Accelerating the implementation of biocatalysis in industry DOI
John M. Woodley

Applied Microbiology and Biotechnology, Journal Year: 2019, Volume and Issue: 103(12), P. 4733 - 4739

Published: May 2, 2019

Language: Английский

Encapsulating and stabilizing enzymes using hydrogen-bonded organic frameworks DOI
Guosheng Chen, Siming Huang, Xiaomin Ma

et al.

Nature Protocols, Journal Year: 2023, Volume and Issue: 18(7), P. 2032 - 2050

Published: May 17, 2023

Language: Английский

Citations

59
Enzyme immobilization on covalent organic framework supports DOI
Qianqian Zhu,

Yunlong Zheng,

Zhenjie Zhang

et al.

Nature Protocols, Journal Year: 2023, Volume and Issue: 18(10), P. 3080 - 3125

Published: Sept. 6, 2023

Language: Английский

Citations

46
Towards the sustainable production of biomass-derived materials with smart functionality: A tutorial review DOI
Ruibin Wang,

Youguang Feng,

Dongqi Li

et al.

Green Chemistry, Journal Year: 2024, Volume and Issue: 26(16), P. 9075 - 9103

Published: Jan. 1, 2024

Towards the sustainable production of biomass-derived materials with smart functionality.

Language: Английский

Citations

24
Directed Evolution and Unusual Protonation Mechanism of Pyridoxal Radical C–C Coupling Enzymes for the Enantiodivergent Photobiocatalytic Synthesis of Noncanonical Amino Acids DOI
Lei Cheng,

Zhiyu Bo,

Benjamin Krohn-Hansen

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Visible light-driven pyridoxal radical biocatalysis has emerged as a new strategy for the stereoselective synthesis of valuable noncanonical amino acids in protecting-group-free fashion. In our previously developed dehydroxylative C–C coupling using engineered PLP-dependent tryptophan synthases, an enzyme-controlled unusual α-stereochemistry reversal and pH-controlled enantiopreference were observed. Herein, through high-throughput photobiocatalysis, we evolved set stereochemically complementary PLP enzymes, allowing both l- d-amino with enhanced enantiocontrol across broad pH window. These newly acid synthases permitted use range organoboron substrates, including boronates, trifluoroborates, boronic acids, excellent efficiency. Mechanistic studies unveiled unexpected racemase activity earlier enzyme variants. This promiscuous was abolished shedding light on origin enantiocontrol. Further mechanistic investigations suggest switch proton donor to account stereoinvertive formation highlighting stereoinversion mechanism that is rare conventional two-electron enzymology.

Language: Английский

Citations

2
Accelerating the implementation of biocatalysis in industry DOI
John M. Woodley

Applied Microbiology and Biotechnology, Journal Year: 2019, Volume and Issue: 103(12), P. 4733 - 4739

Published: May 2, 2019

Language: Английский

Citations

140