Stepwise neofunctionalization of the NF-κB family member Rel during vertebrate evolution DOI Creative Commons
Allison E. Daly, Anne B. Chang, Prabhat Kumar Purbey

et al.

Nature Immunology, Journal Year: 2025, Volume and Issue: 26(5), P. 760 - 774

Published: April 30, 2025

Adaptive immunity and the five vertebrate NF-κB family members first emerged in cartilaginous fish, suggesting that divergence helped to facilitate adaptive immunity. One specialized function of Rel protein macrophages is activation Il12b, which encodes a key regulator T cell development. We found Il12b exhibits much greater dependence than inducible innate genes macrophages, with unique dimers depending on heightened intrinsic DNA-binding affinity. Chromatin immunoprecipitation followed by sequencing experiments defined differential preferences genome-wide, X-ray crystallography revealed residue supports affinity dimers. Unexpectedly, this residue, dimers, portion promoter bound were largely restricted mammals. Our findings reveal major structural transitions an member one its target promoters at late stage evolution apparently contributed immunoregulatory rewiring mammalian species.

Language: Английский

Vertebrate TNF Superfamily: Evolution and Functional Insights DOI Creative Commons
Ignacio Marı́n

Biology, Journal Year: 2025, Volume and Issue: 14(1), P. 54 - 54

Published: Jan. 10, 2025

This study characterizes the evolution of tumor necrosis factor superfamily (TNFSF) across vertebrate lineages, both cyclostomes and gnathostomes, by combining sequence similarity synteny data for genes from 23 model species. The available evidence supports a simple in which most diversity found living species can be attributed to expansion four an ancestor all vertebrates before first genome duplications that occurred lineages. It is inferred possessed only six TNFSF genes. A cyclostome-specific triplication had little effect on total number these due second duplication plus additional single-gene duplications, already 21 In several gnathostome particularly some tetrapods, TNF has significantly contracted numerous gene losses. evolutionary provides framework exploring functional data, showing descendants different ancestral have acquired distinct roles, prominently innate adaptive immune systems, led species-specific refinement were conserved or lost. Several hitherto difficult interpret (the interactions very ligands with same receptors; ability bind alternative receptors, without death domains; cooperation specific functions) explained as consequences history superfamily.

Language: Английский

Citations

0
Stepwise neofunctionalization of the NF-κB family member Rel during vertebrate evolution DOI Creative Commons
Allison E. Daly, Anne B. Chang, Prabhat Kumar Purbey

et al.

Nature Immunology, Journal Year: 2025, Volume and Issue: 26(5), P. 760 - 774

Published: April 30, 2025

Adaptive immunity and the five vertebrate NF-κB family members first emerged in cartilaginous fish, suggesting that divergence helped to facilitate adaptive immunity. One specialized function of Rel protein macrophages is activation Il12b, which encodes a key regulator T cell development. We found Il12b exhibits much greater dependence than inducible innate genes macrophages, with unique dimers depending on heightened intrinsic DNA-binding affinity. Chromatin immunoprecipitation followed by sequencing experiments defined differential preferences genome-wide, X-ray crystallography revealed residue supports affinity dimers. Unexpectedly, this residue, dimers, portion promoter bound were largely restricted mammals. Our findings reveal major structural transitions an member one its target promoters at late stage evolution apparently contributed immunoregulatory rewiring mammalian species.

Language: Английский

Citations

0