Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
Nature reviews. Immunology, Journal Year: 2024, Volume and Issue: unknown
Published: June 3, 2024
Language: Английский
Citations
41
Molecular Biomedicine, Journal Year: 2025, Volume and Issue: 6(1)
Published: March 4, 2025
Abstract Despite the declared end of COVID-19 pandemic, SARS-CoV-2 continues to evolve, with emerging JN.1-derived subvariants (e.g., KP.2, KP.3) compromising efficacy current XBB.1.5-based vaccines. To address this, we developed an mRNA vaccine encoding full-length spike protein JN.1, incorporating GSAS and 2P mutations encapsulated in lipid nanoparticles (LNPs). The JN.1-mRNA elicited robust humoral cellular immune responses mice, including high JN.1-specific IgG titers, cross-neutralizing antibodies, increased T follicular helper (Tfh) cells, germinal center (GC) B cell cytokines. Importantly, immunity persisted for up six months induced RBD-specific long-lived plasma cells. We also compared by homologous heterologous vaccination regimens, our results demonstrated that regimen—combining a recombinant (RBD JN.1 -HR)—induced stronger responses. These findings highlight constitutes effective prophylactic approach against JN.1-related variants, as it induces potent neutralizing antibody across all tested lineages. This enhanced immunogenicity is expected significantly reduce hospitalization rates mitigate post-COVID complications associated KP.3 infections. study emphasizes need timely updates adaptability vaccines addressing pathogens, providing framework combating future infectious diseases. Collectively, these offer critical insights design public health strategies response variants.
Language: Английский
Citations
1
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: May 10, 2024
Abstract Bone marrow plasma cells (BMPCs) produce durable, protective IgM, IgG, and IgA antibodies, in some cases, pro-allergic IgE but their properties sources are unclear. We charted single BMPC transcriptional clonal heterogeneity food-allergic non-allergic individuals across CD19 protein expression given its inverse correlation to longevity. Transcriptional diversity revealed distinct functional profiles. Additionally, distribution of somatic hypermutation intraclonal antibody sequence variance suggest that CD19low CD19high BMPCs arise from recalled memory germinal center B cells, respectively. Most were peanut-allergic individuals; two out 32 independent donors bound peanut antigens vitro vivo. These findings shed light on origins highlight the bone as a source pathogenic allergy.
Language: Английский
Citations
4
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 29, 2025
Abstract Although IgA + long-lived plasma cells (LLPCs) generated following mucosal viral infection provide durable protection against reinfection, little is known about their generation. Here, we show that oral RV induces gut-resident LLPCs produce highly mutated protective IgA. Unlike RV-specific IgG LLPCs, were independently of MHCII expression by dendritic – rather B was both necessary and sufficient. cell also sufficient to induce a unique population T-bet follicular helper T (T FH 1) which crucial for LLPC accumulation in the gut via IFNγ- CXCR3-dependent mechanisms. Similar infection, 1 required influenza-specific response. However, unlike not suggesting operation site-specific priming Collectively, our data reveal unconventionally primed support responses infections.
Language: Английский
Citations
0
Journal of Muscle Research and Cell Motility, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 18, 2025
Abstract Under normal physiological conditions, leukocytes and other tissue resident immune cells have been shown to migrate using the mesenchymal (integrin/adhesion dependent) and/or ameboid independent) modes of migration. The objective this manuscript is provide a comprehensive literature review that illustrates how display high levels plasticity shifting between migration during haematopoiesis inflammatory response. This shaped by reciprocal regulation pattern gene expression associated with their haematopoietic lineage or leukocyte activation status, response physicochemical topological characteristics surrounding tissue. use some common elements from F-actin polymerising actomyosin machinery in both may facilitate capacity alternate two while navigating highly heterogenous landscape cues anatomical journey. We discuss paradigm detailed examples specific populations such as dendritic cells, macrophages lymphocytes. propose cell adhesions involved represent signalling hubs where differentiation converge. These molecular complexes then generate outputs coordinate expansion, differentiation, optimal patterns recruitment inflammation sites.
Language: Английский
Citations
0
Cell Reports Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 102035 - 102035
Published: March 1, 2025
Language: Английский
Citations
0
Immunological Reviews, Journal Year: 2025, Volume and Issue: 331(1)
Published: April 11, 2025
ABSTRACT Upon activation, B cells undergo either the germinal center (GC) or extrafollicular (EF) response. While GC are known to generate high‐affinity memory and long‐lived plasma cells, role of EF response is less well understood. Initially, it was thought be limited that a source fast but lower‐quality antibodies until can form. However, recent evidence strongly supports as an important component humoral infection. responses now also recognized pathogenic in autoimmune diseases. The itself dynamic regulated by pathways only recently being uncovered. We have identified cytokine IL‐12 acts molecular switch, enhancing suppressing through multiple mechanisms. These include direct effects on both themselves coordinated differentiation helper CD4 T cells. Here, we explore this pathway relation other advancements our understanding response's highlight areas for future research. A better how forms essential advancing treatments many disease states.
Language: Английский
Citations
0
Science Advances, Journal Year: 2025, Volume and Issue: 11(16)
Published: April 16, 2025
Antibodies play a pivotal role in the immune defense and long-term immunity. Yet, while several studies have highlighted persistence of antigen-specific antibody responses, it is unclear whether this stems from continuous production same clones or recurrent activation B cells generating new clones. To examine stability human repertoire, we monitored concentrations most abundant IgG1 plasma samples 11 healthy donors at nine sampling points over year. During year, each donor received three doses COVID-19 vaccine. Notwithstanding these vaccinations, remained constant. Given 2- to 3-week half-life molecules blood, our data suggest that are associated with immunity do not undergo somatic hypermutation which would imply short-lived cells. Overall, persistently produced by long-lived
Language: Английский
Citations
0
Cell Research, Journal Year: 2025, Volume and Issue: unknown
Published: May 8, 2025
Language: Английский
Citations
0
mAbs, Journal Year: 2025, Volume and Issue: 17(1)
Published: May 26, 2025
Hybridomas, the first method for creating monoclonal antibodies (mAbs), were reported 50 years ago. This approach, which transformed biomedical research and laid foundation many of current therapeutic, diagnostic, reagent applications mAbs, is still used today, despite low fusion yields between short-lived B cells immortal myeloma cells. To improve hybridoma production accelerate development new we addressed two key limitations: 1) random pairing antibody-producing cells, 2) efficiency polyethylene-glycol-mediated process. We characterized isolated antibody-secreting (ASCs) from spleen immunized mice before cell to increase probability successive most suitable partners favor generation functional hybridomas. Specifically, developed an optimized workflow combining fluorescence-activated sorting with antibody secretion assays, using a panel five cell-surface markers (CD3, TACI, CD138, MHC-II, B220) identify distinct ASC subset characteristics. Such ASCs exhibited plasmablast phenotype high MHC-II expression secreted levels antigen (Ag)-specific in mice. then implemented electrofusion procedure adapted numbers (<106 cells), order perform targeted TACIhighCD138high sorted ASCs. approach yielded viable hybridomas 100% seeded culture wells compared only 40% unsorted In particular, over 60% generated Ag-specific including IgGs Ag binding affinity (<10-9 M). These results pave way high-yield mAb via fusion, potential streamline thereby expand access mAbs.
Language: Английский
Citations
0