: Английский

Citations

36
Photoactivatable Large Stokes Shift Fluorophores for Multicolor Nanoscopy DOI Creative Commons

Ilya Likhotkin,

Richard Lincoln, Mariano L. Bossi

et al.

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(3), P. 1530 - 1534

Published: Jan. 10, 2023

We designed caging-group-free photoactivatable live-cell permeant dyes with red fluorescence emission and ∼100 nm Stokes shifts based on a 1-vinyl-10-silaxanthone imine core structure. The proposed fluorophores undergo byproduct-free one- two-photon activation, are suitable for multicolor microscopy in fixed living cells, compatible super-resolution techniques such as STED (stimulated depletion) PALM (photoactivated localization microscopy). Use of labels strain-promoted tetrazine ligation self-labeling protein tags (HaloTag, SNAP-tag), duplexing an imaging channel another large shift dye have been demonstrated.

Language: Английский

Citations

33
Accelerated MINFLUX Nanoscopy, through Spontaneously Fast‐Blinking Fluorophores DOI Creative Commons
Michael Remmel, Lukas Scheiderer,

Alexey N. Butkevich

et al.

Small, Journal Year: 2023, Volume and Issue: 19(12)

Published: Jan. 15, 2023

The introduction of MINFLUX nanoscopy allows single molecules to be localized with one nanometer precision in as little millisecond. However, current applications have so far focused on increasing this by optimizing photon collection, rather than minimizing the localization time. Concurrently, commonly used fluorescent switches are specifically designed for stochastic methods (e.g., STORM), optimized a high yield and long on-times (tens milliseconds). Here, accelerated up 30-fold gain speed is presented. improvement attained designing spontaneously blinking markers remarkably fast on-times, down 1-3 ms, matching iterative process microscope. This design utilizes silicon rhodamine amide core, shifting spirocyclization equilibrium toward an uncharged closed form at physiological conditions imparting intact live cell permeability, modified fused (benzo)thiophene spirolactam fragment. best candidate microscopy (also suitable STORM imaging) selected through detailed characterization behavior fluorophores, bound different protein tags. Finally, optimization routines, customized times, renders significant commercial

Language: Английский

Citations

25
Targeted Photoconvertible BODIPYs Based on Directed Photooxidation-Induced Conversion for Applications in Photoconversion and Live Super-Resolution Imaging DOI
Lazare Saladin, Victor Breton, Valentine Le Berruyer

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(25), P. 17456 - 17473

Published: June 11, 2024

Photomodulable fluorescent probes are drawing increasing attention due to their applications in advanced bioimaging. Whereas photoconvertible can be advantageously used tracking, photoswitchable constitute key tools for single-molecule localization microscopy perform super-resolution imaging. Herein, we shed light on a red and far-red BODIPY, namely, BDP-576 BDP-650, which possess both properties of conversion switching. Our study demonstrates that these pyrrolyl-BODIPYs convert into typical green- red-emitting BODIPYs perfectly adapted microscopy. We also showed this undergo Directed Photooxidation Induced Conversion, photoconversion mechanism recently introduced, where the pyrrole moiety plays central role. These unique features were develop targeted toward different organelles or subcellular units (plasma membrane, mitochondria, nucleus, actin, Golgi apparatus, etc.) using chemical targeting moieties Halo tag. notably BDP-650 could track intracellular vesicles over more than 20 min two-color imagings with laser scanning confocal microscopy, demonstrating its robustness. The switching photoconverters studied at level then successfully live epithelial cells neurons. Both membrane- mitochondria- decipher membrane 3D architecture mitochondrial dynamics nanoscale. This builds bridge between photoswitching undergoing directed photooxidation shows versatility efficacy

Language: Английский

Citations

11
Biomimetic Trypsin-Responsive Structure-Bridged Mesoporous Organosilica Nanomedicine for Precise Treatment of Acute Pancreatitis DOI
Yanan Wang,

Deyao Qian,

Xinyuan Wang

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(29), P. 19283 - 19302

Published: July 11, 2024

Developing strategies to target injured pancreatic acinar cells (PACs) in conjunction with primary pathophysiology-specific pharmacological therapy presents a challenge the management of acute pancreatitis (AP). We designed and synthesized trypsin-cleavable organosilica precursor bridged by arginine-based amide bonds, leveraging trypsin's ability selectively identify guanidino groups on arginine via Asp189 at active S1 pocket cleave carboxy-terminal (C-terminal) bond catalytic triads. The precursors were incorporated into framework mesoporous silica nanoparticles (MSNs) for encapsulating membrane-permeable Ca

Language: Английский

Citations

10