ACS Synthetic Biology,
Journal Year:
2024,
Volume and Issue:
13(11), P. 3746 - 3753
Published: Oct. 31, 2024
Ribosomally
synthesized
lanthionine-containing
peptides
(lanthipeptides)
have
emerged
as
a
promising
source
of
antimicrobials
against
multidrug
resistance
pathogens.
An
effective
way
to
discover
and
engineer
lanthipeptides
is
through
heterologous
expression
their
biosynthetic
gene
clusters
(BGCs)
in
host
choice.
Here
we
report
plug-and-play
pathway
refactoring
strategy
for
rapid
evaluation
lanthipeptide
BGCs
Bacillus
subtilis
based
on
the
T7
system.
As
proof
concept,
used
this
not
only
observe
successful
production
known
haloduracin
β
but
also
two
new
human-microbiota-derived
that
previously
failed
be
produced
Escherichia
coli.
The
resulting
B.
system
should
enable
genome
mining
high-throughput
manner.
Journal of Personalized Medicine,
Journal Year:
2024,
Volume and Issue:
14(2), P. 217 - 217
Published: Feb. 18, 2024
Infectious
diseases
have
long
posed
a
significant
threat
to
global
health
and
require
constant
innovation
in
treatment
approaches.
However,
recent
groundbreaking
research
has
shed
light
on
previously
overlooked
player
the
pathogenesis
of
disease-the
human
microbiome.
This
review
article
addresses
intricate
relationship
between
microbiome
infectious
unravels
its
role
as
crucial
mediator
host-pathogen
interactions.
We
explore
remarkable
potential
harnessing
this
dynamic
ecosystem
develop
innovative
strategies
that
could
revolutionize
management
diseases.
By
exploring
latest
advances
emerging
trends,
aims
provide
new
perspective
combating
by
targeting
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(19), P. 10788 - 10788
Published: Oct. 8, 2024
Antimicrobial
peptides
(AMPs)
are
short,
usually
cationic
with
an
amphiphilic
structure,
which
allows
them
to
easily
bind
and
interact
the
cellular
membranes
of
viruses,
bacteria,
fungi,
other
pathogens.
Bacterial
AMPs,
or
bacteriocins,
can
be
produced
from
Gram-negative
Gram-positive
bacteria
via
ribosomal
synthesis
eliminate
competing
organisms.
AMPs
vital
in
addressing
increasing
antibiotic
resistance
various
pathogens,
potentially
serving
as
alternative
ineffective
antibiotics.
Bacteriocins
have
a
narrow
spectrum
action,
making
highly
specific
antibacterial
compounds
that
target
particular
bacterial
This
review
covers
two
main
groups
bacteriocins
by
their
modes
classification,
sources
positive
effects
they
play
on
human
body,
limitations
future
perspectives
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Abstract
The
biosynthesis
of
ribosomally
synthesized
and
post-translationally
modified
peptides
(RiPPs)
leverages
iterative
catalysis
to
enhance
structural
biological
diversity.
Traditionally,
enzymes
install
multiple
post-translational
modifications
(PTMs)
on
linear
peptides,
rather
than
mature
RiPPs
with
intricate
three-dimensional
structures,
which
would
require
complex
changes
in
substrate
binding
poses.
Here,
we
present
a
prolific
class
GCN5-related
N-acetyltransferases
(GNATs)
that
iteratively
consecutively
acylate
two
Lys
residues
within
the
loop
ring
motifs
lasso
diverging
from
typical
modification
peptides—an
unprecedented
function
for
PTM
enzymes.
Utilizing
high-resolution
cryo-electron
microscopy
enzymatic
reconstitution,
mapped
peptide
pocket
IatT
pinpointed
key
involved
demarcating
distinct
acetylation
steps.
Structure-based
engineering
IatT’s
acetyl
group
recognition
site
expanded
cavity
accommodate
longer-chain
acyl
groups,
leading
creation
lipolasso
novel
ribosomal
lipopeptide.
This
strategy
can
be
applied
any
RiPP
BGC
encoding
GNAT,
facilitating
efficient
diversification
rare
lipopeptides.
Graphic
Chemical Society Reviews,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Cell
free
synthetic
biology
enables
a
broad
range
of
approaches
relevant
to
the
generation,
biosynthetic
characterisation,
bioengineering,
activity
profiling,
and
production
complex
natural
products.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(4), P. 1767 - 1773
Published: Jan. 17, 2024
Lanthipeptides
make
up
a
large
group
of
natural
products
that
belong
to
the
ribosomally
synthesized
and
post-translationally
modified
peptides
(RiPPs).
contain
lanthionine
methyllanthionine
bis-amino
acids
have
varying
stereochemistry.
The
stereochemistry
new
lanthipeptides
is
often
not
determined
because
current
methods
require
equipment
standard
in
most
laboratories.
In
this
study,
we
developed
facile,
efficient,
user-friendly
method
for
detecting
lanthipeptide
stereochemistry,
utilizing
advanced
Marfey's
analysis
with
detection
by
liquid
chromatography
coupled
mass
spectrometry
(LC-MS).
Under
optimized
conditions,
0.05
mg
peptide
sufficient
characterize
five
(methyl)lanthionines
different
using
simple
setup,
which
much
lower
limit
than
methods.
addition,
describe
readily
access
standards
three
stereoisomers
two
been
reported
known
lanthipeptides.
workflow
uses
commonly
used
nonchiral
column
system
offers
scalable
platform
assist
antimicrobial
discovery.
We
illustrate
its
utility
an
example
discovered
genome
mining.
Biomolecules,
Journal Year:
2024,
Volume and Issue:
14(4), P. 479 - 479
Published: April 15, 2024
Ribosomally
synthesized
and
post-translationally
modified
peptides
(RiPPs)
represent
a
significant
potential
for
novel
therapeutic
applications
because
of
their
bioactive
properties,
stability,
specificity.
RiPPs
are
on
ribosomes,
followed
by
intricate
post-translational
modifications
(PTMs),
crucial
diverse
structures
functions.
PTMs,
such
as
cyclization,
methylation,
proteolysis,
play
roles
in
enhancing
RiPP
stability
bioactivity.
Advances
synthetic
biology
bioinformatics
have
significantly
advanced
the
field,
introducing
new
methods
production
engineering.
These
encompass
strategies
heterologous
expression,
genetic
refactoring,
exploiting
substrate
tolerance
tailoring
enzymes
to
create
analogs
with
improved
or
entirely
Furthermore,
introduction
implementation
cutting-edge
screening
methods,
including
mRNA
display,
surface
two-hybrid
systems,
expedited
identification
pharmaceutical
potential.
This
comprehensive
review
not
only
discusses
current
advancements
research
but
also
promising
opportunities
that
leveraging
these
presents,
illustrating
synergy
between
traditional
biochemistry
contemporary
engineering
approaches.
Briefings in Bioinformatics,
Journal Year:
2024,
Volume and Issue:
25(4)
Published: May 23, 2024
Abstract
How
to
resolve
the
metabolic
dark
matter
of
microorganisms
has
long
been
a
challenging
problem
in
discovering
active
molecules.
Diverse
omics
tools
have
developed
guide
discovery
and
characterization
various
microbial
metabolites,
which
make
it
gradually
possible
predict
overall
metabolites
for
individual
strains.
The
combinations
multi-omic
analysis
effectively
compensates
shortcomings
current
studies
that
focus
only
on
single
or
broad
class
metabolites.
In
this
review,
we
systematically
update,
categorize
sort
out
different
prediction
last
five
years
appeal
combination
understanding
nature
microbes.
First,
provide
general
survey
updated
databases,
webservers,
software
based
genomics,
transcriptomics,
proteomics,
metabolomics,
respectively.
Then,
discuss
essentiality
integration
multi-omics
data
strains
communities,
as
well
stressing
other
techniques,
such
systems
biology
methods
data-driven
algorithms.
Finally,
identify
key
challenges
trends
developing
more
comprehensive
diverse
contribute
human
health
disease
treatment.
