Cell, Journal Year: 2022, Volume and Issue: 185(17), P. 3138 - 3152.e20
Published: Aug. 1, 2022
Language: Английский
Proceedings of the National Academy of Sciences, Journal Year: 2017, Volume and Issue: 114(40), P. 10701 - 10706
Published: Sept. 18, 2017
Significance Our study assesses the long-held hypothesis that evolution of new gene functions underlies diversification animal forms. To do this, we systematically compared patterning roles a single across seven butterfly species. Under null stasis, each knockout experiment should yield directly comparable phenotypes. We instead observed varied repertoire lineage-specific effects in different wing regions, demonstrating repeated modification key instructive signal was instrumental complex color patterns. These comparative data confirm heuristic potential CRISPR mutagenesis nontraditional model organisms and illustrate principle biodiversity can emerge from tinkering homologous genetic factors.
Language: Английский
Citations
151
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2016, Volume and Issue: unknown
Published: June 6, 2016
Abstract As the generation and use of genomic datasets is becoming increasingly common in all areas biology, need for resources to collate, analyse present data from independent (Tier 1) species-level genome projects into well supported clade-oriented 2) databases provide a mechanism these be propagated pan-taxonomic 3) more pressing. Lepbase Tier 2 resource Lepidoptera, supporting research community using approaches understand evolution, speciation, olfaction, behaviour pesticide resistance wide range target species. offers core set tools make widely accessible including an Ensembl browser, text sequence homology searches bulk downloads consistently presented formatted datasets. part taxonomic that we serve, are working directly with Lepidoptera researchers prioritise analyses add will most value current questions.
Language: Английский
Citations
149
Methods in Ecology and Evolution, Journal Year: 2017, Volume and Issue: 9(2), P. 390 - 398
Published: July 11, 2017
The use of image data to quantify, study and compare variation in the colors patterns organisms requires alignment images establish homology, followed by color-based segmentation images. Here we describe an R package for that has applications quantify color a wide range organisms. patternize is quantifies obtained from data. first defines homology between pattern positions across specimens either through manually placed homologous landmarks or automated registration. Pattern identification performed categorizing distribution using RGB threshold, k-means clustering watershed transformation.We demonstrate can be used quantification variety analyzing butterflies, guppies, spiders salamanders. Image compared sets specimens, visualized as heatmaps analyzed principal component analysis (PCA). potential fine scale phenotypes population comparisons, genetic association studies investigating basis
Language: Английский
Citations
121
Proceedings of the National Academy of Sciences, Journal Year: 2019, Volume and Issue: 116(48), P. 24174 - 24183
Published: Nov. 11, 2019
Color pattern mimicry in Heliconius butterflies is a classic case study of complex trait adaptation via selection on few large effect genes. Association studies have linked color variation to handful noncoding regions, yet the presumptive cis-regulatory elements (CREs) that control patterning remain unknown. Here we combine chromatin assays, DNA sequence associations, and genome editing functionally characterize 5 gene optix . We were surprised find architecture characterized by pleiotropy regulatory fragility, where deletion individual has broad effects both wing vein development. Remarkably, found orthologous associate with convergence distantly related comimics, suggesting parallel coevolution ancestral facilitated mimicry. Our results support model evolution changes ancient, multifunctional underlie adaptive radiation.
Language: Английский
Citations
120
PLoS Biology, Journal Year: 2019, Volume and Issue: 17(2), P. e2005902 - e2005902
Published: Feb. 7, 2019
The evolution of new species is made easier when traits under divergent ecological selection are also mating cues. Such cues now considered more common than previously thought, but we still know little about the genetic changes underlying their or generally basis for assortative behaviors. Both tight physical linkage and existence large-effect preference loci will strengthen associations between behavioral barriers, promoting mating. warning patterns Heliconius melpomene H. cydno disruptive due to increased predation nonmimetic hybrids used during mate recognition. We carried out a genome-wide quantitative trait locus (QTL) analysis behaviors these showed that male has simple basis. identify three QTLs together explain large proportion (approximately 60%) difference in behavior observed parental species. One just 1.2 (0-4.8) centiMorgans (cM) from major color pattern gene optix, and, individually, all have effect on phenotype. Genomic divergence high broadly heterogenous, admixture reduced at preference-optix not other QTLs. architecture reveal facilitate maintenance despite ongoing flow by coupling aspects reproductive isolation.
Language: Английский
Citations
103
Conservation Genetics, Journal Year: 2019, Volume and Issue: 20(1), P. 101 - 113
Published: Feb. 1, 2019
Species delimitation is one of the most contested areas in modern biology, with widespread disagreement about almost every aspect definition and implementation "species" label. While this debate intellectually stimulating, it also has real implications for conservation, where its impacts on taxonomic inflation or inertia can mean that specific populations receive adequate conservation measures are ignored. Recently, rise next generation sequencing phylogenomics revolutionised phylogenetic understanding many organismal groups but simultaneously highlighted porosity genomes terms admixture across previously delineated species barriers. The extraordinary power genomic data increasingly being used to delineate species, several publications domain have recently attracted significant attention criticism. Here we revisit question delimitation, from a context. We ask how whether large amounts provided by methods resolve longstanding discussion validity application allied concepts, some recent examples inform debate. argue conserving adaptive potential priority no single concept currently does adequately own. Genomic holds add unprecedented detail, frequently falls short potential.
Language: Английский
Citations
97
Evolution Letters, Journal Year: 2017, Volume and Issue: 1(3), P. 138 - 154
Published: June 14, 2017
Mechanisms that suppress recombination are known to help maintain species barriers by preventing the breakup of coadapted gene combinations. The sympatric butterfly
Language: Английский
Citations
96
PLoS Genetics, Journal Year: 2018, Volume and Issue: 14(11), P. e1007796 - e1007796
Published: Nov. 13, 2018
When environments change, populations may adapt surprisingly fast, repeatedly and even at microgeographic scales. There is increasing evidence that such cases of rapid parallel evolution are fueled by standing genetic variation, but the source this variation remains poorly understood. In saltmarsh beetle Pogonus chalceus, short-winged 'tidal' long-winged 'seasonal' ecotypes have diverged in response to contrasting hydrological regimes can be found along Atlantic European coast. By analyzing genomic across beetles' distribution, we reveal alleles selected tidal ecotype spread genome evolved during a singular and, likely, geographically isolated divergence event, within last 190 Kya. Due subsequent admixture, ancient differentially currently polymorphic most its range, which could potentially allow for fast one from small number random individuals, as low 5 15, population other ecotype. Our results suggest ecological result two different time frames: past, followed repeated selection on same divergently after admixture. These findings highlight importance an allopatric event driving rate direction contemporary under gene flow. This mechanism driven periods geographic isolation imposed large-scale environmental changes glacial cycles.
Language: Английский
Citations
90
PLoS Biology, Journal Year: 2020, Volume and Issue: 18(2), P. e3000597 - e3000597
Published: Feb. 6, 2020
Natural selection leaves distinct signatures in the genome that can reveal targets and history of adaptive evolution. By analysing high-coverage sequence data from 4 major colour pattern loci sampled nearly 600 individuals 53 populations, we show pervasive on wing patterns Heliconius radiation. The strongest correspond to with greatest phenotypic effects, consistent visual by predators, are found geographically restricted distributions. These recent sweeps similar between co-mimics indicate turn-over events despite strong stabilising selection. Using simulations, compare sweep expected under classic hard those resulting introgression, an important aspect mimicry evolution butterflies. Simulated recipient populations a 'volcano' peaks increased genetic diversity around selected target, characteristic introgressed variation some populations. Our genomic surprisingly dynamic co-evolution this
Language: Английский
Citations
81
Current Biology, Journal Year: 2020, Volume and Issue: 30(2), P. 304 - 311.e4
Published: Jan. 1, 2020
Language: Английский
Citations
73