Synthetic
long
oligodeoxynucleotides
(ODNs)
have
found
wide
applications
in
diverse
fields
such
as
chemical
biology,
synthetic
and
genes
genomes
synthesis.
Those
leading
to
a
significant
demand
for
their
production.
However,
traditional
methods
purifying
ODNs
present
notable
drawbacks,
particularly
inability
purify
ODNs,
rendering
ODN
synthesis
challenging.
To
address
these
issues,
techniques
coupled
with
non-chromatographic
purification
been
developed.
This
technology
makes
the
of
feasible
offers
viable
pathway
producing
extensive
repeats
or
stable
secondary
structures,
which
are
either
unattainable
exceedingly
complex
achieve
using
conventional
methods.
Despite
advancements,
use
controlled
pore
glass
(CPG)
solid
support
poses
its
own
limitations.
In
response,
novel
surface-functionalized
materials
identified
mitigate
challenges.
Direct
up
1,728-mer
on
surface
has
reported.
Results
indicate
that
surfaces
eliminates
steric
hindrance
reduces
errors
commonly
encountered
CPG.
Furthermore,
while
catching-by-polymerization
(CBP)
method
proven
effective,
affordability
polymerizable
tagging
phosphoramidites
(PTPs)
remains
bottleneck.
overcome
this
hurdle,
scalable
four
nucleoside
precursors
discovered,
offering
chromatography-free
alternatives
making
CBP
more
efficient
cost-effective.
Journal of Industrial Microbiology & Biotechnology,
Journal Year:
2024,
Volume and Issue:
51
Published: Jan. 1, 2024
Abstract
Effective
microbial
bioprocessing
relies
on
maintaining
ideal
cultivation
conditions,
highlighting
the
necessity
for
tools
that
monitor
and
regulate
cellular
performance
robustness.
This
study
evaluates
a
fed-batch
control
system
based
at-line
flow
cytometry
monitoring
of
intact
yeast
cells
having
fluorescent
transcription
factor-based
redox
biosensor.
Specifically,
biosensor
assesses
response
an
industrial
xylose-fermenting
Saccharomyces
cerevisiae
strain
carrying
TRX2p-yEGFP
NADPH/NADP+
ratio
imbalance
when
exposed
to
furfural.
The
developed
successfully
detected
output
automatically
adjusted
furfural
feed
rate,
ensuring
physiological
fitness
at
high
levels.
Moreover,
single-cell
measurements
enabled
subpopulation
dynamics,
enhancing
precision
over
traditional
methods.
presented
automated
highlights
potential
combining
biosensors
robust
cultivations
by
leveraging
intracellular
properties
as
inputs.
One-Sentence
Summary
An
using
enhances
regulating
in
environmental
stressor
Chapter
1
function
(Cleland
2011).Stability
of
these
properties
also
contributes
to
function,
which
I
detail
later
in
this
introduction
under
"Community
stability".General
ecological
theories
and
associated
definitions,
were
mostly
formulated
with
multicellular
eukaryotes
mind,
apply
microbes
(Mallon,
Elsas
Salles
2015;
Prosser
Martiny
2020).
Microbial
communities
&
metabolic
functions:Although
unseen
our
naked
eyes,
microbial
offer
us
intricate
yet
manipulatable
ecosystems
investigate
core
questions
about
community
composition
function.One
may
easily
imagine
rainforests,
savannahs,
ocean
reefs
when
thinking
diverse
communities,
but
similarly
complex
interactions
including
competition
for
resources,
cooperation,
predation,
shape
the
communities.The
great
value
both
natural
human-desired
functions
is
rapidly
being
recognised.The
power
has
long
been
utilised
by
humankind,
while
traditional
uses
naturally
occurring
continues
(i.e.,
food
fermentation,
composting),
engineering
approaches
are
advancing
develop
selected
compositions
specified
(Sivasubramaniam
Franks
2016;
Jones,
Marken
Silver
2024).
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 17, 2024
Abstract
The
engineering
of
genetic
circuits
to
perform
predefined
computations
is
central
synthetic
biology,
enabling
living
cells
with
new
functionalities
applicable
across
various
domains.
However,
these
are
often
specifically
tailored
particular
cellular
hosts,
Escherichia
coli
being
the
most
popular.
Consequently,
their
intended
functions
may
not
translate
well
other
organisms,
limiting
scope.
Understanding
circuit
dynamics
in
less
familiar
organisms
crucial,
especially
for
niche-specific
applications
requiring
chassis
different
from
model
generally
used
biology.
Here,
we
develop
a
combined
experimental
and
theoretical
pipeline
evaluate
performance
NOT
logic
circuits,
also
called
inverters,
soil
bacterium
Pseudomonas
protegens
Pf-5—a
host
renowned
its
unique
environmental
newcomer
circuitry.
Inverters
were
experimentally
tested
characterize
input-output
functionality,
mathematical
modelling
was
infer
dynamic
principles
modules.
quantified
individual
impacts
key
parameters—such
as
translation
efficiency,
repressor
performance,
promoter
activity—on
output
levels,
predictions
about
inter-host
portability.
This
parameter
calibration
revealed
properties
chassis,
including
faster
transitions
between
on
off
states
compared
biology
workhorse
putida
.
These
characteristics
reflect
adaptations
fluctuating
conditions
plant
rhizosphere,
where
this
bacteria
thrives.
As
result,
our
work
provides
DNA
parts,
characterizations
establish
P.
Pf-5
viable
Journal of Sustainable Agriculture and Environment,
Journal Year:
2024,
Volume and Issue:
3(4)
Published: Oct. 1, 2024
ABSTRACT
The
increasing
severity
of
global
climate
change
has
led
to
more
frequent
extreme
high‐temperature
events,
significantly
damaging
rice
yield
and
quality,
thus
posing
a
threat
food
security.
Research
indicates
that
plant‐microbe
interactions
can
enhance
plant
growth
overall
health
under
adverse
conditions.
Therefore,
this
review
aims
explore
strategies
improve
heat
tolerance
through
thermophilic
microorganism
mediation.
This
paper
systematically
summarises
the
effects
stress
on
both
aboveground
underground
parts
during
its
stages,
identifies
molecular
mechanisms
by
which
responds
stress,
explores
potential
roles
microorganisms.
Additionally,
we
existing
studies
microorganisms
alleviate
their
action.
Through
case
studies,
how
survival
in
environments
regulating
development,
along
with
applications
sustainable
agriculture.
In
future,
environmentally
friendly
efficient
microbial
inoculants
biofertilizers
are
expected
be
developed
based
microbe‐mediated
mechanisms,
will
help
mitigate
challenges
crops
face
change.
Synthetic
long
oligodeoxynucleotides
(ODNs)
have
found
wide
applications
in
diverse
fields
such
as
chemical
biology,
synthetic
and
genes
genomes
synthesis.
Those
leading
to
a
significant
demand
for
their
production.
However,
traditional
methods
purifying
ODNs
present
notable
drawbacks,
particularly
inability
purify
ODNs,
rendering
ODN
synthesis
challenging.
To
address
these
issues,
techniques
coupled
with
non-chromatographic
purification
been
developed.
This
technology
makes
the
of
feasible
offers
viable
pathway
producing
extensive
repeats
or
stable
secondary
structures,
which
are
either
unattainable
exceedingly
complex
achieve
using
conventional
methods.
Despite
advancements,
use
controlled
pore
glass
(CPG)
solid
support
poses
its
own
limitations.
In
response,
novel
surface-functionalized
materials
identified
mitigate
challenges.
Direct
up
1,728-mer
on
surface
has
reported.
Results
indicate
that
surfaces
eliminates
steric
hindrance
reduces
errors
commonly
encountered
CPG.
Furthermore,
while
catching-by-polymerization
(CBP)
method
proven
effective,
affordability
polymerizable
tagging
phosphoramidites
(PTPs)
remains
bottleneck.
overcome
this
hurdle,
scalable
four
nucleoside
precursors
discovered,
offering
chromatography-free
alternatives
making
CBP
more
efficient
cost-effective.
