Applied Physics Reviews,
Journal Year:
2025,
Volume and Issue:
12(1)
Published: Feb. 3, 2025
Extracellular
vesicles
(EVs)
have
emerged
as
promising
cancer
biomarkers
due
to
their
encapsulation
of
molecular
signals
reflective
originating
tumor
cells.
Conventional
analytical
methods
often
fall
short
in
comprehensive
EV
profiling,
necessitating
innovative
approaches
for
enhanced
sensitivity
and
selectivity.
This
review
focuses
on
the
utilization
nanoplasmonic
structures
optical
signal
detection
EVs,
exploring
advancements,
challenges,
future
prospects
toward
single
profiling.
Nanoplasmonic
offer
readout
capabilities,
leveraging
light
iridescence,
plasmonic
amplification
suitable
size
range
complexity
EVs.
We
delve
into
research
implications
on-chip
methods,
shedding
EVs'
role
health
disease.
Despite
notable
progress,
opportunities
still
exist
further
develop
arrays,
customizing
them
bioanalytes
interest,
crucial
both
label-free
labeled
techniques
attain
objectives
One
such
example
is
use
specific
antibodies
surface
functionalization
arrays.
Other
involve
tailoring
design
platforms
physical
properties
target
thereby
enhancing
characterization
capabilities.
The
subsequent
sections
will
cover
a
curated
selection
relevant
studies.
later
discuss
analysis
through
nanoarrays
clinical
sample
scenarios.
While
patterning
colloidal
self-assembly
e-beam
lithography,
enable
integration
with
microfluidic
systems,
facilitating
investigations,
few
technologies
entered
trials.
roadblock
highlights
need
development
cost-effective,
detailed
profiling
methods.
Moreover,
we
avenues
like
machine
learning
address
challenges
related
heterogeneity
EVs
liquid
biopsy
biomarkers.
Finally,
developing
nanoplasmonic-assisted
studied
driving
advancements
diagnostic
therapeutic
realms,
customizable
coupled
artificial
intelligence
modules,
path
forward
precise
which
may
personalized
interventions.
Chemical Society Reviews,
Journal Year:
2023,
Volume and Issue:
52(24), P. 8500 - 8530
Published: Jan. 1, 2023
This
review
explores
various
point-of-care
optical
diagnostic
systems
combined
with
microdevices
developed
during
the
recent
COVID-19
pandemic
for
clinical
diagnostics.
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(4), P. 2872 - 2884
Published: Jan. 18, 2024
Strategies
for
rapid,
effective
nucleic
acid
processing
hold
tremendous
significance
to
the
clinical
analysis
of
circulating
tumor
DNA
(ctDNA),
a
family
important
markers
indicating
tumorigenesis
and
metastasis.
However,
traditional
techniques
remain
challenging
achieve
efficient
enrichment,
further
bringing
about
complicated
operation
limited
detection
sensitivity.
Here,
we
developed
an
ion
concentration
polarization
microplatform
that
enabled
highly
enrichment
purification
ctDNA
from
variety
samples,
including
serum,
urine,
feces.
The
platform
demonstrated
efficiently
separating
enriching
within
30
s,
with
100-fold
improvement
over
methods.
Integrating
on-chip
isothermal
amplification
module,
achieved
enhanced
sensitivity
in
detection,
which
significantly
eliminated
false-negative
results
serum
or
urine
samples
due
low
abundance
ctDNA.
Such
simple-designed
offers
user-friendly
yet
powerful
diagnosis
technique
wide
applicability,
ranging
early
infection
screening.
ACS Photonics,
Journal Year:
2024,
Volume and Issue:
11(3), P. 904 - 916
Published: Feb. 23, 2024
Metasurfaces
have
revolutionized
optical
biosensing
and
diagnostic
assays
due
to
their
sensitivity,
compactness,
label-free
operation.
However,
metasurface
applications
in
analyzing
complex
biological
systems
quantum
bioscience
phenomena
remain
scarce.
In
this
Perspective,
we
discuss
current
developments
biosensors
propose
promising
future
for
probing
or
adopting
effects
improving
spatial
omics
analysis
live
cells
tissues.
We
the
capabilities
of
platforms
monitoring
relevant
biomarkers
viral
diseases,
neurodegenerative
cancers.
Metasurface-empowered
examination
cell
morphology
secretome,
virus
detection,
tissue
imaging
can
improve
accuracy
early
diagnosis.
Furthermore,
review
device-integration
approaches
point-of-care
testing
settings
that
could
constitute
pathways
toward
technology
commercialization.
Altogether,
provide
a
perspective
exploring
precision
health
medicine.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(45), P. 18011 - 18019
Published: Sept. 27, 2024
Generally,
rolling
circle
amplification
(RCA)
is
based
on
an
enzyme-linked
padlock
extension
reaction.
Herein,
rapid
linking
that
utilizes
click
chemistry
for
joining
sticky
ends
of
DNA
molecules
was
developed.
The
nucleic
acid
were
modified
with
2-cyano-6-aminobenzothiazole
(CBT)
and
cystine
(Cys-Cys),
while
glutathione
introduced
to
break
the
disulfide
bond
under
target
navigation
promote
linkage
between
CBT
Cys
at
terminus
pH
7.4.
Subsequently,
RCA
performed
using
phi29
polymerase.
CRISPR/Cas12a
cleavage
triggered
by
product
amplification.
Assisted
alkaline
phosphatase,
electron
exchange
process
photoelectroactive
Sb@Co(OH)F
nanorod
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
36(15)
Published: Jan. 30, 2024
On-site
diagnostic
tests
that
accurately
identify
disease
biomarkers
lay
the
foundation
for
self-healthcare
applications.
However,
these
routinely
rely
on
single-mode
signals
and
suffer
from
insufficient
accuracy,
especially
multiplexed
point-of-care
(POCTs)
within
a
few
minutes.
Here,
this
work
develops
dual-mode
multiclassification
platform
integrates
an
electrochemiluminescence
sensor
field-effect
transistor
in
microfluidic
chip.
The
channel
guides
testing
samples
to
flow
across
electro-optical
units,
which
produce
readouts
by
detecting
infectious
of
tuberculosis
(TB),
human
rhinovirus
(HRV),
group
B
streptococcus
(GBS).
Then,
machine-learning
classifiers
generate
three-dimensional
(3D)
hyperplanes
diagnose
different
diseases.
Dual-mode
derived
distinct
mechanisms
enhance
anti-interference
ability
physically,
machine-learning-aided
diagnosis
high-dimensional
space
reduces
occasional
inaccuracy
mathematically.
Clinical
validation
studies
with
501
unprocessed
indicate
has
accuracy
approaching
99%,
higher
than
77%-93%
rapid
technologies
at
100%
statistical
power
(>150
clinical
tests).
Moreover,
time
is
5
min
without
trade-off
accuracy.
This
solves
issue
on-site
diagnosis,
endowing
POCT
systems
same
as
laboratory
holding
unique
prospects
complicated
scenes
personalized
healthcare.
