Evaluation of anti-biofilm activity of acidic amino acids and synergy with ciprofloxacin on Staphylococcus aureus biofilms

Scientific Reports, Journal Year: 2020, Volume and Issue: 10(1)

Published: June 2, 2020

Abstract Acidic amino acids, aspartic acid (Asp) and glutamic (Glu) can enhance the solubility of many poorly soluble drugs including ciprofloxacin (Cip). One mechanisms resistance within a biofilm is retardation drug diffusion due to poor penetration across matrix. To overcome this challenge, work set investigate novel counter ion approach with acidic which we hypothesised will disrupt matrix as well simultaneously improve effectiveness. The anti-biofilm activity D-Asp D-Glu was studied on Staphylococcus aureus biofilms. Synergistic effect combining D-amino acids Cip also investigated strategy anti-microbial in these Interestingly at equimolar combinations, were able significantly disperse (at 20 mM 40 mM) established biofilms inhibit 10 mM, new formation absence an antibiotic. Moreover, our study confirmed L-amino exhibit activity. synergistic observed lower concentration ranges (<40 <90.54 µM, respectively), resulted 96.89% (inhibition) 97.60% (dispersal) reduction CFU exposure acids. Confocal imaging indicated that honeycomb-like extracellular DNA (eDNA) meshwork whilst preventing its formation.

Language: Английский

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Targeting Bacterial Biofilms by the Green Tea Polyphenol EGCG

Molecules, Journal Year: 2019, Volume and Issue: 24(13), P. 2403 - 2403

Published: June 29, 2019

Bacterial biofilms are multicellular aggregates in which cells embedded an extracellular matrix of self-produced biopolymers. Being refractory to antibiotic treatment and host immune systems, involved most chronic infections, anti-biofilm agents being searched for urgently. Epigallocatechin-3-gallate (EGCG) was recently shown act against by strongly interfering with the assembly amyloid fibres production phosphoethanolamin-modified cellulose fibrils. Mechanistically, this includes a direct inhibition fibre assembly, but also triggers cell envelope stress response that down-regulates synthesis these widely occurring biofilm polymers. Based on its anti-amyloidogenic properties, EGCG seems useful cariogenesis or wound infection. However, inefficient may even sometimes promote rely other types polymers, suggesting searching ‘magic bullet’ is unrealistic goal. Combining molecular ecophysiological aspects review illustrates why plants control formation their surfaces producing compounds such as EGCG. These not only helpful combating certain infections seem effective toxic amyloids associated neuropathological diseases.

Language: Английский

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Biofilm Structure Promotes Coexistence of Phage-Resistant and Phage-Susceptible Bacteria

mSystems, Journal Year: 2020, Volume and Issue: 5(3)

Published: June 26, 2020

In the natural environment, bacteria most often live in communities bound to one another by secreted adhesives. These communities, or biofilms, play a central role biogeochemical cycling, microbiome functioning, wastewater treatment, and disease. Wherever there are bacteria, also viruses that attack them, called phages. Interactions between phages likely occur ubiquitously biofilms. We show here, using simulations experiments, biofilms will conditions allow phage-susceptible be protected from phage exposure, if they growing alongside other cells resistant. This result has implications for fundamental ecology of phage-bacteria interactions, as well development phage-based antimicrobial therapeutics.

Language: Английский

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Understanding the Complex Phage-Host Interactions in Biofilm Communities

Annual Review of Virology, Journal Year: 2021, Volume and Issue: 8(1), P. 73 - 94

Published: June 29, 2021

Bacteriophages and bacterial biofilms are widely present in natural environments, a fact that has accelerated the evolution of phages their hosts these particular niches. Phage-host interactions biofilm communities rather complex, where not always merely predators but also can establish symbiotic relationships induce strengthen biofilms. In this review we provide an overview main features affecting phage-biofilm as well currently available methods studying interactions. addition, address applications for control different contexts.

Language: Английский

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Biological foundations of successful bacteriophage therapy

EMBO Molecular Medicine, Journal Year: 2022, Volume and Issue: 14(7)

Published: May 27, 2022

Language: Английский

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