A comparative study revealed hyperspectral imaging as a potential standardized tool for the analysis of cuticle tanning over insect development DOI Creative Commons
Virginie Lacotte, Elisa Dell’Aglio, Sergio Peignier

et al.

Heliyon, Journal Year: 2023, Volume and Issue: 9(3), P. e13962 - e13962

Published: Feb. 23, 2023

Cereal-feeding beetles are a major risk for cereal crop maintenance. Cereal weevils such as Sitophilus oryzae have symbiotic intracellular bacteria that provide essential aromatic amino acid to the host biosynthesis of their cuticle building blocks. Their is an important protective barrier against biotic and abiotic stresses, providing high resistance from insecticides. Quantitative optical methods specialized in insect analysis exist, but scope use repeatability results remain limited. Here, we investigated potential Hyperspectral Imaging (HSI) standardized tool. Based on HSI, acquired time series average reflectance profiles 400 1000 nm (with bacteria) aposymbiotic (without S. exposed different nutritional stresses. We assessed phenotypic changes under diets throughout development demonstrated agreement between HSI method classically used Red-Green-Blue analysis. Then, compared both technologies laboratory conditions highlighted assets develop simple, automated, This first study showing reliability feasibility changes.

Language: Английский

The genus Sodalis as a resource for understanding the multifaceted evolution of bacterial symbiosis in insects DOI
François Renoz, Hiroshi Arai, Inès Pons

et al.

Symbiosis, Journal Year: 2023, Volume and Issue: 92(2), P. 187 - 208

Published: Dec. 30, 2023

Language: Английский

Citations

7
Host range of naturally and artificially evolved symbiotic bacteria for a specific host insect DOI Creative Commons

Ryuga Sugiyama,

Minoru Moriyama, Ryuichi Koga

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(9)

Published: July 31, 2024

ABSTRACT Diverse insects are intimately associated with specific symbiotic bacteria, where host and symbiont integrated into an almost inseparable biological entity. These bacteria usually exhibit specificity, uncultivability, reduced genome size, other peculiar traits relevant to their lifestyle. How host-symbiont specificity is established at the very beginning of symbiosis interest but poorly understood. To gain insight evolutionary issue, we adopted experimental approach using recently developed model between stinkbug Plautia stali Escherichia coli . Based on laboratory evolution P. stali-E. mutualism, selected Δ cyaA mutant E. as artificial that has mutualism by a single mutation. In addition, natural cultivable relatively recent origin. were experimentally inoculated symbiont-deprived newborn nymphs diverse species. Strikingly, mutualistic was unable establish infection support growth survival all species except for , uncovering can be early stage evolution. Meanwhile, able several in addition unveiling broader range evolved nature. these findings, discuss what factors establishment symbiosis. IMPORTANCE does emerge symbiosis? This question difficult address because it generally directly observe onset However, development approaches brought about breakthrough. Here tackled this issue created Pantoea symbiont, which both We replaced essential stinkbugs symbionts evaluated whether host, heterospecific hosts. showed strict whereas capable stinkbugs, provide how stages

Language: Английский

Citations

2
Genome dynamics across the evolutionary transition to endosymbiosis DOI Creative Commons
Stefanos Siozios, Pol Nadal‐Jimenez, Tal Azagi

et al.

Current Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Endosymbiosis-where a microbe lives and replicates within host-is an important contributor to organismal function that has accelerated evolutionary innovations catalyzed the evolution of complex life. The processes associated with transitions endosymbiosis, however, are poorly understood. Here, we leverage wide diversity host-associated lifestyles genus Arsenophonus reveal occur during transition vertically transmitted endosymbiotic lifestyle from strains maintained solely by horizontal (infectious) transmission. We compared genomes 38 spanning diverse horizontally pathogens obligate interdependent endosymbionts. Among culturable strains, observed those vertical transmission had larger genome sizes than closely related transmitting counterparts, consistent innovation rapid gain new functions. Increased size was consequence prophage plasmid acquisition, including cargo type III effectors, alongside concomitant loss CRISPR-Cas defense systems, enabling mobile genetic element expansion. Persistent endosymbiosis also VI secretion, which hypothesize be reduced microbe-microbe competition. Thereafter, strict inheritance expected relaxation purifying selection, gene pseudogenization, metabolic degradation, reduction. argue phage predation in niches drives systems driving expansion upon adoption This remodeling enables transfer-mediated precedes reductive traditionally adaptation endosymbiosis.

Language: Английский

Citations

2
Pantoea bathycoeliae sp. nov and Sodalis sp. are core gut microbiome symbionts of the two-spotted stink bug DOI Creative Commons
Arista Fourie, Stephanus N. Venter, Bernard Slippers

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 30, 2023

Stink bug species (Pentatomoidea superfamily) have developed an interdependence with obligate bacterial gut symbionts in specialized midgut crypts (M4 sub-region). Species of the Enterobacteriaceae family (predominantly Pantoea) are vertically transferred to their offspring and provide nutrients that cannot be obtained from plant sap food sources. However, bacteria other compartments stink bugs rarely been investigated. The two-spotted bug, Bathycoelia distincta, is a serious pest macadamias South Africa. Nothing currently known regarding its microbiome or how between insect generations. In this study, consistency B. distincta across geographic locations life stages was determined 16S rRNA metabarcoding, considering both M4 compartments. A novel Pantoea found primary symbiont offspring. had low diversity genera varied populations but Sodalis prominent all populations. Sequence data compartment were used produce high-quality metagenome-assembled genomes (MAGs) for species. Functional analyses suggested similar role nutrient provision host, yet also unique metabolites produced by each sp. additional traits, such as secretion systems, likely allowed it establish itself host. described bathycoeliae nov based on rules SeqCode.

Language: Английский

Citations

6
A comparative study revealed hyperspectral imaging as a potential standardized tool for the analysis of cuticle tanning over insect development DOI Creative Commons
Virginie Lacotte, Elisa Dell’Aglio, Sergio Peignier

et al.

Heliyon, Journal Year: 2023, Volume and Issue: 9(3), P. e13962 - e13962

Published: Feb. 23, 2023

Cereal-feeding beetles are a major risk for cereal crop maintenance. Cereal weevils such as Sitophilus oryzae have symbiotic intracellular bacteria that provide essential aromatic amino acid to the host biosynthesis of their cuticle building blocks. Their is an important protective barrier against biotic and abiotic stresses, providing high resistance from insecticides. Quantitative optical methods specialized in insect analysis exist, but scope use repeatability results remain limited. Here, we investigated potential Hyperspectral Imaging (HSI) standardized tool. Based on HSI, acquired time series average reflectance profiles 400 1000 nm (with bacteria) aposymbiotic (without S. exposed different nutritional stresses. We assessed phenotypic changes under diets throughout development demonstrated agreement between HSI method classically used Red-Green-Blue analysis. Then, compared both technologies laboratory conditions highlighted assets develop simple, automated, This first study showing reliability feasibility changes.

Language: Английский

Citations

5