Complete bio-degradation of poly(butylene adipate-co-terephthalate) via engineered cutinases

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: March 24, 2023

Abstract Poly(butylene adipate-co-terephthalate) (PBAT), a polyester made of terephthalic acid (TPA), 1,4-butanediol, and adipic acid, is extensively utilized in plastic production has accumulated globally as environmental waste. Biodegradation an attractive strategy to manage PBAT, but effective PBAT-degrading enzyme required. Here, we demonstrate that cutinases are highly potent enzymes can completely decompose PBAT films 48 h. We further show the engineered cutinases, by applying double mutation render more flexible substrate-binding pocket exhibit higher decomposition rates. Notably, these variants produce TPA major end-product, which beneficial feature for future recycling economy. The crystal structures wild type cutinase from Thermobifida fusca complex with substrate analogue also solved, elucidating their modes. These structural biochemical analyses enable us propose mechanism cutinase-mediated degradation.

Language: Английский

---

Critical review of phytoremediation for the removal of antibiotics and antibiotic resistance genes in wastewater

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 870, P. 161876 - 161876

Published: Jan. 27, 2023

Language: Английский

---

Biodegradation of Polymers: Stages, Measurement, Standards and Prospects

Macromol—A Journal of Macromolecular Research, Journal Year: 2023, Volume and Issue: 3(2), P. 371 - 399

Published: June 6, 2023

Nowadays, sustainable and biodegradable bioplastics are gaining significant attention due to resource depletion plastic pollution. An increasing number of environmentally friendly plastics being introduced the market with aim addressing these concerns. However, many final products still contain additives or mix non-biodegradable polymers ensure minimum performance, which often undermines their ecological footprint. Moreover, there is a lack knowledge about all stages biodegradation accuracy in classifying as biodegradable. Therefore, this review provides an overview polymers, elucidating steps mechanisms polymer biodegradation. We also caution readers growing marketing practice “greenwashing” where companies organizations adopt green strategies label more environmental benefits than they have. Furthermore, we present main standards for evaluating biodegradation, tools, tests capable measuring process. Finally, suggest perspectives involving concepts recycling circularity make them sustainable. After all, “throwing away” should not be option because no outside when only one planet.

Language: Английский

---

Substrate-Binding Mode of a Thermophilic PET Hydrolase and Engineering the Enzyme to Enhance the Hydrolytic Efficacy

ACS Catalysis, Journal Year: 2022, Volume and Issue: 12(5), P. 3033 - 3040

Published: Feb. 18, 2022

Polyethylene terephthalate (PET) is among the most extensively produced plastics, but huge amounts of PET wastes that have accumulated in environment become a serious threat to ecosystem. Applying hydrolytic enzymes depolymerize an attractive measure manage pollution, and searching for more effective prerequisite achieve this goal. A thermostable cutinase originates from leaf-branch compost termed ICCG hydrolase reported so far. Here, we illustrated crystal structure complex with analogue, mono(2-hydroxyethyl)terephthalic acid, reveal enzyme–substrate interaction network. Furthermore, applied structure-based engineering modify screened variants exhibit higher efficacy than parental enzyme. As result, several measured melting temperature approaching 99 °C elevated activity were obtained. Finally, crystallographic analyses performed structural stabilization effects mediated by introduced mutations. These results are importance context understanding mechanism action enzyme shall be beneficial development biodegradation platforms.

Language: Английский

---

Conversion of Plastic Waste to Carbon-Based Compounds and Application in Energy Storage Devices

ACS Omega, Journal Year: 2022, Volume and Issue: 7(16), P. 13403 - 13435

Published: April 13, 2022

At present, plastic waste accumulation has been observed as one of the most alarming environmental challenges, affecting all forms life, economy, and natural ecosystems, worldwide. The overproduction materials is mainly due to human population explosion well extraordinary proliferation in global economy accompanied by productivity. Under this threat, development benign green alternative solutions instead traditional disposal methods such conversion into cherished carbonaceous nanomaterials carbon nanotubes (CNTs), quantum dots (CQDs), graphene, activated carbon, porous utmost importance. This critical review thoroughly summarizes different types daily used plastics, their types, properties, ways effect on environment health, treatment materials, carbon-based compounds through synthetic schemes, utilization energy storage devices particularly supercapacitors, future perspectives. main purpose help targeted audience design futuristic study desired field providing information about work done past few years.

Language: Английский

---