Bacteria-tumor symbiosis destructible novel nanocatalysis drug delivery systems for effective tumor therapy DOI

Jing Zang,

Yin Fang, Ziyuan Liu

et al.

Nanomedicine, Journal Year: 2024, Volume and Issue: 20(3), P. 305 - 318

Published: Dec. 30, 2024

Colorectal cancer (CRC) is a significant threat to human health. The dynamic equilibrium between probiotics and pathogenic bacteria within the gut microbiota crucial in mitigating risk of CRC. An overgrowth harmful microorganisms gastrointestinal tract can result an excessive accumulation bacterial toxins carcinogenic metabolites, thereby disrupting delicate balance microbiota. This disruption may lead alterations microbial composition, impairment mucosal barrier function, potential promotion abnormal cell proliferation, ultimately contribute progression Recently, research has indicated that intestinal presence Fusobacterium nucleatum (Fn) significantly influences onset, progression, metastasis Consequently, interaction CRC cells Fn presents promising strategy against Nanomaterials have been extensively utilized therapy infection control, demonstrating substantial treating bacteria-associated tumors. review begins by elucidating mechanisms occurrence CRC, with particular emphasis on clarifying intricate relationship Subsequently, we highlight strategies utilize nanomaterials disrupt association Overall, this offers valuable insight guidance for leveraging therapy.

Language: Английский

Intra-tumoral bacteria in breast cancer and intervention strategies DOI
Ting Hou, Xiaoling Huang,

Jiahui Lai

et al.

Advanced Drug Delivery Reviews, Journal Year: 2025, Volume and Issue: unknown, P. 115516 - 115516

Published: Jan. 1, 2025

Language: Английский

Citations

2
Virulence factor discovery identifies associations between the Fic gene family and Fap2 + fusobacteria in colorectal cancer microbiomes DOI Creative Commons
Geicho Nakatsu,

Duhyun Ko,

Monia Michaud

et al.

mBio, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Fusobacterium is a bacterium associated with colorectal cancer (CRC) tumorigenesis, progression, and metastasis. Fap2 fusobacteria-specific outer membrane galactose-binding lectin that mediates adherence to invasion of CRC tumors. Advances in omics analyses provide an opportunity profile identify microbial genomic features correlate the cancer-associated bacterial virulence factor Fap2. Here, we analyze genomes colon tumor isolates find family post-translational modification enzymes containing Fic domains positivity these strains. We demonstrate genes expand presence fusobacterial pangenome. Through comparative analysis, Fap2+ Fusobacteriota are highly enriched gene families compared other human gut microbiome taxa. Using global data set shotgun metagenomes, show frequently co-occur fecal microbiomes individuals late-stage CRC. further characterize specific harbored by animalis detect recombination events elements horizontal transfer via synteny analysis loci. Exposure F. strain adenocarcinoma cell line increases expression virulence-associated adhesins. Finally, proteins synthesized as peptides detectable monoculture supernatants. Taken together, our study uncovers potential factors genomes.IMPORTANCEAccumulating support members intra-tumoral microbiota critically influence progression. Yet, relatively little known about non-adhesin may carcinogenesis. Our assays fusobacteria domain-containing genes, well-studied pathogenic bacteria, features. The encoded expressed merit future investigation assess their roles development

Language: Английский

Citations

1
Establishment and improvement of genetic manipulation tools for Fusobacterium nucleatum DOI Creative Commons
Zhiwei Guan, Hailong Wang, Qiang Feng

et al.

Engineering Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 100192 - 100192

Published: Feb. 1, 2025

Language: Английский

Citations

1
The interplay between gut bacteria and targeted therapies: implications for future cancer treatments DOI Creative Commons
Juan He, Yu Chen,

Huakan Zhao

et al.

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: Feb. 13, 2025

Language: Английский

Citations

1
An interplay between human genetics and intratumoral microbiota in the progression of colorectal cancer DOI

Jing Yu,

Yuxuan Liang, Qingrong Zhang

et al.

Cell Host & Microbe, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

1
Development of a conditional plasmid for gene deletion in non-model Fusobacterium nucleatum strains DOI Creative Commons
Peng Zhou,

G C Bibek,

Chenggang Wu

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 24, 2025

ABSTRACT Fusobacterium nucleatum is an opportunistic pathogen with four subspecies: (FNN), vincentii (FNV), polymorphum (FNP), and animalis (FNA), each distinct disease potentials. Research on fusobacterial pathogenesis has mainly focused the model strain ATCC 23726 from FNN. However, this narrow focus may overlook significant behaviors of other FNN strains those subspecies, given genetic phenotypic diversity within F. . While highly transformable, most exhibit low transformation efficiency, complicating traditional gene deletion methods that rely non-replicating plasmids. To address this, we developed a conditional plasmid system in which RepA protein, essential for replication pCWU6-based shuttle plasmid, controlled by inducible combining fdx promoter theophylline-responsive riboswitch. This allows host cells upon induction loss when inducer removed, forcing chromosomal integration via homologous recombination presence antibiotic thiamphenicol. We validated approach targeting galK gene, successfully generating mutants (ATCC 23726, CTI-2), FNP 10953), FNA (21_1A), closely related species periodonticum Incorporating sacB counterselection marker enabled radD non-model strains. Interestingly, while 10953, 21_1A abolished coaggregation Actinomyces oris , CTI-2 mutant retained ability, suggesting involvement unknown adhesins. work significantly advances genetically recalcitrant strains, enhancing our understanding pathogen. IMPORTANCE implicated various human diseases, including periodontal disease, preterm birth, colorectal cancer, often linked to specific reflecting species’ diversity. Despite diversity, research centered potentially missing key aspects strains’ pathogenic potential. study addresses critical gap developing novel enables deleted genes clinical CTI-2, 21_1A, first time. Our findings, particularly varying behavior production across underscore complexity need broader studies. virulence at level provides valuable tool future bacterial genetics research.

Language: Английский

Citations

0
Oral Fusobacterium nucleatum exacerbates ulcerative colitis via the oral-gut axis: mechanisms and therapeutic implications DOI Creative Commons
Zhili Zheng, Wenqin Jin, Weiwei Guo

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 7, 2025

Fusobacterium nucleatum (F. nucleatum) is an anaerobic bacterium known for its association with periodontal disease and oral infections. It has been implicated in the development of gastrointestinal diseases such as inflammatory bowel colorectal cancer. Ulcerative colitis (UC), which characterized by chronic inflammation colon, a condition unknown etiology rising incidence rate, significantly affecting quality life patients. The increased intestinal permeability during UC may facilitate adherence or invasion F. into damaged barrier, leading to exacerbated inflammation. This article introduces concept oral-gut axis, reviewing existing literature analyze role pathogenesis exploring potential pathogenic mechanisms. also summarizes latest advances treating patients who have looks forward prospective therapeutic strategies translational prospects within axis. be key player UC, likely due invasiveness periods permeability. paper discusses innovative approaches prevention management nucleatum, paving way more effective treatment UC. review offers new insights complex relationship between microbiome diseases, enhancing our understanding their dynamic interactions. There paucity on approaches, indicating need further clinical research.

Language: Английский

Citations

0
Unveiling the complex extra‐oral colonization pathways and pathogenic mechanisms of Fusobacterium nucleatum, a heterogeneous oral pathogen DOI Creative Commons
Zhiwei Guan,

Houbao Qi,

Qiang Feng

et al.

View, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Abstract Fusobacterium nucleatum is a heterogeneous bacterium that includes four subspecies. It not only an opportunistic pathogen of periodontitis but also can be frequently detected at the lesion sites various systemic diseases. Extensive research has underscored significant involvement F. in onset, progression, and therapeutic responses numerous oral systematic Given its convenient isolation from lesions, feasibility to cultivate vitro, diverse pathogenic pathways, emerged as “model species” for elucidating effects pathogens on human health. Here, we begin by introducing taxonomic characteristics . Next, explore ’s ability adhere invade host cells, which serves critical prerequisite pathogenicity. Subsequently, analyze pathways through achieves ectopic colonization, revealing how it establishes infections non‐oral such intestine reproductive tract. Furthermore, elaborate mechanisms, particularly cardiovascular diseases, adverse pregnancy outcomes, cancer, focusing modulates immune virulence factors signaling pathways. Finally, summarize advances understanding regulatory mechanisms underlying pathogenesis. We aim provide theoretical foundation developing clinical interventions targeting offer insights directions future investigations into pathogenesis

Language: Английский

Citations

0
Oral microbiota signature predicts the prognosis of colorectal carcinoma DOI Creative Commons
Shuaishuai Zhou, Yan Du,

Wen‐Qiong Xue

et al.

npj Biofilms and Microbiomes, Journal Year: 2025, Volume and Issue: 11(1)

Published: May 5, 2025

Emerging evidence links oral-derived gut microbes to colorectal cancer (CRC) development, but CRC prognosis-related microbial alterations in oral remain underexplored. In a retrospective study of 312 patients, we examined the microbiota using 16S rRNA gene full-length amplicon sequencing identify prognostic biomarkers for CRC. Neisseria oralis and Campylobacter gracilis increased progression risk (HR = 2.63 with P 0.007, HR 2.27 0.001, respectively), while Treponema medium showed protective effects 0.41, 0.0002). A score (MRS) incorporating these species effectively predicted (C-index 0.68, 95% CI 0.61-0.76). When compared model constructed solely from clinical factors, including tumor stage, lymphatic metastasis, perineural invasion, predictive accuracy significantly improved addition MRS, resulting C-index rising 0.77 (P 2.33 × 10-5). Our findings suggest that may contribute personalized monitoring strategies, their implementation surveillance necessitates confirmatory studies.

Language: Английский

Citations

0
Microbiota mechanisms in cancer progression and therapy DOI
Xing Zhang, Kyong Tkhe Fam, Tingting Dai

et al.

Cell chemical biology, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0