Designing Hydrogels for 3D Cell Culture Using Dynamic Covalent Crosslinking DOI
Muhammad Rizwan, Alexander E. G. Baker, Molly S. Shoichet

et al.

Advanced Healthcare Materials, Journal Year: 2021, Volume and Issue: 10(12)

Published: May 14, 2021

Designing simple biomaterials to replicate the biochemical and mechanical properties of tissues is an ongoing challenge in tissue engineering. For several decades, new have been engineered using cytocompatible chemical reactions spontaneous ligations via click chemistries generate scaffolds water swollen polymer networks, known as hydrogels, with tunable properties. However, most these materials are static nature, providing only macroscopic tunability scaffold mechanics, do not reflect dynamic environment natural extracellular microenvironment. more complex applications such organoids or co-culture systems, there remain opportunities investigate cells that locally remodel change physicochemical within matrices. In this review, advanced where covalent chemistry used produce stable 3D cell culture models high-resolution constructs for both vitro vivo applications, discussed. The implications on viscoelastic summarized, case studies critically analyzed, further improve performance engineering

Language: Английский

Interplay between mechanics and signalling in regulating cell fate DOI
Henry De Belly, Ewa K. Paluch, Kevin J. Chalut

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2022, Volume and Issue: 23(7), P. 465 - 480

Published: April 1, 2022

Language: Английский

Citations

148
Nanoengineered Osteoinductive Bioink for 3D Bioprinting Bone Tissue DOI
David Chimene, Logan E. Miller, Lauren Cross

et al.

ACS Applied Materials & Interfaces, Journal Year: 2020, Volume and Issue: 12(14), P. 15976 - 15988

Published: Feb. 24, 2020

Bioprinting is an emerging additive manufacturing approach to the fabrication of patient-specific, implantable three-dimensional (3D) constructs for regenerative medicine. However, developing cell-compatible bioinks with high printability, structural stability, biodegradability, and bioactive characteristics still a primary challenge translating 3D bioprinting technology preclinical clinal models. To overcome this challenge, we developed nanoengineered ionic covalent entanglement (NICE) bioink formulation bone bioprinting. The NICE allow precise control over mechanical properties, degradation characteristics, enabling custom mechanically resilient, cellularized structures. We demonstrate cell-induced remodeling bioprinted scaffolds 60 days, demonstrating deposition nascent extracellular matrix proteins. Interestingly, induce endochondral differentiation encapsulated human mesenchymal stem cells (hMSCs) in absence osteoinducing agent. Using next-generation transcriptome sequencing (RNA-seq) technology, establish role nanosilicates, component bioink, stimulate at level. Overall, osteoinductive has ability formation osteo-related mineralized by hMSCs growth factor-free conditions. Furthermore, fabricate repair craniomaxillofacial defects. envision development toward realistic clinical process patient-specific tissue

Language: Английский

Citations

142
The living interface between synthetic biology and biomaterial design DOI
Allen P. Liu, Eric A. Appel, Paul D. Ashby

et al.

Nature Materials, Journal Year: 2022, Volume and Issue: 21(4), P. 390 - 397

Published: March 31, 2022

Language: Английский

Citations

134
Viscoelastic Cell Microenvironment: Hydrogel‐Based Strategy for Recapitulating Dynamic ECM Mechanics DOI
Yufei Ma,

Ting Han,

Qinxuan Yang

et al.

Advanced Functional Materials, Journal Year: 2021, Volume and Issue: 31(24)

Published: March 31, 2021

Abstract The native extracellular matrix (ECM) generally exhibits dynamic mechanical properties and displays time‐dependent responses to deformation or loading, in terms of viscoelastic behaviors (e.g., stress relaxation creep). Viscoelasticity the ECM plays a critical role development, homeostasis, tissue regeneration, its implication disease progression has also been recognized recently. Hydrogels with tunable hold great promise recapitulate such mechanics found ECM, which have recently used regulate cell behavior guide fate. Here importance viscoelasticity is first highlighted, molecular mechanisms hydrogel are summarized, characterization techniques at macroscale microscale reviewed. Then, recent advances developing novel hydrogels through varying crosslinking strategies, engineering microenvironment substantial effects on fate described, underlying mechanobiology subsequently discussed. Finally, ongoing challenges future perspectives design modulation cellular proposed.

Language: Английский

Citations

130
Designing Hydrogels for 3D Cell Culture Using Dynamic Covalent Crosslinking DOI
Muhammad Rizwan, Alexander E. G. Baker, Molly S. Shoichet

et al.

Advanced Healthcare Materials, Journal Year: 2021, Volume and Issue: 10(12)

Published: May 14, 2021

Designing simple biomaterials to replicate the biochemical and mechanical properties of tissues is an ongoing challenge in tissue engineering. For several decades, new have been engineered using cytocompatible chemical reactions spontaneous ligations via click chemistries generate scaffolds water swollen polymer networks, known as hydrogels, with tunable properties. However, most these materials are static nature, providing only macroscopic tunability scaffold mechanics, do not reflect dynamic environment natural extracellular microenvironment. more complex applications such organoids or co-culture systems, there remain opportunities investigate cells that locally remodel change physicochemical within matrices. In this review, advanced where covalent chemistry used produce stable 3D cell culture models high-resolution constructs for both vitro vivo applications, discussed. The implications on viscoelastic summarized, case studies critically analyzed, further improve performance engineering

Language: Английский

Citations

121