Ecology Letters,
Journal Year:
2023,
Volume and Issue:
26(7), P. 1174 - 1185
Published: May 10, 2023
Suppression
gene
drives
bias
their
inheritance
to
spread
through
a
population,
potentially
eliminating
it
when
they
reach
high
frequency.
CRISPR
homing
suppression
have
already
seen
success
in
the
laboratory,
but
several
models
predict
that
may
be
elusive
population
with
realistic
spatial
structure
due
extinction-recolonization
cycles.
Here,
we
extend
our
continuous
space
framework
include
two
competing
species
or
predator-prey
pairs.
We
find
both
general
and
mosquito-specific
models,
predators
can
facilitate
drive-based
suppression,
albeit
at
cost
of
an
increased
rate
drive
loss
outcomes.
These
results
are
robust
mosquito
seasonal
fluctuations.
Our
study
illustrates
difficulty
predicting
outcomes
complex
ecosystems.
However,
promising
for
prospects
less
powerful
successfully
eliminate
target
other
pest
populations.
Trends in Genetics,
Journal Year:
2023,
Volume and Issue:
39(7), P. 545 - 559
Published: Feb. 16, 2023
The
availability
of
public
genomic
resources
can
greatly
assist
biodiversity
assessment,
conservation,
and
restoration
efforts
by
providing
evidence
for
scientifically
informed
management
decisions.
Here
we
survey
the
main
approaches
applications
in
conservation
genomics,
considering
practical
factors,
such
as
cost,
time,
prerequisite
skills,
current
shortcomings
applications.
Most
perform
best
combination
with
reference
genomes
from
target
species
or
closely
related
species.
We
review
case
studies
to
illustrate
how
facilitate
research
across
tree
life.
conclude
that
time
is
ripe
view
fundamental
integrate
their
use
a
practice
genomics.
Cell,
Journal Year:
2024,
Volume and Issue:
187(5), P. 1076 - 1100
Published: Feb. 1, 2024
Genome
editing
has
been
a
transformative
force
in
the
life
sciences
and
human
medicine,
offering
unprecedented
opportunities
to
dissect
complex
biological
processes
treat
underlying
causes
of
many
genetic
diseases.
CRISPR-based
technologies,
with
their
remarkable
efficiency
easy
programmability,
stand
at
forefront
this
revolution.
In
Review,
we
discuss
current
state
CRISPR
gene
technologies
both
research
therapy,
highlighting
limitations
that
constrain
them
technological
innovations
have
developed
recent
years
address
them.
Additionally,
examine
summarize
landscape
applications
context
health
therapeutics.
Finally,
outline
potential
future
developments
could
shape
coming
years.
Proceedings of the National Academy of Sciences,
Journal Year:
2023,
Volume and Issue:
120(25)
Published: June 12, 2023
Genetic-based
methods
offer
environmentally
friendly
species-specific
approaches
for
control
of
insect
pests.
One
method,
CRISPR
homing
gene
drive
that
target
genes
essential
development,
could
provide
very
efficient
and
cost-effective
control.
While
significant
progress
has
been
made
in
developing
drives
mosquito
disease
vectors,
little
with
agricultural
Here,
we
report
the
development
evaluation
split
doublesex
(dsx)
Drosophila
suzukii,
an
invasive
pest
soft-skinned
fruits.
The
component,
consisting
dsx
single
guide
RNA
DsRed
genes,
was
introduced
into
female-specific
exon
dsx,
which
is
function
females
but
not
males.
However,
most
strains,
hemizygous
were
sterile
produced
male
transcript.
With
a
modified
included
optimal
splice
acceptor
site,
from
each
four
independent
lines
fertile.
High
transmission
rates
(94
to
99%)
observed
line
expressed
Cas9
two
nuclear
localization
sequences
D.
suzukii
nanos
promoter.
Mutant
alleles
small
in-frame
deletions
near
cut
site
functional
thus
would
resistance
drive.
Finally,
mathematical
modeling
showed
strains
be
used
suppression
lab
cage
populations
repeated
releases
at
relatively
low
release
ratios
(1:4).
Our
results
indicate
potentially
effective
means
populations.
Nature Communications,
Journal Year:
2022,
Volume and Issue:
13(1)
Published: Jan. 12, 2022
Abstract
A
recurring
target-site
mutation
identified
in
various
pests
and
disease
vectors
alters
the
voltage
gated
sodium
channel
(
vgsc
)
gene
(often
referred
to
as
knockdown
resistance
or
kdr
confer
commonly
used
insecticides,
pyrethroids
DDT.
The
ubiquity
of
mutations
poses
a
major
global
threat
continued
use
insecticides
means
for
vector
control.
In
this
study,
we
generate
common
isogenic
laboratory
Drosophila
strains
using
CRISPR/Cas9
editing.
We
identify
differential
sensitivities
permethrin
DDT
versus
deltamethrin
among
these
mutants
well
contrasting
physiological
consequences
two
different
mutations.
Importantly,
apply
CRISPR-based
allelic-drive
replace
resistant
with
susceptible
wild-type
counterpart
population
cages.
This
successful
proof-of-principle
opens-up
numerous
possibilities
including
targeted
reversion
insecticide-resistant
populations
native
state
replacement
malaria
transmitting
mosquitoes
those
bearing
naturally
occurring
parasite
alleles.
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: May 29, 2024
Abstract
Gene
drive
systems
could
be
a
viable
strategy
to
prevent
pathogen
transmission
or
suppress
vector
populations
by
propagating
alleles
with
super-Mendelian
inheritance.
CRISPR-based
homing
gene
drives
convert
wild
type
into
in
heterozygotes
Cas9
and
gRNA.
It
is
thus
desirable
identify
promoters
that
yield
high
conversion
rates,
minimize
the
formation
rate
of
resistance
both
germline
early
embryo,
limit
somatic
expression.
In
Drosophila
,
nanos
promoter
avoids
leaky
expression,
but
at
cost
embryo
from
maternally
deposited
Cas9.
To
improve
efficiency,
we
test
eleven
melanogaster
promoters.
Some
achieve
higher
efficiency
minimal
resistance,
none
completely
avoid
However,
such
expression
often
does
not
carry
detectable
fitness
costs
for
rescue
targeting
haplolethal
gene,
suggesting
conversion.
Supporting
4-gRNA
suppression
drive,
one
leads
low
equilibrium
frequency
due
other
outperforms
resulting
successful
cage
population.
Overall,
these
hold
advantages
species
may
possess
valuable
homologs
organisms.
Cell,
Journal Year:
2024,
Volume and Issue:
187(4), P. 931 - 944.e12
Published: Feb. 1, 2024
Differentiation
is
crucial
for
multicellularity.
However,
it
inherently
susceptible
to
mutant
cells
that
fail
differentiate.
These
mutants
outcompete
normal
by
excessive
self-renewal.
It
remains
unclear
what
mechanisms
can
resist
such
expansion.
Here,
we
demonstrate
a
solution
engineering
synthetic
differentiation
circuit
in
Escherichia
coli
selects
against
these
via
biphasic
fitness
strategy.
The
provides
tunable
production
of
analogs
stem,
progenitor,
and
differentiated
cells.
resists
mutations
coupling
the
an
essential
enzyme,
thereby
disadvantaging
non-differentiating
mutants.
selected
maintained
positive
rate
long-term
evolution.
Surprisingly,
this
remained
constant
across
vast
changes
growth
conditions.
We
found
transit-amplifying
(fast-growing
progenitors)
underlie
environmental
robustness.
Our
results
provide
insight
into
stability
powerful
method
evolutionarily
stable
multicellular
consortia.
