Identification of housekeeping gene for future studies exploring effects of cryopreservation on gene expression in shrimp

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 1, 2025

Abstract Few studies have investigated the subcellular effects of low temperature on gene expression in shrimp and most other crustaceans. Before analysis is conducted, suitable housekeeping genes (HKGs) must be confirmed to account for differences reverse transcription process efficiency among samples. Thus, this study aimed verify five frequently used HKGs, namely 18S ribosomal RNA (18S rRNA), ATPase, histone 3, β-actin, glyceraldehyde 3-phosphate dehydrogenase (gapdh) use experiments assessing molecular-scale cryopreservation coral banded ( Stenopus hispidus ) embryos. To conduct chilling studies, we subjected S. embryos incubation at either 26 °C (control) or 5 0, 4, 8, 16, 32 h. The software tools GeNorm, NormFinder, Bestkeeper were employed identify HKG. GeNorm identified 3 rRNA as stable genes. By contrast, NormFinder determined that a eye-formation pre-hatch stage Finally, gapdh β-actin are This first HKGs studying temperatures. Its findings can aid future research evaluating

Language: Английский

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Algal Symbiont Diversity and Host Fitness Variation in Amoebozoan Photosymbiosis

Journal of Eukaryotic Microbiology, Journal Year: 2025, Volume and Issue: 72(3)

Published: April 25, 2025

ABSTRACT Photosymbioses, the symbiotic relationships between microalgae and non‐photosynthetic eukaryotes, are sporadically found in many eukaryotic lineages. Only a few taxa, such as cnidarians ciliates hosting algal endosymbionts, have been actively studied, which has hindered understanding universal mechanisms of photosymbiosis establishment. In Amoebozoa, species reported photosymbiotic, how photosymbioses established is still unclear. To investigate extent to one photosymbiotic amoebae, Mayorella viridis , depends on their symbionts, amoebae were treated with reagents known induce collapsing other species. We succeeded removing symbionts from hosts 2‐amino‐3‐chloro‐1,4‐naphthoquinone. While apo‐symbiotic grew same state when they fed prey, survival rates lower than those ones during starvation, suggesting that impact fitness condition‐dependent. Furthermore, we showed was reversible by feeding two strains green alga Chlorella amoebae. The efficiencies ingesting cells significantly differed strains. These results suggest relationship amoeba facultative different discrete abilities amoeba.

Language: Английский

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Characterization of protein glycosylation in an Asgard archaeon

BBA Advances, Journal Year: 2024, Volume and Issue: 6, P. 100118 - 100118

Published: Jan. 1, 2024

Archaeal cells are typically enveloped by glycosylated S-layer proteins. protein glycosylation provides valuable insights not only into their adaptation to niches but also evolutionary trajectory. Notably, thermophilic Thermoproteota modify proteins with N-glycans that include two GlcNAc units at the reducing end, resembling "core structure" preserved across eukaryotes. Recently, Asgard archaea, now classified as members of phylum Promethearchaeota, have offered unprecedented opportunities for understanding role archaea in eukaryogenesis. Despite presence genes indicative N-glycosylation this archaeal group, these been experimentally investigated. Here we performed a glycoproteome analysis firstly isolated archaeon Promethearchaeum syntrophicum. Over 700 different were identified through high-resolution LC-MS/MS analysis, however, there was no evidence either or putative Instead, primarily observed an extracellular solute-binding protein, possibly related chemoreception transmembrane transport oligopeptides. The glycan modification occurred on asparagine residue located within conserved N-X-S/T sequon, consistent pattern found other bacteria, and Unexpectedly, three structurally lacking conventional core structure archaeon, presenting unique compositions included atypical sugars. one sugars likely HexNAc modified threonine residue, similar modifications previously mesophilic methanogens Methanobacteriati. Our findings advance our physiology dynamics.

Language: Английский

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Trade-off between photosymbiosis and innate immunity influences cnidarian’s response to pathogenic bacteria

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 291(2032)

Published: Oct. 1, 2024

Mutualistic relationships with photosynthetic organisms are common in cnidarians, which form an intracellular symbiosis dinoflagellates the family Symbiodiniaceae. The establishment and maintenance of these symbionts associated suppression key host immune factors. Because this, there potential trade-offs between nutrition that cnidarian hosts gain from their ability to successfully defend themselves pathogens. To investigate trade-offs, we utilized facultatively symbiotic polyps upside-down jellyfish Cassiopea xamachana exposed aposymbiotic pathogen Serratia marcescens . Symbiotic had a lower probability survival following S. exposure. Gene expression analyses 24 hours exposure indicate animals mounted more damaging response, higher levels inflammation oxidative stress likely resulting severe disruptions cellular homeostasis. Underlying this response may be differences constitutive pathogen-induced transcription factors rather than broadscale during symbiosis. Our findings polyps, hosting limits C. xamachana’ s survive exposure, indicating trade-off immunity has implications for coral disease research.

Language: Английский

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Comparative single cell analysis reveals complex patterns of cell type and cell signaling innovations at the fetal-maternal interface

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 2, 2024

How fetal and maternal cell types have co-evolved to enable mammalian placentation poses a unique evolutionary puzzle. Here, we present multi-species atlas integrating single-cell transcriptomes from six species bracketing therian mammal diversity. We find that invasive trophoblasts share gene-expression signature across eutherians, evidence endocrine decidual cells evolved stepwise an immunomodulatory type retained in

Language: Английский

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An Improved RNA Extraction Method for Octocorals and Its Application in Transcriptome Analysis of Dark-Induced Bleaching Octocoral

Marine Biotechnology, Journal Year: 2024, Volume and Issue: 27(1)

Published: Nov. 26, 2024

Language: Английский

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Tracking the early events of photosymbiosis evolution

Trends in Plant Science, Journal Year: 2023, Volume and Issue: 29(4), P. 406 - 412

Published: Nov. 27, 2023

Language: Английский

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Comparative transcriptome analysis reveals deep molecular landscapes in stony coral Montipora clade

Frontiers in Genetics, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 7, 2023

Introduction: Coral reefs, among the most invaluable ecosystems in world, face escalating threats from climate change and anthropogenic activities. To decipher genetic underpinnings of coral adaptation resilience, we undertook comprehensive transcriptome profiling two emblematic species, Montipora foliosa capricornis, leveraging PacBio Iso-Seq technology. These species were strategically selected for their ecological significance taxonomic proximity within Anthozoa class. Methods: Our study encompassed generation pristine transcriptomes, followed by thorough functional annotation via diverse databases. Subsequently, quantified transcript abundance scrutinized gene expression patterns, revealing notable distinctions between species. Results: Intriguingly, shared orthologous genes identified across a spectrum highlighting substantial conservation scleractinian corals. Importantly, subset genes, integral to biomineralization processes, emerged as exclusive corals, shedding light on intricate evolutionary history. Furthermore, discerned pronounced upregulation linked immunity, stress response, oxidative-reduction processes M. relative capricornis. findings hint at presence more robust mechanisms maintaining internal equilibrium effectively navigating external challenges, underpinning its potential advantage. Beyond elucidating our research underscores urgency preserving diversity populations. Discussion: insights hold promise informed strategies aimed safeguarding these imperiled ecosystems, bearing economic significance. In synthesis, seamlessly integrates genomic inquiry with relevance, bridging gap molecular imperative conserve reefs mounting threats.

Language: Английский

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Comparative Transcriptomics of Fat Bodies between Symbiotic and Quasi-Aposymbiotic Adult Females of Blattella germanica with Emphasis on the Metabolic Integration with Its Endosymbiont Blattabacterium and Its Immune System

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(8), P. 4228 - 4228

Published: April 11, 2024

We explored the metabolic integration of

Language: Английский

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Single-cell RNA-sequencing reveals immune system compartmentalization under symbiosis in the stony coralOculina arbuscula

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 24, 2024

ABSTRACT Many cnidarians host single-celled algae within gastrodermal cells, yielding a mutually beneficial exchange of nutrients between and symbiont, dysbiosis can lead to mortality. Previous research has uncovered symbiosis tradeoffs, including suppression the NF-κB immune pathway in hosting intracellular correlations symbiotic state pathogen susceptibility. Here, we used multiomic approach characterize states facultatively coral Oculina arbuscula by generating genotype-controlled fragments aposymbiotic tissue. Metabarcoding 16S rRNA showed no difference bacterial communities states. Consistent with previous whole-organism RNA-seq studies, proteomics revealed differential abundance proteins related immunity, consistent during symbiosis. Moreover, single-cell RNAseq identified diverse cell clusters six types across Specifically, cluster containing algal-hosting cells from tissue had higher expression nitrogen cycling sugar transport genes than Furthermore, lower involved regulation, genes, was observed these However, differences gene were This work reveals compartmentalization system specific symbiosis, which likely limits tradeoffs dampening immunity algal while still maintaining general organismal immunity.

Language: Английский

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