Calcium silicate nanowires-containing multicellular bioinks for 3D bioprinting of neural-bone constructs

Nano Today, Journal Year: 2022, Volume and Issue: 46, P. 101584 - 101584

Published: Aug. 9, 2022

Language: Английский

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3D cancer models: One step closer to in vitro human studies

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: April 11, 2023

Cancer immunotherapy is the great breakthrough in cancer treatment as it displayed prolonged progression-free survival over conventional therapies, yet, to date, only a minority of patients. In order broad clinical applicability some roadblocks need be overcome, first among all lack preclinical models that faithfully depict local tumor microenvironment (TME), which known dramatically affect disease onset, progression and response therapy. this review, we provide reader with detailed overview current 3D developed mimick complexity dynamics TME, focus on understanding why TME major target anticancer We highlight advantages translational potentials spheroids, organoids immune Tumor-on-a-Chip modeling therapeutic response, while outlining pending challenges limitations. Thinking forward, possibility integrate know-hows micro-engineers, immunologists, pharmaceutical researchers bioinformaticians meet needs clinicians interested using these platforms high fidelity for patient-tailored drug discovery.

Language: Английский

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The current state and future of T-cell exhaustion research

Oxford Open Immunology, Journal Year: 2023, Volume and Issue: 4(1)

Published: Jan. 1, 2023

Abstract ‘Exhaustion’ is a term used to describe state of native and redirected T-cell hypo-responsiveness resulting from persistent antigen exposure during chronic viral infections or cancer. Although well-established phenotype across mice humans, exhaustion at the molecular level remains poorly defined inconsistent literature. This is, in part, due an overreliance on surface receptors define these cells explain exhaustive behaviours, incomplete understanding how arises, lack clarity over whether same contexts, e.g. versus With development systems-based genetic approaches such as single-cell RNA-seq CRISPR screens applied vivo data, we are moving closer consensus view exhaustion, although it arises challenging given difficulty manipulating setting. Accordingly, producing studying exhausted T-cells ex burgeoning, allowing experiments be conducted scale up with high throughput. Here, first review what currently known about it’s being studied. We then discuss improvements their method isolation/production examining impact different microenvironmental signals cell interactions have now become active area research. Finally, future holds for analysis this physiological condition and, diversity ways which generated, propose adoption unified approach clearly defining using set metabolic-, epigenetic-, transcriptional-, activation-based phenotypic markers, that call ‘M.E.T.A’.

Language: Английский

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In vitro models to study human gut-microbiota interactions: Applications, advances, and limitations

Microbiological Research, Journal Year: 2023, Volume and Issue: 270, P. 127336 - 127336

Published: Feb. 16, 2023

Language: Английский

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Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity

Clinical Epigenetics, Journal Year: 2023, Volume and Issue: 15(1)

Published: Oct. 11, 2023

Previous studies have traditionally attributed the initiation of cancer cells to genetic mutations, considering them as fundamental drivers carcinogenesis. However, recent research has shed light on crucial role epigenomic alterations in various cell types present within tumor microenvironment, suggesting their potential contribution formation and progression. Despite these significant findings, progress understanding epigenetic mechanisms regulating heterogeneity been impeded over past few years due lack appropriate technical tools methodologies.The emergence single-cell sequencing enhanced our governing by revealing distinct layers individual (chromatin accessibility, DNA/RNA methylation, histone modifications, nucleosome localization) diverse omics (transcriptomics, genomics, multi-omics) at level. These technologies provide us with new insights into molecular basis intratumoral help uncover key events driving development.This paper provides a comprehensive review emerging analytical experimental approaches omics, focusing specifically epigenomics. capture integrate multiple dimensions cells, thereby features. Additionally, this outlines future trends current limitations.

Language: Английский

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