Current Opinion in Microbiology,
Journal Year:
2022,
Volume and Issue:
71, P. 102245 - 102245
Published: Dec. 10, 2022
Bacteriophages
are
as
ubiquitous
their
bacterial
hosts
and
often
more
abundant.
Understanding
how
bacteriophages
control
host
populations
requires
a
number
of
different
approaches.
can
through
lysis,
drive
evolution
immunity
systems
infection,
provide
conduit
for
horizontal
gene
transfer
alter
metabolism
by
carriage
auxiliary
metabolic
genes.
quantifying
these
processes,
both
technological
developments
to
take
measurements
in
situ,
laboratory-based
studies
understand
mechanisms.
Technological
advances
have
allowed
quantification
the
infected
cells
revealing
far-lower
levels
than
expected.
observations
laboratory
conditions
relate
what
occurs
environment,
experimental
confirmation
predicted
function
phage
genes
from
environmental
omics
data,
remains
challenging.
Cell Reports,
Journal Year:
2023,
Volume and Issue:
42(7), P. 112672 - 112672
Published: June 21, 2023
Prokaryotic
adaptation
is
strongly
influenced
by
the
horizontal
acquisition
of
beneficial
traits
via
mobile
genetic
elements
(MGEs),
such
as
viruses/bacteriophages
and
plasmids.
However,
MGEs
can
also
impose
a
fitness
cost
due
to
their
often
parasitic
nature
differing
evolutionary
trajectories.
In
response,
prokaryotes
have
evolved
diverse
immune
mechanisms
against
MGEs.
Recently,
our
understanding
abundance
diversity
prokaryotic
systems
has
greatly
expanded.
These
defense
degrade
invading
material,
inhibit
genome
replication,
or
trigger
abortive
infection,
leading
population
protection.
this
review,
we
highlight
these
strategies,
focusing
on
most
recent
discoveries.
The
study
defenses
not
only
sheds
light
microbial
evolution
but
uncovers
novel
enzymatic
activities
with
promising
biotechnological
applications.
Nature Microbiology,
Journal Year:
2023,
Volume and Issue:
8(5), P. 946 - 957
Published: April 6, 2023
Abstract
Many
microbes
in
nature
reside
dense,
metabolically
interdependent
communities.
We
investigated
the
and
extent
of
microbe-virus
interactions
relation
to
microbial
density
syntrophy
by
examining
a
biomass
deep-sea
hydrothermal
mat.
Using
metagenomic
sequencing,
we
find
numerous
instances
where
phylogenetically
distant
(up
domain
level)
encode
CRISPR-based
immunity
against
same
viruses
Evidence
viral
with
hosts
cross-cutting
domains
is
particularly
striking
between
known
syntrophic
partners,
for
example
those
engaged
anaerobic
methanotrophy.
These
patterns
are
corroborated
proximity-ligation-based
(Hi-C)
inference.
Surveys
public
datasets
reveal
additional
interacting
across
diverse
ecosystems
harbour
biofilms.
propose
that
entry
particles
and/or
DNA
non-primary
host
cells
may
be
common
phenomenon
densely
populated
ecosystems,
eco-evolutionary
implications
CRISPR-mediated
inter-population
augmentation
resilience
viruses.
Cell Host & Microbe,
Journal Year:
2024,
Volume and Issue:
32(4), P. 557 - 572.e6
Published: Feb. 22, 2024
Bacterial
defense
against
phage
predation
involves
diverse
systems
acting
individually
and
concurrently,
yet
their
interactions
remain
poorly
understood.
We
investigated
>100
in
42,925
bacterial
genomes
identified
numerous
instances
of
non-random
co-occurrence
negative
association.
For
several
pairs
significantly
co-occurring
Escherichia
coli
strains,
we
demonstrate
synergistic
anti-phage
activity.
Notably,
Zorya
II
synergizes
with
Druantia
III
ietAS
systems,
while
tmn
exhibits
synergy
Gabija,
Septu
I,
PrrC.
co-opts
the
sensory
switch
ATPase
domain,
enhancing
Some
system
that
are
negatively
associated
E.
show
co-occur
other
taxa,
demonstrating
immune
repertoires
largely
shaped
by
selection
for
resistance
host-specific
phages
rather
than
epistasis.
Collectively,
these
findings
compatibility
between
allowing
bacteria
to
adopt
flexible
strategies
defense.
Science Advances,
Journal Year:
2024,
Volume and Issue:
10(8)
Published: Feb. 23, 2024
Prokaryotes
encode
multiple
distinct
anti-phage
defense
systems
in
their
genomes.
However,
the
impact
of
carrying
a
multitude
on
phage
resistance
remains
unclear,
especially
clinical
context.
Using
collection
antibiotic-resistant
strains
Pseudomonas
aeruginosa
and
broad
panel
phages,
we
demonstrate
that
contribute
substantially
to
defining
host
range
overall
scales
with
number
bacterial
genome.
We
show
many
individual
target
specific
genera
complementary
specificities
co-occur
P.
genomes
likely
provide
benefits
phage-diverse
environments.
Overall,
phage-resistant
phenotypes
at
least
19
exist
populations
clinical,
strains.
Frontiers in Cellular and Infection Microbiology,
Journal Year:
2024,
Volume and Issue:
14
Published: Jan. 31, 2024
Drug-resistant
Staphylococcus
aureus
stands
as
a
prominent
pathogen
in
nosocomial
and
community-acquired
infections,
capable
of
inciting
various
infections
at
different
sites
patients.
This
includes
bacteremia
(SaB),
which
exhibits
severe
infection
frequently
associated
with
significant
mortality
rate
approximately
25%.
In
the
absence
better
alternative
therapies,
antibiotics
is
still
main
approach
for
treating
infections.
However,
excessive
use
has,
turn,
led
to
an
increase
antimicrobial
resistance.
Hence,
it
imperative
that
new
strategies
are
developed
control
drug-resistant
S.
Bacteriophages
viruses
ability
infect
bacteria.
Bacteriophages,
were
used
treat
bacterial
before
advent
antibiotics,
but
subsequently
replaced
by
due
limited
theoretical
understanding
inefficient
preparation
processes
time.
Recently,
phages
have
attracted
attention
many
researchers
again
because
serious
problem
antibiotic
article
provides
comprehensive
overview
phage
biology,
animal
models,
diverse
clinical
case
treatments,
trials
context
therapy.
It
also
assesses
strengths
limitations
therapy
outlines
future
prospects
research
directions.
review
expected
offer
valuable
insights
engaged
phage-based
treatments
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Jan. 22, 2024
Abstract
Phage
satellites
are
bacterial
genetic
elements
that
co-opt
phage
machinery
for
their
own
dissemination.
Here
we
identify
a
family
of
satellites,
named
Phage-Inducible
Chromosomal
Minimalist
Islands
(PICMIs),
broadly
distributed
in
marine
bacteria
the
Vibrionaceae.
A
typical
PICMI
is
characterized
by
reduced
gene
content,
does
not
encode
genes
capsid
remodelling,
and
packages
its
DNA
as
concatemer.
PICMIs
integrate
host
genome
next
to
fis
regulator,
three
core
proteins
necessary
excision
replication.
dependent
on
virulent
particles
spread
other
bacteria,
protect
hosts
from
competitive
phages
without
interfering
with
helper
phage.
Thus,
our
work
broadens
understanding
narrows
down
minimal
number
functions
hijack
tailed
