Analysis of nearly 3000 archaeal genomes from terrestrial geothermal springs sheds light on interconnected biogeochemical processes

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 14, 2024

Abstract Terrestrial geothermal springs are physicochemically diverse and host abundant populations of Archaea. However, the diversity, functionality, geological influences these Archaea not well understood. Here we explore genomic diversity in 152 metagenomes from 48 Tengchong, China, collected 2016 to 2021. Our dataset is comprised 2949 archaeal metagenome-assembled genomes spanning 12 phyla 392 newly identified species, which increases known species by ~48.6%. The structures potential functions communities strongly influenced temperature pH, with high-temperature acidic alkaline favoring abundance over Bacteria. Genome-resolved metagenomics metatranscriptomics provide insights into ecological niches their roles carbon, sulfur, nitrogen, hydrogen metabolism. Furthermore, our findings illustrate interplay competition cooperation among biogeochemical cycles, possibly arising overlapping functional metabolic handoffs. Taken together, study expands inhabiting provides a foundation for more incisive processes mediated ecosystems.

Language: Английский

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Microbial ecology of the deep terrestrial subsurface

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

The terrestrial subsurface hosts microbial communities that, collectively, are predicted to comprise as many cells global surface soils. Although initially thought be associated with deposited organic matter, deep supported by chemolithoautotrophic primary production, hydrogen serving an important source of electrons. Despite recent progress, relatively little is known about the compared more commonly studied environments. Understanding composition and factors that influence them importance because human-associated activities including long-term storage used nuclear fuel, carbon capture, for use energy vector. In addition identifying microorganisms, research focuses on roles microorganisms in communities, well elucidating myriad interactions-syntrophic, episymbiotic, viral-that occur among community members. years, entirely new groups (i.e. candidate phyla radiation bacteria Diapherotrites, Parvarchaeota, Aenigmarchaeota, Nanoloarchaeota, Nanoarchaeota archaea) have been discovered environments, suggesting much remains unknown this biosphere. This review explores historical context ecology highlights discoveries shape current ecological understanding poorly explored habitat. Additionally, we highlight need multifaceted experimental approaches observe phenomena such cryptic cycles, complex interactions, episymbiosis, which may not apparent when using single isolation, but nonetheless critical advancing our

Language: Английский

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Minimal and hybrid hydrogenases are active from archaea

Cell, Journal Year: 2024, Volume and Issue: 187(13), P. 3357 - 3372.e19

Published: June 1, 2024

Microbial hydrogen (H2) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted bacteria eukaryotes. Here, we show that anaerobic archaea encode diverse, active, ancient lineages through combining analysis existing new genomes with extensive biochemical experiments. are encoded by nine archaeal phyla expressed H2-producing Asgard archaeon cultures. We report an ultraminimal in DPANN binds catalytic H-cluster produces H2. Moreover, identify characterize remarkable hybrid complexes formed fusion [NiFe] ten other orders. Phylogenetic structural modeling suggest a deep evolutionary history hydrogenases. These findings reveal metabolic adaptations archaea, streamlined H2 catalysts for biotechnological development, surprisingly intertwined between two major H2-metabolizing enzymes.

Language: Английский

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Differential impacts of water diversion and environmental factors on bacterial, archaeal, and fungal communities in the eastern route of the South-to-North water diversion project

Environment International, Journal Year: 2025, Volume and Issue: 195, P. 109280 - 109280

Published: Jan. 1, 2025

Water diversion projects effectively mitigate the uneven distribution of water resources but can also influence aquatic biodiversity and ecosystem functions. Despite their importance, impacts such on multi-domain microbial community dynamics underlying mechanisms remain poorly understood. Utilizing high-throughput sequencing, we investigated bacterial, archaeal, fungal along eastern route South-to-North project during both non-water period (NWDP) (WDP). Our findings revealed competitive exclusion effects among bacterial archaeal communities WDP, characterized by decreased species richness increased biomass, while biomass significantly declined. Distance-decay relationships suggested homogenization WDP. Robustness analyses reduced stability with primarily influencing stability, environmental factors had a greater impact communities. Stochastic processes, homogenizing dispersal drift, intensified for Notably, only functional diversity relative abundance chemoheterotrophic organic compound catabolic bacteria declined photoautotrophic bacteria. PLS-PM indicated that shaped assembly processes guilds, whereas This study enhances our understanding WDP underscores importance assessing direct resulting fluctuations.

Language: Английский

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Soil Candidate Phyla Radiation Bacteria Encode Components of Aerobic Metabolism and Co-occur with Nanoarchaea in the Rare Biosphere of Rhizosphere Grassland Communities

mSystems, Journal Year: 2021, Volume and Issue: 6(4)

Published: Aug. 17, 2021

Here, we investigated overlooked microbes in soil, candidate phyla radiation (CPR) bacteria and Diapherotrites, Parvarchaeota, Aenigmarchaeota, Nanoarchaeota, Nanohaloarchaeota (DPANN) archaea, by size fractionating small particles from an approach typically used for the recovery of viral metagenomes. Concentration these cells (<0.2 μm) allowed us to identify organisms as part rare soil biosphere sample genomes that were absent non-size-fractionated

Language: Английский

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