Codon bias, nucleotide selection, and genome size predict in situ bacterial growth rate and transcription in rewetted soil

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(3)

Published: Jan. 13, 2025

In soils, the first rain after a prolonged dry period represents major pulse event impacting soil microbial community function, yet we lack full understanding of genomic traits associated with response to rewetting. Genomic such as codon usage bias and genome size have been linked bacterial growth in soils—however, often through measurements culture. Here, used metagenome-assembled genomes (MAGs) 18 O-water stable isotope probing metatranscriptomics track transcription microorganisms over one week following rewetting grassland soil. We found that ribosomal protein genes was strongest predictor rate. also higher rates bacteria smaller genomes, suggesting reduced enables faster pulses bacteria. Faster transcriptional upregulation high increased nucleotide skew. several these relationships existed within phyla, indicating associations between activity could be generalized characteristics Finally, publicly available metagenomes assess distribution across pH gradient communities soils—which are more water limited driven—have their genes. Together, results provide evidence affect during pose potential fitness advantage for where nutrient availability episodic.

Language: Английский

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Carbon and energy utilization in microbial cell extracts from soil

European Journal of Soil Biology, Journal Year: 2025, Volume and Issue: 124, P. 103713 - 103713

Published: Jan. 30, 2025

Language: Английский

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Simulated Climate Change Enhances Microbial Drought Resilience in Ethiopian Croplands but Not Forests

Global Change Biology, Journal Year: 2025, Volume and Issue: 31(3)

Published: March 1, 2025

Climate change and land-use represent a dual threat to terrestrial ecosystem functioning. In the tropics, forest conversion agriculture is occurring alongside warming more pronounced periods of drought. Rainfall after drought induces enormous dynamics in microbial growth (potential soil carbon storage) respiration (determining loss), affecting budget. We investigated how legacies affected functional (growth respiration) structural (16S ITS amplicon) responses Rain shelters open-top chambers (OTCs) were used simulate tropical cropland sites Ethiopia. reduced moisture by up 25 vol%, with bigger effect forest, while OTCs increased temperature 6°C also but had no clear forest. Soils from these field treatments then exposed standardized cycle test community traits been shaped different climate legacies. Microbial started increasing immediately rewetting all soils, reflecting resilient response indicating that communities perceived perturbation as relatively mild. Fungi recovered faster than bacteria, recovery fungal was generally accelerated soils legacy functions structures both responsive particularly enhanced not higher efficiency rewetting. Together, results suggest contrasting feedbacks determined land use, where croplands will be associated mitigated losses microorganisms future cycles drought, compared forests reservoirs remain sensitive.

Language: Английский

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Enhancing the anti-clogging performance of emitters in drip irrigation systems based on Bacillus subtilis strain DSM 10 and controlled replacement cycles

Biosystems Engineering, Journal Year: 2025, Volume and Issue: 252, P. 159 - 168

Published: March 27, 2025

Language: Английский

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Enhancement of soil phosphorus mineralization and phosphorus availability by labile carbon in organic amendments through boosting copiotrophic phosphatase-producing bacteria

Plant and Soil, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Language: Английский

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