Cited by Hybrid proteins: fusion chimeras and natural wonders

Phage CRISPR-like regulatory RNAs silence bacterial adaptive and innate immunity DOI

Chao Liu,

Rui Wang, Zhihua Li

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

Abstract In prokaryotes, arrayed CRISPR RNAs (crRNAs) guide Cas proteins to destroy phage DNA/RNA, while solitary crRNA-like (crlRNAs) program for auto-regulation or control abortive infection (Abi)-inducing toxins that activate when CRISPR-Cas fails. Here, we report phages exploit crlRNA mimics hijack these multi-layered host defenses. Pseudomonas aeruginosa use crlRNAs thwart immunity by inhibiting expression, block Abi silencing an unprecedented RNA toxin features consecutive proline codons. Remarkably, the anti-CRISPR protein AcrIF24 selectively inhibits loaded with crRNAs/crlRNAs, allowing those complexed viral synergistically responses, as have co-adaptively evolved shorter spacer sequences. Furthermore, frequently organize multiplexed arrays, mirroring architecture of bacterial CRISPRs. Our findings showcase how silence defenses, and highlight delicate synergy between protein-based strategies.

Language: Английский

Citations

Amyloid signaling in antiphage defense DOI

Léa Ibarlosa,

Sonia Dheur,

C. Sánchez

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

Immune regulated cell death (RCD) is a defense strategy common to different domains of life involving purposeful sacrifice infected cells. In animals and fungi, functional amyloids play role in the control RCD as molecular switches activating key effectors, rather than causing direct toxicity seen with pathological amyloids. Here, we describe novel amyloid-based signal transduction mechanism an antiphage system Escherichia coli. This abortive infection (Abi) mediated by two proteins, Bab Agp which share amyloid motif are encoded adjacent genes. Upon phage infection, activates through signaling, leading death. We determined structure execution domain, distantly related pore-forming present plants. show that fungal HET-S amyloid-controlled protein functionally interchangeable their respective roles allorecognition. These findings add repertoire

Language: Английский

Citations

Continuing Discoveries in Immunogenetics and Computational Immunology: An Update DOI

Giulia Russo, Elena Crispino, Esther M. Lafuente

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

Hybrid proteins: fusion chimeras and natural wonders DOI

Muhamed Adilović, Jasmin Šutković, Altijana Hromić‐Jahjefendić

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 79 - 98

Published: Nov. 22, 2024

Language: Английский

Citations