Stabilization of Condensate Interfaces Using Dynamic Protein Insertion DOI Creative Commons

Yannick H. A. Leurs,

Sanne N. Giezen,

Yudong Li

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: May 24, 2025

Coacervates have been widely used to mimic membraneless organelles (MLOs). However, coacervates without a membrane or stabilizing surface do not feature the same level of stability as MLOs. This study shows that specifically engineered surface-active proteins can interact with interface polypeptide coacervates, conferring resistance coacervate dissolution and fusion. Modulating molecular characteristics these highlighted their dimerization aids in achieving effective stabilizers. Cryo-TEM imaging showed densely packed protein monolayer at coacervate-liquid interface, while single-molecule super-resolution microscopy captured dynamic nature this layer, rapidly (un)docking moving across within milliseconds. These findings suggest form stabilization driven by transient interactions condensate interface. unique only provides new approach developing stable dynamically exchanging synthetic systems but, model systems, also significantly contribute our understanding mechanisms underlying temporal MLOs nature.

Language: Английский

A programmable biomimetic cytoskeleton DOI
Henrike Niederholtmeyer

Nature Chemistry, Journal Year: 2025, Volume and Issue: 17(3), P. 311 - 313

Published: Feb. 24, 2025

Language: Английский

Citations

0
Stabilization of Condensate Interfaces Using Dynamic Protein Insertion DOI Creative Commons

Yannick H. A. Leurs,

Sanne N. Giezen,

Yudong Li

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: May 24, 2025

Coacervates have been widely used to mimic membraneless organelles (MLOs). However, coacervates without a membrane or stabilizing surface do not feature the same level of stability as MLOs. This study shows that specifically engineered surface-active proteins can interact with interface polypeptide coacervates, conferring resistance coacervate dissolution and fusion. Modulating molecular characteristics these highlighted their dimerization aids in achieving effective stabilizers. Cryo-TEM imaging showed densely packed protein monolayer at coacervate-liquid interface, while single-molecule super-resolution microscopy captured dynamic nature this layer, rapidly (un)docking moving across within milliseconds. These findings suggest form stabilization driven by transient interactions condensate interface. unique only provides new approach developing stable dynamically exchanging synthetic systems but, model systems, also significantly contribute our understanding mechanisms underlying temporal MLOs nature.

Language: Английский

Citations

0