Asgard Arf GTPases can act as membrane-associating molecular switches with the potential to function in organelle biogenesis DOI Creative Commons
Jing Zhu, Ruize Xie, Qingguang Ren

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 17, 2025

Inward membrane budding, i.e., the bending of towards cytosol, is essential for forming and maintaining eukaryotic organelles. In eukaryotes, Arf GTPases initiate this inward budding. Our research shows that Asgard archaea genomes encode putative proteins (AArfs). AArfs possess structural elements characteristic their counterparts. When expressed in yeast mammalian cells, some displayed GTP-dependent targeting. vitro, AArf associated with both archaeal membranes. yeast, interacted were regulated by key organelle biogenesis players. Expressing an led to a massive proliferation endomembrane organelles including endoplasmic reticulum Golgi. This Sec23, COPII vesicle coat component, manner. These findings suggest certain are membrane-associating molecular switches functional potential biogenesis, evolution could be next critical step establishing cell architecture. The formation was pivotal point evolution. Here they show Archaea Arf-like can perform organelle-producing mechanisms when cell, laying foundation

Language: Английский

Mosaic evolution of eukaryotic carbon metabolism DOI
John M. Archibald

Nature Ecology & Evolution, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Language: Английский

Citations

0
Asgard Arf GTPases can act as membrane-associating molecular switches with the potential to function in organelle biogenesis DOI Creative Commons
Jing Zhu, Ruize Xie, Qingguang Ren

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 17, 2025

Inward membrane budding, i.e., the bending of towards cytosol, is essential for forming and maintaining eukaryotic organelles. In eukaryotes, Arf GTPases initiate this inward budding. Our research shows that Asgard archaea genomes encode putative proteins (AArfs). AArfs possess structural elements characteristic their counterparts. When expressed in yeast mammalian cells, some displayed GTP-dependent targeting. vitro, AArf associated with both archaeal membranes. yeast, interacted were regulated by key organelle biogenesis players. Expressing an led to a massive proliferation endomembrane organelles including endoplasmic reticulum Golgi. This Sec23, COPII vesicle coat component, manner. These findings suggest certain are membrane-associating molecular switches functional potential biogenesis, evolution could be next critical step establishing cell architecture. The formation was pivotal point evolution. Here they show Archaea Arf-like can perform organelle-producing mechanisms when cell, laying foundation

Language: Английский

Citations

0