Exploring the Frontier: The Human Microbiome’s Role in Rare Childhood Neurological Diseases and Epilepsy DOI Creative Commons
Newell Belnap, Keri Ramsey,

Sophia T. Carvalho

et al.

Brain Sciences, Journal Year: 2024, Volume and Issue: 14(11), P. 1051 - 1051

Published: Oct. 23, 2024

Emerging research into the human microbiome, an intricate ecosystem of microorganisms residing in and on our bodies, reveals that it plays a pivotal role maintaining health, highlighting potential for microbiome-based interventions to prevent, diagnose, treat, manage myriad diseases. The objective this review is highlight importance microbiome studies enhancing understanding rare genetic epilepsy related neurological disorders. Studies suggest gut acting through gut-brain axis, impacts development severity epileptic conditions children. Disruptions microbial composition can affect neurotransmitter systems, inflammatory responses, immune regulation, which are all critical factors pathogenesis epilepsy. This growing body evidence points microbiome-targeted therapies, such as probiotics or dietary modifications, innovative approaches managing By harnessing power we stand develop more effective personalized treatment strategies children affected by disease other

Language: Английский

MicrobiomeStatPlots: Microbiome statistics plotting gallery for meta‐omics and bioinformatics DOI Creative Commons

Defeng Bai,

Chuang Ma,

Jiani Xun

et al.

iMeta, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Abstract The rapid growth of microbiome research has generated an unprecedented amount multi‐omics data, presenting challenges in data analysis and visualization. To address these issues, we present MicrobiomeStatPlots, a comprehensive platform offering streamlined, reproducible tools for This integrates essential bioinformatics workflows with pipelines provides 82 distinct visualization cases interpreting datasets. By incorporating basic tutorials advanced R‐based strategies, MicrobiomeStatPlots enhances accessibility usability researchers. Users can customize plots, contribute to the platform's expansion, access wealth knowledge freely on GitHub ( https://github.com/YongxinLiu/MicrobiomeStatPlot ). Future plans include extending support metabolomics, viromics, metatranscriptomics, along seamless integration into omics workflows. bridges gaps visualization, paving way more efficient, impactful research.

Language: Английский

Citations

3
Metagenomic symphony of the intestinal ecosystem: How the composition affects the mind DOI Creative Commons
Stefanie Malan‐Müller, David Martín‐Hernández, Javier R. Caso

et al.

Brain Behavior and Immunity, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

Citations

5
Analysis of Gut Bacterial and Fungal Microbiota in Children with Autism Spectrum Disorder and Their Non-Autistic Siblings DOI Open Access

Mauricio Retuerto,

Hilmi Al-Shakhshir,

Janet Herrada

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(17), P. 3004 - 3004

Published: Sept. 5, 2024

Autism Spectrum Disorder (ASD) is a multifactorial disorder involving genetic and environmental factors leading to pathophysiologic symptoms comorbidities including neurodevelopmental disorders, anxiety, immune dysregulation, gastrointestinal (GI) abnormalities. Abnormal intestinal permeability has been reported among ASD patients it well established that disturbances in eating patterns may cause gut microbiome imbalance (i.e., dysbiosis). Therefore, studies focusing on the potential relationship between microbiota are emerging. We compared bacteriome mycobiome of cohort subjects with their non-ASD siblings. Differences include significant decrease at phylum level Cyanobacteria (0.015% vs. 0.074%, p < 0.0003), genus Bacteroides (28.3% 36.8%, 0.03). Species-level analysis showed Faecalibacterium prausnitzii, Prevotella copri, fragilis, Akkermansia municiphila. Mycobiome an increase fungal Ascomycota (98.3% 94%, 0.047) Candida albicans (27.1% 13.2%, 0.055). Multivariate organisms from Delftia were predictive increased odds ratio ASD, whereas decreases Azospirillum associated ASD. screened 24 probiotic identify strains could alter growth identified as elevated within subject samples. In preliminary vivo preclinical test, we challenged wild-type Balb/c mice acidovorans (increased subjects) by oral gavage changes behavioral sham-treated controls. An vitro biofilm assay was used determine ability potentially beneficial microorganisms biofilm-forming acidovorans, break down fiber. Downregulation cyanobacteria (generally for inflammation wound healing) combined species such D. suggests ASD-related GI result concomitant pathogens, probiotics can be counteract these changes.

Language: Английский

Citations

4
Droplet microfluidics: unveiling the hidden complexity of the human microbiome DOI

Yibin Xu,

Zhiyi Wang, Caiming Li

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The human microbiome is vital for health. Droplet microfluidics offers a versatile toolbox research, enabling single-cell sequencing, cultivation, and functional analyses to deepen our understanding drive innovations.

Language: Английский

Citations

0
Effect of Probiotics on the Symptomatology of Autism Spectrum Disorder and/or Attention Deficit/Hyperactivity Disorder in Children and Adolescents: Pilot Study DOI Creative Commons
Meritxell Rojo-Marticella, Victoria Arija, Josefa Canals

et al.

Research on Child and Adolescent Psychopathology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 11, 2025

Abstract The aim of this study is to investigate the impact using probiotics with strains related dopamine and gamma-aminobutyric acid production on clinical features autism spectrum disorder (ASD) and/or attention deficit/hyperactivity (ADHD). This randomized, controlled trial involved 38 children ADHD 42 ASD, aged 5–16 years, who received ( Lactiplantibacillus plantarum Levilactobacillus brevis 109/cfu/daily) or placebo for 12 weeks. Parent-reported symptoms were assessed Conners' 3rd-Ed Social Responsiveness Scale Test, 2nd-Ed (SRS-2), completed Conners Continuous Performance (CPT 3) Kiddie CPT, (K-CPT 2). Executive functions, quality life sleep patterns also parent-assessed. Intention-to-treat analyses, controlling sociodemographic nutritional covariates, revealed no significant inter-group differences in parent-reported neuropsychological data after probiotic intervention. However, age-stratified analyses showed improved hyperactivity-impulsivity younger ASD (Cohen’s d = 1.245) 0.692). Intra-group supported these findings aforementioned age intervention group both diagnoses. An improvement impulsivity was observed intra-group analysis CPT commissions scores (probiotic: p 0.001, Cohen’s -1.216; placebo: 0.013, -0.721). A better comfort score (quality life) shown 0.010, 0.722; 0.099, 0.456). used, may improve or/and ASD. Further research warranted explore as an adjunctive therapeutic NDs. Trial registration: clinicaltrials.gov Identifier: NCT05167110.

Language: Английский

Citations

0
Human Untargeted Metabolomics in High-Throughput Gut Microbiome Research: Ethanol vs Methanol DOI Creative Commons
Simone Zuffa, Vincent Charron‐Lamoureux, Caitriona Brennan

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Untargeted metabolomics is frequently performed on human fecal samples in conjunction with sequencing to unravel the gut microbiome functionality. As sample collection efforts are rapidly expanding, individuals often collecting specimens at home, experiments should adapt accommodate safety and needs of bulk off-site collections improve high throughput. Here, we show that a 95% ethanol, safe be shipped handled, extraction part Matrix Method pipeline recovers comparable amounts metabolites as validated 50% methanol extraction, preserving metabolic profile differences between investigated subjects. Additionally, metabolome remains relatively stable when stored ethanol for up 1 week room temperature. Finally, suggest data analysis workflow based robust centered log ratio transformation, which removes variance introduced by possible different weights concentrations, allowing reliable integration-ready untargeted research.

Language: Английский

Citations

0
Cross-talks between osteoporosis and gut microbiome DOI
Shiva Shankar Jha, Naveen Jeyaraman,

Madhan Jeyaraman

et al.

World Journal of Orthopedics, Journal Year: 2025, Volume and Issue: 16(3)

Published: March 12, 2025

Language: Английский

Citations

0
Revealing gut microbiota biomarkers associated with melanoma immunotherapy response and key bacteria-fungi interaction relationships: evidence from metagenomics, machine learning, and SHAP methodology DOI Creative Commons
Yuhang Zhou, Wenjie Han,

Yun Feng

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 18, 2025

The gut microbiota is associated with the response to immunotherapy in cutaneous melanoma (CM). However, fungal biomarkers and bacterial-fungal interactions have yet be determined. Metagenomic sequencing data of stool samples collected before from three independent groups European ancestry CM patients were collected. After characterizing relative abundances bacteria fungi, Linear Discriminant Analysis Effect Size (LEfSe) analysis, Random Forest (RF) model construction, SHapley Additive exPlanations (SHAP) methodology applied identify key responders CM. Diversity analysis revealed significant differences bacterial composition between non-responders. LEfSe identified 45 4 taxa as potential biomarkers. constructing RF model, AUC models built using separately 0.64 0.65, respectively. when combined, merged increased 0.71. In following important biomarkers: Romboutsia, Endomicrobium, Aggregatilinea, Candidatus Moduliflexus, Colwellia, Akkermansia, Mucispirillum, Rutstroemia, which responders, whereas Zancudomyces was Moreover, positive correlation interaction Akkermansia Rutstroemia considered a response. Our results provide valuable insights for enrichment patients. this study highlights critical role immunotherapy.

Language: Английский

Citations

0
Recent advances in therapeutic probiotics: insights from human trials DOI
Mu-Yeol Cho,

Jeong-Sik Eom,

Eun-Mi Choi

et al.

Clinical Microbiology Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

SUMMARY Recent advances in therapeutic probiotics have shown promising results across various health conditions, reflecting a growing understanding of the human microbiome’s role and disease. However, comprehensive reviews integrating diverse effects subjects been limited. By analyzing randomized controlled trials (RCTs) meta-analyses, this review provides overview key developments probiotic interventions targeting gut, liver, skin, vaginal, mental, oral health. Emerging evidence supports efficacy specific strains combinations treating wide range disorders, from gastrointestinal (GI) liver diseases to dermatological bacterial vaginosis, mental diseases. We discuss expanding microbiome-organ connections underlying mechanisms action. While many clinical demonstrate significant benefits, we acknowledge areas requiring further large-scale studies establish definitive optimal treatment protocols. The addresses challenges standardizing research methodologies emphasizes importance considering individual variations microbiome composition host genetics. Additionally, explore emerging concepts such as oral-gut-brain axis future directions, including high-resolution profiling, host-microbe interaction studies, organoid models, artificial intelligence applications research. Overall, offers update on current state multiple domains health, providing insights into directions potential for revolutionize preventive medicine.

Language: Английский

Citations

0
Comprehensive analysis of gut microbiota and fecal metabolites in patients with autism spectrum disorder DOI Creative Commons

Ruijuan Zheng,

Shu Huang,

Pengya Feng

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 25, 2025

Introduction Autism spectrum disorder (ASD) is a neurodevelopmental characterized by deficits in social communication and the presence of restricted, repetitive behaviors or interests. Studies have revealed that gut microbiota their metabolism play important roles ASD, become underlying mechanisms ASD. Methods In this study, we performed long-read 16S rRNA sequencing untargeted metabolomics to comprehensively characterize profiles fecal metabolites 34 ASD patients 18 healthy controls. The associations between microbiota, clinical symptoms were analyzed screen related biomarkers for Results results showed similarity overall microbial richness diversity controls, however, some specific bacterial taxa exhibited significant differences, including Klebsiella Escherichia-Shigella at genera level, Clostridium-sporogenes , Escherichia-coli-O157H7 Bacteroides-ovatus species level. validated lot had significantly differential levels, series organic acids, amino acids dopamine. Discussion might shed new light on pathogenesis help us understand importance as potential therapeutic targets development

Language: Английский

Citations

0