JACS Au,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 27, 2024
DNA
nanostructures
present
new
opportunities
as
Nanotags
for
electron
microscopy
(EM)
imaging,
leveraging
their
high
programmability,
unique
shapes,
biomolecule
conjugation
capability,
and
stability
compatible
with
standard
cryogenic
sample
preparation
protocols.
This
perspective
highlights
the
potential
of
to
enable
high-throughput
multiplexed
EM
analysis
facilitate
in
situ
particle
identification
tomography
(cryo-ET).
Meanwhile,
applying
live-cell
environments
requires
efficient
cellular
uptake
intact
structures
successful
cytosolic
migration.
Promising
strategies
such
employing
direct
delivery
platforms
expressing
RNA-based
are
discussed,
while
more
systematic
studies
needed
fully
understand
intracellular
trafficking
achieve
precise
localization
Nanotags.
Nature Biotechnology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 14, 2025
Efficient
and
accurate
nanocarrier
development
for
targeted
drug
delivery
is
hindered
by
a
lack
of
methods
to
analyze
its
cell-level
biodistribution
across
whole
organisms.
Here
we
present
Single
Cell
Precision
Nanocarrier
Identification
(SCP-Nano),
an
integrated
experimental
deep
learning
pipeline
comprehensively
quantify
the
targeting
nanocarriers
throughout
mouse
body
at
single-cell
resolution.
SCP-Nano
reveals
tissue
distribution
patterns
lipid
nanoparticles
(LNPs)
after
different
injection
routes
doses
as
low
0.0005
mg
kg-1-far
below
detection
limits
conventional
imaging
techniques.
We
demonstrate
that
intramuscularly
injected
LNPs
carrying
SARS-CoV-2
spike
mRNA
reach
heart
tissue,
leading
proteome
changes,
suggesting
immune
activation
blood
vessel
damage.
generalizes
various
types
nanocarriers,
including
liposomes,
polyplexes,
DNA
origami
adeno-associated
viruses
(AAVs),
revealing
AAV2
variant
transduces
adipocytes
body.
enables
comprehensive
three-dimensional
mapping
bodies
with
high
sensitivity
should
accelerate
precise
safe
nanocarrier-based
therapeutics.
Nature Communications,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: Feb. 3, 2025
RNA
degradation
using
ribonuclease
targeting
chimeras
(RiboTACs)
is
a
promising
approach
for
cancer
therapy.
However,
potential
off-target
serious
issue.
Here,
RiboTAC
designed
tumor
microenvironment
triggered
activation.
The
activated
(TaRiboTAC)
incorporates
two
pre-miR-21
binders,
near-infrared
fluorophore
IR780,
an
RGD
peptide
and
phenylboronic
acid
caged
recruiter.
recruiter
embedded
in
the
molecule
exposed
acidic
pH,
cage
removed
by
H2O2
making
TaRiboTAC
responsive
to
high
levels
microenvironment.
It
shown
targets
tissue
degrades
pre-miR-21.
of
TaRiboTACs
significantly
increases
radiotherapeutic
susceptibility
cells
achieving
efficient
suppression
human
lung
adenocarcinoma
A549
tumors
living
mice.
Small Methods,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 2, 2025
Abstract
In
recent
decades,
the
advancement
of
DNA
nanotechnology
enables
precise
nanoscale
organization
diverse
functional
materials
with
templates.
Particularly,
a
variety
DNA‐templated
protein
patterns
are
constructed
as
powerful
tools
for
programming
biomimetic
complexes.
this
review,
progress
in
patterning,
including
cutting‐edge
methods
arranging
proteins
templates,
and
across
varying
dimensions
briefly
summarized.
Representative
applications
biological
analysis
biomedicine
discussed.
DNA‐protein
programmable
dynamics,
which
hold
promise
precision
diagnosis
therapeutics
highlighted.
Finally,
current
challenges
opportunities
fabrication
application
pattering
Nucleic
acids,
including
DNA
and
RNA,
have
been
used
extensively
as
building
blocks
to
construct
sophisticated
nanostructures
through
complementary
base
pairing
with
predetermined
shapes
sizes.
With
remarkable
biocompatibility,
spatial
addressability,
structural
programmability,
self-assembled
nucleic
acid
biomaterials
found
widespread
applications
in
various
biomedical
researches,
drug
delivery,
bioimaging,
or
disease
diagnosis.
Notably,
one
of
the
representative
nanostructures,
origami
has
drawn
much
attention.
In
this
review,
we
summarize
latest
developments
DNA/RNA
design
based
on
single-stranded
(ssDNA),
double-stranded
(dsDNA),
RNA
(ssRNA)
scaffolds
for
a
range
applications,
gene
regulation,
immunomodulation,
receptor
recognition.
Additionally,
challenges
future
opportunities
will
be
discussed.
Advanced Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 13, 2025
Micro/Nanorobots(MNRs)integrated
with
phototherapy
represent
an
emerging
approach
to
cancer
treatment
and
hold
significant
potential
for
addressing
bacterial
infections,
neurological
disorders,
cardiovascular
diseases,
related
conditions.
By
leveraging
micro/nanoscale
motor
systems
in
conjunction
phototherapy,
these
robots
enable
real-time
guidance
monitoring
of
therapeutic
processes,
improving
drug
delivery
precision
efficiency.
This
integration
not
only
enhances
the
effectiveness
but
also
minimizes
damage
surrounding
healthy
tissues.
Nevertheless,
clinical
translation
MNRs-assisted
still
faces
numerous
challenges.
In
this
review,
recent
key
developments
field
are
comprehensively
summarized,
critical
roles
applications
highlighted,
insights
into
future
directions
pathway
toward
large-scale
implementation
provided.
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 21, 2025
Bionano
robots
have
been
recognized
as
a
tumor-selective
and
effective
platform
for
therapeutic
outcomes
they
synergize
the
merits
of
living
organisms
nanoparticles.
Here,
we
construct
self-mineralized
system
(denoted
SO@FeS)
by
employing
facultative
anaerobic
bacterium
Shewanella
oneidensis
MR-1
to
biosynthesize
FeS
NPs
cancer
therapy
with
dual
cell
death
pathways.
Biogenic
are
embedded
into
surface
inherent
photothermal
conversion
ability
low
crystallinity
tend
simultaneously
release
Fe2+
hydrogen
sulfide
(H2S)
in
an
acidic
environment.
As
result,
obtained
SO@FeS
hybrid
can
couple
versatility
nanoparticles
respiration
tumor-targeting
capacities
bacterium,
ultimately
leading
collaborative
clearance
tumor
cells.
Specifically,
cryo-soft
X-ray
tomography
(cryo-SXT)
is
near-native
3D
imaging
modality
that
directly
displays
trafficking
pathway
More
importantly,
cryo-SXT
captures
maps
SO@FeS-initiated
ferroptosis
apoptosis,
evidenced
remodeling
cytoplasmic
organelles.
This
work
offers
valuable
theoretical
insights
from
perspective
organelle
morphology,
links
subcellular
reorganization
pathways,
facilitates
design
nanoplatforms
integrate
multiple
therapies.
ACS Applied Bio Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 2, 2025
Patterned
assembly
of
multivalent
antibody
complexes
using
DNA
nanostructure
templates
holds
the
potential
for
advancing
studies
cellular
signaling
and
smart
theranostic
applications.
However,
evaluating
heterogeneity
in
protein
conjugation
efficiency
at
distinct
sites
on
remains
challenging.
Here,
we
utilize
atomic
force
microscopy
to
measure
coupling
antibodies
various
positions
two-dimensional
rectangular
origami
frameworks
single-molecule
level,
generating
spatial
maps
binding
efficiencies
across
structures.
We
observe
that
a
discrete
distribution
docking
(spacing
least
18
nm)
framework
leads
progressive
decrease
from
periphery
toward
center.
In
contrast,
continuous
∼10
results
higher
center
relative
periphery.
reason
two
opposing
trends
result
trade-offs
among
Coulombic
repulsion,
steric
hindrance,
cooperative
effects.
This
study
presents
quantitative
evaluation
tool
protein-DNA
conjugates,
providing
insights
into
optimizing
framework-based
systems
improved
precision
diagnostics
therapeutic
