Causal genes identification of giant cell arteritis in CD4+ Memory t cells: an integration of multi-omics and expression quantitative trait locus analysis DOI Creative Commons

Qiyi Yu,

Yifan Wu, Xianda Ma

et al.

Inflammation Research, Journal Year: 2025, Volume and Issue: 74(1)

Published: Jan. 7, 2025

Abstract Background Giant cell arteritis (GCA) is a prevalent artery and strongly correlated with age. The role of CD4+ Memory T cells in giant has not been elucidated. Method Through single-cell analysis, we focused on the arteritis. eQTL analysis mendelian randomization identified significant genes which have causal effect risk. were subsequently divided into gene-positive gene-negative groups, then further was conducted. Mendelian plasma proteins, blood-urine biomarkers metabolites also performed. Eventually, PMA induced Jurkat lines used for biological experiments to explore specific functions cells. Results Similarity GCA old samples explored. DDIT4 ARHGAP15 as risk via randomization. ± or indicated differences aspects involving intercellular communication, functional pathways, protein activity, metabolism drug sensitivity between positive negative groups. In vitro experiments, including overexpression knockdown, demonstrated that leading chronic, low-intensity inflammatory state cells, eventually promoting development GCA. Conclusion effects Specifically, exhibit pro-inflammatory promotes cell.

Language: Английский

EULAR recommendations for the use of imaging in large vessel vasculitis in clinical practice: 2023 update DOI Open Access
Christian Dejaco, Sofía Ramiro, Milena Bond

et al.

Annals of the Rheumatic Diseases, Journal Year: 2023, Volume and Issue: 83(6), P. 741 - 751

Published: Aug. 7, 2023

Objectives To update the EULAR recommendations for use of imaging modalities in primary large vessel vasculitis (LVV). Methods A systematic literature review was performed to retrieve new evidence on ultrasound, MRI, CT and [ 18 F]-fluorodeoxyglucose positron emission tomography (FDG-PET) diagnosis, monitoring outcome prediction LVV. The task force consisted 24 physicians, health professionals patients from 14 countries. were updated based expert opinion, iterating until voting indicated consensus. level agreement determined by anonymous votes. Results Three overarching principles eight agreed. Compared 2018 version, ultrasound is now recommended as first-line test all with suspected giant cell arteritis, axillary arteries should be included standard examination. As an alternative cranial extracranial can examined FDG-PET or MRI. For Takayasu MRI preferred modality; FDG-PET, are alternatives. Although not routinely follow-up, may used assessing abnormalities LVV relapse, particularly when laboratory markers inflammation unreliable. MR-angiography, CT-angiography long-term structural damage, at sites preceding vascular inflammation. Conclusions 2023 provide up-to-date guidance role diagnosis assessment

Language: Английский

Citations

180
Syphilitic bi-valvular endocarditis and myocarditis: modern tools applied to long-forgotten complications of a re-emerging disease DOI

Louiselle LeBlanc,

D Touati,

Simon Lévesque

et al.

The Lancet Infectious Diseases, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

2
The effectiveness of tocilizumab and its comparison with tumor necrosis factor alpha inhibitors for Takayasu Arteritis: A systematic review and meta-analysis DOI
Durga Prasanna Misra, Kritika Singh, Upendra Rathore

et al.

Autoimmunity Reviews, Journal Year: 2023, Volume and Issue: 22(3), P. 103275 - 103275

Published: Jan. 15, 2023

Language: Английский

Citations

25
Novel and potential future therapeutic options in systemic autoimmune diseases DOI Creative Commons
Lili Balogh,

Katalin Oláh,

Soma Sánta

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: March 15, 2024

Autoimmune inflammation is caused by the loss of tolerance to specific self-antigens and can result in organ-specific or systemic disorders. Systemic autoimmune diseases affect a significant portion population with an increasing rate incidence, which means that essential have effective therapies control these chronic Unfortunately, several patients do not respond at all just partially available conventional synthetic disease-modifying antirheumatic drugs targeted therapies. However, during past few years, some new medications been approved be used real-life clinical settings. Meanwhile, candidates appeared offer promising novel treatment options future. Here, we summarize newly most encouraging drug lupus erythematosus, rheumatoid arthritis, Sjögren’s disease, sclerosis, vasculitis, myositis.

Language: Английский

Citations

10
Exosome miR-199a-5p modulated vascular remodeling and inflammatory infiltration of Takayasu’s arteritis DOI Creative Commons

Shuning Guo,

Jiehan Li,

Shurui Pang

et al.

Arthritis Research & Therapy, Journal Year: 2025, Volume and Issue: 27(1)

Published: Jan. 20, 2025

Advances in treatment have swiftly alleviated systemic inflammation of Takayasu's arteritis (TAK), while subclinical vascular and the ensuing arterial remodeling continue to present unresolved challenges TAK. The phenotypic switching smooth muscle cells (VSMC) is regarded as first step pathology contributes remodeling. Exosomes facilitate transfer exchange proteins specific nucleic acids, thereby playing a significant role intercellular communication. Little known about modulatory serum exosomes VSMC Serum isolated from TAK patients were co-cultured with identify exosomes. transfected miR-199a-5p mimic inhibitor. CCK8 assays EdU performed measure proliferative ability. migration was evaluated by scratch transwell assays. flow cytometry employed apoptosis VSMC. Dual-luciferase reporter assay, RNA immunoprecipitation assay fluorescence situ hybridization utilized validate target gene miR-199a-5p. correlational analysis conducted among exosome miRNA, MMP2, TIMP2 clinical parameters patients. coculture mediated dedifferentiation Through gain- loss-of-function approaches, over-expression significantly increased expression marker genes inhibited proliferation migration, whilst opposite effect observed when endogenous knocked down. overexpression suppressed apoptosis. Further, MMP2 serves functional correlation analyses revealed an inverse between Vasculitis Damage Index level or which requires validation larger cohort. Our study indicated that miR-199a-5p/MMP2 pathway played inhibiting decreased secretion may potentially prompt intimal infiltration inflammatory within wall, offering novel therapeutic opportunity tackling both responses neointimal overgrowth associated damage. Moreover, derived possess potential future biomarkers for injury.

Language: Английский

Citations

1
The IL-6 axis in vascular inflammation: effects of IL-6 receptor blockade on vascular lesions from patients with giant-cell arteritis DOI
Marc Corbera‐Bellalta, Farah Kamberović, Roser Alba-Rovira

et al.

Annals of the Rheumatic Diseases, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Blocking interleukin (IL)-6-receptor with tocilizumab has been a major advance in the treatment of giant-cell arteritis (GCA), supporting crucial role IL-6 receptor signalling. However, nearly half patients are not able to maintain glucocorticoid- free remission tocilizumab. The impact on vascular lesions GCA is largely unknown since conflicting results have obtained by imaging. expression and functional IL-6-receptor immunopathology previously investigated. This study aimed investigate control arteries assess ex vivo-cultured temporal aortic tissue from GCA. used hypothesis-driven, candidate molecule transcriptomic approach using vivo artery culture, quantitative real-time polymerase chain reaction, immunofluorescence, Western Blot, immunoassay, adhesion, chemotaxis assays. protein expressed intensively compared that arteries. Tocilizumab decreased expression/phosphorylation STAT3 reduced STAT3-dependent molecules including suppressor cytokine signalling 3, CCL-2, ICAM-1 cultured GCA-involved patients' peripheral blood mononuclear cells (PBMCs). A similar trend was observed tissue. Consistently, PBMC adhesiveness smooth muscle human umbilical vein endothelial towards supernatants tocilizumab-treated In some specimens, increased STAT1 phosphorylation STAT1-dependent chemokines CXCL9 CXCL10. significant reducing, but abrogating, key involved recruitment. About may activate alternative inflammatory pathways their as potential escape mechanism deserves further investigation.

Language: Английский

Citations

1
Rheumatic Immune-Related Adverse Events due to Immune Checkpoint Inhibitors—A 2023 Update DOI Open Access
Quang Minh Dang, Ryu Watanabe,

Mayu Shiomi

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(6), P. 5643 - 5643

Published: March 15, 2023

With the aging of population, malignancies are becoming common complications in patients with rheumatoid arthritis (RA), particularly elderly patients. Such often interfere RA treatment. Among several therapeutic agents, immune checkpoint inhibitors (ICIs) which antagonize immunological brakes on T lymphocytes have emerged as a promising treatment option for variety malignancies. In parallel, evidence has accumulated that ICIs associated numerous immune-related adverse events (irAEs), such hypophysitis, myocarditis, pneumonitis, and colitis. Moreover, not only exacerbate pre-existing autoimmune diseases, but also cause de novo rheumatic disease–like symptoms, arthritis, myositis, vasculitis, currently termed irAEs. Rheumatic irAEs differ from classical diseases multiple aspects, should be individualized based severity. Close collaboration oncologists is critical preventing irreversible organ damage. This review summarizes current regarding mechanisms management focus vasculitis. Based these findings, potential strategies against discussed.

Language: Английский

Citations

21
Mini review of photoacoustic clinical imaging: a noninvasive tool for disease diagnosis and treatment evaluation DOI Creative Commons
Huazhen Liu, Ming Wang, Fei Ji

et al.

Journal of Biomedical Optics, Journal Year: 2024, Volume and Issue: 29(S1)

Published: Jan. 16, 2024

SignificancePhotoacoustic (PA) imaging is an modality that integrates anatomical, functional, metabolic, and histologic insights. It has been a hot topic of medical research draws extensive attention.AimThis review aims to explore the applications PA clinical in human diseases, highlighting recent advancements.ApproachA systemic survey literature concerning utility was conducted, with particular focus on its application tumors, autoimmune inflammatory conditions, endocrine disorders.ResultsPA emerging as valuable tool for disease investigation. Information provided by can be used diagnosis, grading, prognosis multiple types tumors including breast ovarian neoplasms, thyroid nodules, cutaneous malignancies. facilitates monitoring activity diseases such rheumatoid arthritis, sclerosis, arteritis, bowel capturing dynamic functional alterations. Furthermore, unique capability visualizing vascular structure oxygenation levels aids assessing diabetes mellitus comorbidities function.ConclusionsDespite extant challenges, offers promising noninvasive precision long-term evaluation, anticipation, making it potentially significant practice.

Language: Английский

Citations

7
Single-cell analysis in rheumatic and allergic diseases: insights for clinical practice DOI
Masayuki Nishide, Hiroshi Shimagami, Atsushi Kumanogoh

et al.

Nature reviews. Immunology, Journal Year: 2024, Volume and Issue: 24(11), P. 781 - 797

Published: June 24, 2024

Language: Английский

Citations

7
Perspectives of JAK Inhibitors for Large Vessel Vasculitis DOI Creative Commons
Ryu Watanabe, Motomu Hashimoto

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: March 30, 2022

Vasculitis is an inflammation of the blood vessels caused by autoimmunity and/or autoinflammation, and recent advances in research have led to a better understanding its pathogenesis. Glucocorticoids cyclophosphamide long been standard care. However, B-cell depletion therapy with rituximab has become available for treating antineutrophil cytoplasmic antibody-associated vasculitis (AAV). More recently, avacopan, inhibitor complement 5a receptor, was shown high efficacy remission induction against AAV. Thus, treatment options AAV expanded. In contrast, large vessel (LVV), including giant cell arteritis Takayasu arteritis, tocilizumab, IL-6 receptor antagonist, be effective suppressing relapse steroid-sparing effects. rate remains high, other therapeutic awaited. last decade, Janus kinase (JAK) inhibitors emerged as rheumatoid arthritis (RA). Their proven multiple studies; thus, JAK are expected promising agents rheumatic diseases, LVV. This mini-review briefly introduces mechanism action their patients RA. Then, pathophysiology LVV updated, rationale provided brief introduction our preliminary results using mouse model. Finally, we discuss newly raised safety concerns regarding future perspectives

Language: Английский

Citations

26