Perforin‐Mimicking Molecular Drillings Enable Macroporous Hollow Lignin Spheres for Performance‐Configurable Materials DOI
Hairong Wang, S. C. Tan, Zhenhua Su

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(15)

Published: Jan. 11, 2024

Abstract Despite the first observations that perforin can punch holes in target cells for live/dead cycles human immune system over 110 years ago, emulating this behavior materials science remains challenging. Here, a perforin‐mimicking molecular drilling strategy is employed to engineer macroporous hollow lignin spheres as performance‐configurable catalysts, adhesives, and gels. Using toolbox of 20 compounds, local curvature amphiphilic modulated generate with hole sizes ranging from 0 100 nm. Multiscale control precisely achieved through noncovalent assembly directing catalysis, synthesis, polymerization. Exceptional performance mutations correlate changes size, including an increase catalytic efficiency 50% 100%, transition nonstick synthetics ultrastrong adhesives (adhesion ≈18.3 MPa, exceeding classic epoxies), transformation viscous sols tough nanogels. Thus, study provides robust versatile route mimicking perforin‐induced structural variations cells, representing significant stride toward exquisite orchestration assemblies multiple length scales.

Language: Английский

Protease-Triggered, Spatially Controlled DNA Assembly in Apoptotic Cells for Early Evaluation of Therapeutic Efficacy DOI
Zhenghan Di, Xiulin Yi, Lele Li

et al.

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(14), P. 7931 - 7940

Published: March 29, 2023

Despite numerous advances in the use of DNA as building blocks to assemble complex structures, dearth strategies that allow for protease-controlled situ assembly living cells remains a bottleneck this field. Here, we present modular engineering approach achieve protease-triggered self-assembly apoptotic early evaluation tumor response drug treatment. In design, peptide nucleic acid is introduced bridge engineer with peptides and thus suppress their activity, while caspase-3 (Casp-3) protease-mediated enzymatic cleavage substrate enables activation assembly, generating fluorescence signal output real-time monitoring Casp-3 activity. Furthermore, specific protease triggering imparts spatial selectivity vivo, allowing therapeutic efficacy. Moreover, strategy extended probe activity MMP-2 lymph node metastasis imaging, demonstrating universality approach. This work highlights ways are simple versatile, potential expand repertoire nanotechnology diverse biomedical applications.

Language: Английский

Citations

24
In Situ Self‐Assembled J‐Aggregate Nanofibers of Glycosylated Aza‐BODIPY for Synergetic Cell Membrane Disruption and Type I Photodynamic Therapy DOI
Yichen Liu, Guang‐jian Liu, Wei Zhou

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(40)

Published: Aug. 16, 2023

The in situ self-assembly of exogenous molecules is a powerful strategy for manipulating cellular behavior. However, the direct photochemically inert constituents into supramolecular nano-photosensitizers (PSs) within cancer cells precise photodynamic therapy (PDT) remains challenge. Herein, we developed glycosylated Aza-BODIPY compound (LMBP) capable self-assembling J-aggregate nanofibers cell membrane destruction and type I PDT. LMBP selectively entered human hepatocellular carcinoma HepG2 subsequently self-assembled intracellular nanovesicles through interactions. Detailed studies revealed that these nanostructures generated superoxide radicals (O2- ⋅) exclusively photoinduced electron transfer, thus enabling effective Furthermore, exhibited an aggregation-induced retention effect, which resulted selective toxicity to by disrupting their membranes synergizing with PDT tumor suppression efficacy vivo.

Language: Английский

Citations

24
Recent strategies to develop pH-sensitive injectable hydrogels DOI
Thavasyappan Thambi, Jae Min Jung,

Doo Sung Lee

et al.

Biomaterials Science, Journal Year: 2023, Volume and Issue: 11(6), P. 1948 - 1961

Published: Jan. 1, 2023

"Smart" biomaterials that are responsive to pathological abnormalities an appealing class of therapeutic platforms for the development personalized medications. The such requires novel techniques could precisely deliver agents diseased tissues, resulting in enhanced effects without harming normal tissues. Among various platforms, injectable pH-responsive promising respond change environmental pH. Aqueous solutions exhibit a phase transition from sol-to-gel response pH changes. hydrogel depot can provide spatially and temporally controlled release bioactive including chemotherapeutic drugs, peptides, proteins. Therapeutic imbibed into hydrogels by simple mixing use toxic solvents used long-term storage or situ injection using syringe catheter form stable gel acts as minimally invasive manner. Tunable physicochemical properties hydrogels, biodegradability, ability interact with drugs mechanical properties, control agent. This review highlights advances design biodegradable forming physiological conditions. Special attention has been paid amphoteric their utilization biomedical applications. We also highlight key challenges future directions clinical translation.

Language: Английский

Citations

23
Three‐in‐One Peptide Prodrug with Targeting, Assembly and Release Properties for Overcoming Bacterium‐Induced Drug Resistance and Potentiating Anti‐Cancer Immune Response DOI
Ge Gao, Yao‐Wen Jiang, Jiaxuan Chen

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(23)

Published: March 6, 2024

The presence of bacteria in tumor results chemotherapeutic drug resistance and weakens the immune response colorectal cancer. To overcome bacterium-induced potentiate antitumor immunity, herein a novel molecule Biotin-Lys(SA-Cip-OH)-Lys(SA-CPT)-Phe-Phe-Nap (Biotin-Cip-CPT-Nap) is rationally designed containing four functional motifs (i.e., biotin motif for targeting, Phe-Phe(-Nap) self-assembly, ciprofloxacin derivative (Cip-OH) antibacterial effect, camptothecin (CPT) chemotherapy). Using molecule, strategy intracellular enzymatic nanofiber formation synergistic antibacterium-enhanced chemotherapy immunotherapy achieved. Under endocytosis mediated by highly expressed receptor cancer cell membrane catalysis carboxylesterase cytoplasm, this can be transformed into Biotin-Nap, which self-assembled nanofibers. Meanwhile, antibiotic Cip-OH CPT are released, overcoming enhancing therapeutic efficacy towards This work offers feasible design multifunctional prodrugs to improve efficiency treatment.

Language: Английский

Citations

15
Perforin‐Mimicking Molecular Drillings Enable Macroporous Hollow Lignin Spheres for Performance‐Configurable Materials DOI
Hairong Wang, S. C. Tan, Zhenhua Su

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(15)

Published: Jan. 11, 2024

Abstract Despite the first observations that perforin can punch holes in target cells for live/dead cycles human immune system over 110 years ago, emulating this behavior materials science remains challenging. Here, a perforin‐mimicking molecular drilling strategy is employed to engineer macroporous hollow lignin spheres as performance‐configurable catalysts, adhesives, and gels. Using toolbox of 20 compounds, local curvature amphiphilic modulated generate with hole sizes ranging from 0 100 nm. Multiscale control precisely achieved through noncovalent assembly directing catalysis, synthesis, polymerization. Exceptional performance mutations correlate changes size, including an increase catalytic efficiency 50% 100%, transition nonstick synthetics ultrastrong adhesives (adhesion ≈18.3 MPa, exceeding classic epoxies), transformation viscous sols tough nanogels. Thus, study provides robust versatile route mimicking perforin‐induced structural variations cells, representing significant stride toward exquisite orchestration assemblies multiple length scales.

Language: Английский

Citations

14