Characterizing the rod pathway in cone-dominant thirteen-lined ground squirrels DOI Creative Commons
Riley Ferguson, Kiyoharu J. Miyagishima, Francisco M. Nadal‐Nicolás

et al.

Frontiers in Ophthalmology, Journal Year: 2023, Volume and Issue: 3

Published: Nov. 16, 2023

AII-amacrine cells (AIIs) are widely accepted as a critical element of scotopic pathways mediating night vision in the mammalian retina and have been well-characterized rod-dominant mice, rabbits, non-human primates. The rod pathway is characteristic all eyes, however, anatomic physiologic role AIIs cone dominant thirteen-lined ground squirrels (TLGS) limited. Here, we employed both immunohistochemistry electrophysiological approaches to investigate morphology functional aspects TLGS. In TLGS retinas examined, putative were calretinin-positive exhibited connections bipolar with decreased cell density expanded arborization. Notably, retained each other via gap junctions labeled Connexin36. Comparisons between single photoreceptor recordings full-field electroretinograms revealed ERG responses mediated by rods cones. Thus, components conserved signals traverse these cone-dominant animals. sparsely populated, matching diminished populations compared species. infrequent distribution lateral spacing AII’s indicate that they probably do not play significant signaling encode information at finer spatial scale. This contrasts mouse retina, where significantly contribute pathways. Therefore, original function likely ‘rod’ amacrine cell, its might be an adaptive feature stemming from dominance.

Language: Английский

The vertebrate retina: a window into the evolution of computation in the brain DOI Creative Commons
Tom Baden

Current Opinion in Behavioral Sciences, Journal Year: 2024, Volume and Issue: 57, P. 101391 - 101391

Published: April 17, 2024

Animal brains are probably the most complex computational machines on our planet, and like everything in biology, they product of evolution. Advances developmental palaeobiology have been expanding general understanding how nervous systems can change at a molecular structural level. However, these changes translate into altered function — that is, 'computation' remains comparatively sparsely explored. What, concretely, does it mean for neuronal computation when neurons their morphology connectivity, new appear or old ones disappear, transmitter slowly modified over many generations? And evolution use possible knobs dials to constantly tune give rise amazing diversity animal behaviours we see today? Addressing major gaps benefits from choosing suitable model system. Here, I present vertebrate retina as one perhaps unusually promising candidate. The is ancient displays highly conserved core organisational principles across entire lineage, alongside myriad adjustments extant species were shaped by history visual ecology. Moreover, logic readily interrogated experimentally, existing retinal circuits handful serve an anchor exploring circuit adaptations tree life, fish deep aphotic zone oceans eagles soaring high up sky.

Language: Английский

Citations

6
Molecular characterization of the sea lamprey retina illuminates the evolutionary origin of retinal cell types DOI Creative Commons
Junqiang Wang, Lin Zhang, Martina Cavallini

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 30, 2024

The lamprey, a primitive jawless vertebrate whose ancestors diverged from all other vertebrates over 500 million years ago, offers unique window into the ancient formation of retina. Using single-cell RNA-sequencing, we characterize retinal cell types in lamprey and compare them to those mouse, chicken, zebrafish. We find six classes 74 distinct types, many shared with species. conservation indicates their emergence early evolution, highlighting primordial designs circuits for rod pathway, ON-OFF discrimination, direction selectivity. diversification amacrine some ganglion appears, however, be lamprey. further infer genetic regulators specifying identify ancestral regulatory elements across species, noting decreased cells. Altogether, our characterization retina illuminates evolutionary origin visual processing

Language: Английский

Citations

5
Evolution of rod bipolar cells and rod vision DOI Open Access
Rikard Frederiksen, Yi‐Rong Peng, Alapakkam P. Sampath

et al.

The Journal of Physiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Abstract Bipolar cells are vertebrate retinal interneurons conveying signals from rod and cone photoreceptors to amacrine ganglion cells. found in all vertebrates have many structural molecular affinities with photoreceptors; they probably appeared very early during evolution conjunction progenitors. There two types of bipolar cells, responding central illumination depolarization (ON) or hyperpolarization (OFF). In most species, conveyed specialized which sum rods facilitate detection at the visual threshold. Lamprey, diverged other late Cambrian, both ON OFF but mammals only Rod output neurons indirectly via AII (or A2) synapse onto bipolar‐cells then These findings raise question when were lost. Because physiological recordings been made cartilaginous fishes (dogfish) urodeles (salamanders), their circuits must retained progenitors least until Devonian. Recent evidence showing that zebrafish retina processes similar those indicates lost twice. The sole utilization may provided a selective advantage increased signal‐to‐noise discrimination near image Key points Rods cones similarities output. can be either (centre depolarizing) hyperpolarizing) dominant. 500 million years ago, each We argue this configuration separate rod/cone bipolar‐cell pathways is representative vertebrates. bipolars persisted amphibians Devonian, teleost convey cell. different lineages evolution, increase vision.

Language: Английский

Citations

0
Nanophysiology Approach Reveals Diversity in Calcium Microdomains across Zebrafish Retinal Bipolar Ribbon Synapses DOI Open Access
Nirujan Rameshkumar, Abhishek P Shrestha, Johane M. Boff

et al.

Published: March 12, 2025

Rapid and high local calcium (Ca 2+ ) signals are essential for triggering neurotransmitter release from presynaptic terminals. In specialized bipolar ribbon synapses of the retina, these Ca control multiple processes, including priming, docking, translocation vesicles on before exocytosis, endocytosis, replenishment release-ready to fusion sites sustained neurotransmission. However, our knowledge about along axis active zone is limited. Here, we used fast confocal quantitative dual-color ratiometric line-scan imaging a fluorescently labeled binding peptide indicators monitor spatial temporal aspects transients individual zones in zebrafish retinal rod cells (RBCs). We observed that transient elicited much greater fluorescence amplitude when indicator was conjugated ribeye-binding than using soluble indicator, estimated levels at exceeded 26 μM response 10-millisecond stimulus, as measured by ribbon-bound low-affinity indicator. Our modeling diffusion buffering consistent with this estimate provides detailed view spatiotemporal [Ca ] dynamics near ribbon. Importantly, data demonstrates may vary between ribbons different RBCs within same cells. The variation correlated size, which turn correlates extent, serial electron microscopy new information heterogeneity shape, area contact plasma membrane.

Language: Английский

Citations

0
Nanophysiology Approach Reveals Diversity in Calcium Microdomains across Zebrafish Retinal Bipolar Ribbon Synapses DOI Open Access
Nirujan Rameshkumar, Abhishek P Shrestha, Johane M. Boff

et al.

Published: March 12, 2025

Rapid and high local calcium (Ca 2+ ) signals are essential for triggering neurotransmitter release from presynaptic terminals. In specialized bipolar ribbon synapses of the retina, these Ca control multiple processes, including priming, docking, translocation vesicles on before exocytosis, endocytosis, replenishment release-ready to fusion sites sustained neurotransmission. However, our knowledge about along axis active zone is limited. Here, we used fast confocal quantitative dual-color ratiometric line-scan imaging a fluorescently labeled binding peptide indicators monitor spatial temporal aspects transients individual zones in zebrafish retinal rod cells (RBCs). We observed that transient elicited much greater fluorescence amplitude when indicator was conjugated ribeye-binding than using soluble indicator, estimated levels at exceeded 26 μM response 10-millisecond stimulus, as measured by ribbon-bound low-affinity indicator. Our modeling diffusion buffering consistent with this estimate provides detailed view spatiotemporal [Ca ] dynamics near ribbon. Importantly, data demonstrates may vary between ribbons different RBCs within same cells. The variation correlated size, which turn correlates extent, serial electron microscopy new information heterogeneity shape, area contact plasma membrane.

Language: Английский

Citations

0
tet2 and tet3 regulate cell fate specification and differentiation events during retinal development DOI Creative Commons

Shea A Heilman,

Hannah Schriever, Dennis Kostka

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 26, 2025

Tet family methylcytosine dioxygenases recognize and oxidize 5-methyl-cytosine (5mC) to 5-hydroxymethylcytosine (5hmC). Previous work demonstrated the requirement for 5hmC during zebrafish retinogenesis. tet2−/−;tet3−/− mutants possessed defects in formation of differentiated retinal neurons, but mechanisms underlying these are unknown. Here, we leveraged scRNAseq technologies better understand cell type-specific deficits molecular signatures phenotype. Our results identified retinae that included delayed specification several types, reduced maturity across late-stage cones, expansions immature subpopulations horizontal bipolar cells, altered biases subtype fates at late differentiation stages. Together, data highlight critical role tet2 tet3 play as regulators fate terminal events development.

Language: Английский

Citations

0
Evolution: Non-linear spatial integration for high-acuity vision DOI
Takeshi Yoshimatsu

Current Biology, Journal Year: 2025, Volume and Issue: 35(9), P. R347 - R349

Published: May 1, 2025

Language: Английский

Citations

0
On analogies in vertebrate and insect visual systems DOI
Ryosuke Tanaka, Rubén Portugues

Nature reviews. Neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: May 23, 2025

Language: Английский

Citations

0
New twists in the evolution of retinal direction selectivity DOI Creative Commons
Takeshi Yoshimatsu, Tom Baden

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(2), P. e3002538 - e3002538

Published: Feb. 29, 2024

In mammals, starburst amacrine cells are centrally involved in motion vision and a new study PLOS Biology, by Yan colleagues finds that zebrafish have them, too. They coexist with second pair of starburst-like neurons, but neither appears to be strongly selective.

Language: Английский

Citations

1
Nanophysiology Approach Revealed Diversity in Calcium Microdomains in Zebrafish Retinal Bipolar Ribbon Synapses DOI Open Access
Nirujan Rameshkumar, Abhishek P Shrestha, Johane M. Boff

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Rapid and high local calcium (Ca 2+ ) signals are essential for triggering neurotransmitter release from presynaptic terminals. In specialized bipolar ribbon synapses of the retina, these Ca control multiple processes, including priming, docking, translocation vesicles on before exocytosis, endocytosis, replenishment release-ready to fusion sites sustained neurotransmission. However, our knowledge about along axis active zone is limited. Here, we used fast confocal quantitative dual-color ratiometric line-scan imaging a fluorescently labeled binding peptide indicators monitor spatial temporal aspects transients individual zones in zebrafish retinal rod cells (RBCs). We observed that transient elicited much greater fluorescence amplitude when indicator was conjugated ribeye-binding than using soluble indicator, estimated levels at exceeded 26 μM response 10-millisecond stimulus, as measured by ribbon-bound low-affinity indicator. Our modeling diffusion buffering consistent with this estimate provides detailed view spatiotemporal [Ca ] dynamics near ribbon. Importantly, data demonstrates may vary between ribbons different RBCs within same cells. The variation correlated size, which turn correlates extent, serial electron microscopy new information heterogeneity shape, area contact plasma membrane.

Language: Английский

Citations

0